Isolation and Characterization of Chitinolytic Bacteria for Chitinase Production from the African Catfish, Clarias gariepinus (Burchell, 1822)

Ajayi, A. A.; Adeola, Onibokun Elizabeth; George, F.O.A.; Atolagbe, O. M.

doi:10.3923/jm.2016.119.125

Cited by 15 publications

(5 citation statements)

References 14 publications

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“…Aeromonas sp. CQNU6-2's crude chitinase activity was active over a broad pH range from 3 to 10, with the maximum activity observed at pH 6, showing potential in the biological control of pests and diseases (Ajayi et al, 2016). Moreover, the enzyme exhibited chitin hydrolysis capability across a wide temperature range of 10 to 90°C.…”

Section: Chitinase Applicationmentioning

confidence: 99%

Red Sea Chitinase-Producing Enterococcus sp.: Isolation, Characterization, and Application

kelany et al.

2024

Egypt. J. of Aquatic Biolo. and Fish.

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Section: Chitinase Applicationmentioning

confidence: 99%

Red Sea Chitinase-Producing Enterococcus sp.: Isolation, Characterization, and Application

kelany et al.

2024

Egypt. J. of Aquatic Biolo. and Fish.

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“…is due to the antimicrobial substance (Phenazine-1-carboxylic acid) produced by P. fluorescens LBUM223, which interferes with the proper cellular function of the pathogen and so results in its inhibition [ 14 ]. Antimicrobial substances like 2,4-diacetylphloroglucinol, hydrogen cyanide, chitinase, phenazines, organic acids, as well as iron-chelating substances (siderophores) are responsible for the induction and maintenance of a disease suppressive soil [ 15 , 16 , 17 , 18 ]. In this study, we evaluated the functional genes profile abundance involved in chemotaxis and antimicrobial and siderophore producing substances from the maize rhizosphere under organic, inorganic, and untreated control using shotgun metagenomics study.…”

Section: Introductionmentioning

confidence: 99%

Metagenomics Assessment of Soil Fertilization on the Chemotaxis and Disease Suppressive Genes Abundance in the Maize Rhizosphere

Enebe

Babalola

2021

Genes

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Soil fertility is a function of the level of organic and inorganic substances present in the soil, and it influences the activities of soil-borne microbes, plant growth performance and a host of other beneficial ecological functions. In this metagenomics study, we evaluated the response of maize microbial functional gene diversity involved in chemotaxis, antibiotics, siderophores, and antifungals producing genes within the rhizosphere of maize plants under compost, inorganic fertilizer, and unfertilized conditions. The results show that fertilization treatments at higher compost manure and lower inorganic fertilizer doses as well as maize plants itself in the unfertilized soil through rhizosphere effects share similar influences on the abundance of chemotaxis, siderophores, antifungal, and antibiotics synthesizing genes present in the samples, while higher doses of inorganic fertilizer and lower compost manure treatments significantly repress these genes. The implication is for a disease suppressive soil to be achieved, soil fertilization with high doses of compost manure fertilizer treatments as well as lower inorganic fertilizer should be used to enrich soil fertility and boost the abundance of chemotaxis and disease suppressive genes. Maize crops also should be planted sole or intercropped with other crops to enhance the rhizosphere effect of these plants in promoting the expression and abundance of these beneficial genes in the soil.

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“…Microorganisms with chitinolytic abilities are believed to be able to play a role in controlling the attack of pathogenic fungi by making chitin a source of carbon and nitrogen (Jholapara, Mehta, Bhagwat, and Sawant, 2013). Several groups of bacteria and fungi with chitinolytic abilities are used in controlling plant pathogens such as Bacillus (Wibowo, Mubarik, Rusmana, Thenawidjaya, 2017, Pseudomonas (Saranya et al, 2013), Trichoderma (Agrawal et al, 2012, Toshy et al, 2012 and Streptomyces (Arias et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Isolation and Screening of Soil Chitinolytic Actinobacteria as the Anti-Fungal Producer of Plant Pathogens

Wibowo

Sipriyadi

Mubarik

et al. 2020

EKW

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Chitinolytic actinobacteria are currently more widely used because of their ability as the biological control agents to the pathogenic fungi, especially in horticultural and plantation crops. This research was conducted to obtain isolates of chitinolytic soil actinobacteria from the rhizosphere of the rubber plant (Hevea brasiliensis) area in IPB University. Antifungal activities from these actinobacteria hopely able to inhibit the growth of plant pathogenic fungi in Vitro on chitin agar media. Fusarium oxysporum and Sclerotium rolfsii are used in the inhibition test of chitinolytic actinobacteria. The results successfully obtained 16 isolates of actinobacteria were grew on Humic Acid Vitamin (HV) agar. It showed that six of 16 actinobacteria isolates were able to produce inhibition zones to the growth of hyphae of pathogenic fungi on potato dextrose agar (PDA). KK-15 and KK-07 isolates were able to produce the largest inhibition percentages in F. oxysporum and S. rolfsii. Based on the chitinolytic index (CI) values, KK-15 and KK-07 isolates produced CI values of 1.25 and 1.5, respectively. The morphological characteristics and Gram staining of both KK-15 and KK-07 isolates are closely relative with Streptomyces sp. Abstrak: Bakteri kitinolitik saat ini banyak diteliti karena kemampuannya sebagai agens pengendali hayati jamur patogen khususnya pada tanaman hortikultura dan perkebunan. Penelitian ini dilakukan untuk mendapatkan isolat-isolat bakteri kitinolitik asal tanah dari bagian perakaran tanaman karet (Hevea brasiliensis) di daerah perkebunan karet Institut Pertanian Bogor (IPB). Aktivitas antifungi dari bakteri ini diharapkan mampu menghambat pertumbuhan jamur patogen tanaman secara in Vitro pada media agar-agar kitin. Fusarium oxysporum dan Sclerotium rolfsii digunakan dalam uji hambat aktinobakteri kitinolitik. Hasil penelitian memperoleh 16 isolat aktinobakteri berhasil diisolasi dengan menggunakan media agar-agar Humic Acid Vitamin (HV), dan dari 16 isolat tersebut, enam isolat mampu menghasilkan zona hambat terhadap pertumbuhan hifa dari kedua jamur patogen pada media Potato Dextrose Agar (PDA) secara in Vitro. Isolat KK-15 dan KK-07 mampu menghasilkan diameter hambatan terbesar pada jamur F. oxysporum dan S. rolfsii. Berdasarkan nilai index kitinolitik (IK), isolat KK-15 dan KK-07 menghasilkan nilai IK sebesar 1,25 dan 1,5 secara berurutan. Karakteristik morfologi dan pewarnaan Gram dari kedua isolat yaitu KK-15 dan KK-07 memiliki kedekatan dengan kelompok Streptomyces spp.

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Isolation and Characterization of Chitinolytic Bacteria for Chitinase Production from the African Catfish, Clarias gariepinus (Burchell, 1822)

Cited by 15 publications

References 14 publications

Red Sea Chitinase-Producing Enterococcus sp.: Isolation, Characterization, and Application

Red Sea Chitinase-Producing Enterococcus sp.: Isolation, Characterization, and Application

Metagenomics Assessment of Soil Fertilization on the Chemotaxis and Disease Suppressive Genes Abundance in the Maize Rhizosphere

Isolation and Screening of Soil Chitinolytic Actinobacteria as the Anti-Fungal Producer of Plant Pathogens

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