The inadequacy of pipe borne water in Ibadan Metropolis is almost endemic. This inadequacy is both in quantity and quality of the public water supply. As an alternative to the water supply, small scale industrial concerns came up with 'PURE WATER': 0.5Litre water in nylon sachets, which are electrically heated, sealed at both ends and widely patronized by both low and middle class Nigerians. The objective of the study was to find out the quality of such pure water. Stratified, simple random sampling procedures were used to select 78 samples from 20 brands of sachet water from important hawkers/vendors. The samples were subjected to physical, chemical and bacteriological analysis. For chemical analysis, Hanna's 100 spectrophotometer plus chemical reagents were used. The water samples were assessed for coliform and other intestinal bacteria using MKC cowkery broth (MM) and Brilliant Green Bile Broth (BGBB). Multiple tube method was employed. The result showed that the physical parameters were within W.H.O limits for drinking water quality guidelines except for pH which ranged from 6.6-9.7. Some chemical parameters were also within the W.H.O guideline values. However; aluminum which concentration ranged from 0.00-0.34 mg/l, fluoride concentration ranged from 0.01-1.87 mg/I and cyanide concentration ranged from 0.000-0175 were not. Bacteriological analysis, showed that five (5) or 6.4% of the samples tested fielded bacterial growth. Bacteria produced included: Klebsiella sp., Streptococcus faecalis and Pseudomonas aeruginosa. The enforcement of the regulation guiding water quality before the National Agency for Food and Drug Administration Control (NAFDAC) to comply with the drinking water qualities guideline values as recommended by W.H.O, becomes urgent.

BackgroundThe resistance of human malaria parasites to anti-malarial compounds has become considerable concern, particularly in view of the shortage of novel classes of anti-malarial drugs. One way to prevent resistance is by using new compounds that are not based on existing synthetic antimicrobial agents.ResultsSensitivity of 100 Plasmodium falciparum isolates to chloroquine, quinine, amodiaquine, mefloquine, sulphadoxine/pyrimethamine, artemisinin, Momordica charantia (‘Ejirin’) Diospyros monbuttensis (‘Egun eja’) and Morinda lucida (‘Oruwo’) was determined using the in vitro microtest (Mark III) technique to determine the IC50 of the drugs. All the isolates tested were sensitive to quinine, mefloquine and artesunate. Fifty-one percent of the isolates were resistant to chloroquine, 13% to amodiaquine and 5% to sulphadoxine/pyrimethamine. Highest resistance to chloroquine (68.9%) was recorded among isolates from Yewa zone while highest resistance to amodiaquine (30%) was observed in Ijebu zone. Highest resistance to sulphadoxine/pyrimethamine was recorded in Yewa and Egba zones, respectively. A positive correlation was observed between the responses to artemisinin and mefloquine (P<0.05), artemisinin and quinine (P<0.05) and quinine and mefloquine (P<0.05). A negative correlation was observed between the responses to chloroquine and mefloquine (P>0.05). Highest anti-plasmodial activity was obtained with the ethanolic extract of D. monbuttensis (IC50 = 3.2nM) while the lowest was obtained from M. lucida (IC50 =25nM).ConclusionsNatural products isolated from plants used in traditional medicine, which have potent anti-plasmodial action in vitro, represent potential sources of new anti-malarial drugs.

Background: Plasmodium falciparum the main causative agent of malaria is an important public health vector. With the use of PCR, its genetic diversity has been extensively studied with dearth information from Nigeria.

The production of tomatoes has experienced a huge rise over the years as a result of its economic, nutritional and cancer reduction importance. Despite the rapid advancement of technology in the past century, storage of tomato fruits remains a major problem experienced in the postharvest chain in most developing countries. This study gives a survey of the various causes of tomatoes postharvest losses, the different methods used in storage of the fruit over time and their limitations. It was found that the conventional methods used in tomatoes storage, improved its shelf life but was accompanied by some significant losses in quantity and quality. Hence, generating a need for a postharvest storage system taking into cognizance the optimum conditions required for the fruit storage. The development of a thermally controlled postharvest storage system with the sole purpose of increasing shelf life and minimize the rate of deterioration becomes inevitable.

This study was carried out to determine the antiplasmodial activity of leaves of Moringa oleifera. Cold extraction method was carried out on grinded leaves to prepare the crude ethanolic and n-hexane extracts. Mice models (Mus musculus) were passaged with chloroquine resistant Plasmodium berghei, which are similar in morphology, physiology and life cycle to P. falciparum that infect humans. Stock solutions of 5 mg/mL 5% DMSO were prepared and the extracts were administered at different treatment concentrations, 50 mg/kg, 100 mg/kg and 200 mg/kg body weight over 4 days. Positive and negative control groups, Chloroquine diphosphate (25 mg/kg) and 5% DMSO, respectively were set up. Crude ethanolic and n-hexane extracts of M. oleifera showed anti-plasmodial activity at the three different concentrations used. Both crude ethanolic and nhexane extracts of M. oleifera leaves showed a significant inhibition of parasitaemia (p < 0.05) ranging from 74.7 to 95.6% for ethanolic extract and 59.3 to 87.9% for n-hexane extract. EC50 value of crude ethanolic and n-hexane extracts were 32 mg/kg and 42 mg/kg body weight, respectively. M. oleifera showed potential for possible future use as an alternative to some conventional drugs.