In vivo Antiplasmodial Activity of Crude Ethanolic and N-hexane Extracts of Moringa oleifera Leaves

Olasehinde, G. I; Ayanda, Opeyemi Isaac; Egwari, L. O.; Ajayi, A. A.; Awofeso, T.

doi:10.17957/ijab/15.0161

Cited by 16 publications

(13 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The extract gave a significant (P<0.05) non dose dependent reduction in the mean percentage parasitemia levels. This current finding contradicts the research result reported by Olashinde [32] involving Moringa oleifera seed which showed that the in vivo anti-malarial reduction of the mean % parasitemia was dose dependent for the n-hexane and ethanol extracts ( Table 3).…”

Section: Resultscontrasting

confidence: 99%

Anti-plasmodial Effect of Moringa oleifera Seeds in Plasmodium berghei Infected Albino Rats

Ga¹,

Christian²

2020

Biochem Pharmacol

View full text Add to dashboard Cite

This study was carried out to investigate the anti-plasmodial effect of Moringa oleifera seeds in Plasmodium berghei infected albino rats to affirm its traditional use in the treatment of malaria in Nigeria and some African countries. The pods of this plant were collected from a garden in Port Harcourt, Nigeria and authenticated by a taxonomist. The seed were dried under shade, deshelled, crushed into powder and extracted with 99% ethanol using rotary evaporator and water bath. Phytochemical investigation was conducted on the powdered seeds using standard method to determine the presence of the bioactive compounds and oral acute toxicity test was also carried out using the modified method of Karber. The in-vivo anti-plasmodial effect of the plant extract of Moringa oleifera (200, 300 and 500 mg/kg), Artemether/Lumefantrine (A/L), Artesunate/Mefloquine (A/M) and Chloroquine (CQ) against chloroquine sensitive strain of Plasmodium berghei in infected albino rats (120-130 g) were investigated using a 4 day range test for curative effect and prophylactic model. Standard methods were used to determine the percentage parasitemia. A non-dose dependent significant (P<0.05) parasitemia suppression was discovered after an oral treatment with the ethanol extract of the seed plant at different dose levels as compared to the control group. However, 300 mg-kg-1 (72.56 ± 1.75%) of the extracts exhibited a relatively higher chemosupression compared to 200 mg-kg-1 (78.55 ± 2.42%), 300 mg-kg-1 (79.15 ± 0.72%) and 500 mg-kg-1 (77.60 ± 1.53%). The standard anti-malarial used in this study showed the higher chemosupression. The phytochemical analysis also revealed the presence of the following bioactive components such as Saponins, Alkaloid and Flavonoids etc. The ethanol extract of Moringa oleifera seeds demonstrated a high anti-plasmodial activity against P. berghei in albino rats and affirms Moringa oleifera as a potential source of effective and safe herbal therapeutic agent in trado-medical practice for the treatment of fever, pain and malaria in Nigeria.

show abstract

Section: Resultscontrasting

confidence: 99%

Anti-plasmodial Effect of Moringa oleifera Seeds in Plasmodium berghei Infected Albino Rats

Ga¹,

Christian²

2020

Biochem Pharmacol

View full text Add to dashboard Cite

show abstract

“…Empat spesies Plasmodium yang menginfeksi manusia, yaitu P. falciparum (menyebabkan malaria tropika), P. malariae (menyebabkan malaria kuartana), P. vivax dan P. ovale (menyebabkan malaria tersiana). Namun dari keempat spesies tersebut, P. falciparum yang paling patogen dan bertanggungjawab untuk hampir semua kematian akibat malaria [5].…”

Section: Pendahuluanunclassified

Profil Fitokimia Ekstrak Etanol Bunga Kasumba Turate (Carthamus tinctorius L.) yang Berpotensi Sebagai Antimalaria

et al. 2019

View full text Add to dashboard Cite

Bunga Kasumba turate (Carthamus tinctorius Linn) dari suku Asteraceae merupakan tumbuhan obat tradisional etnis Sulawesi Selatan yang secara empiris digunakan dalam pengobatan campak. Ekstrak etanol dari kasumba turate memberikan peningkatan aktivitas imunoglobulin G (IgG) dan aktivitas imunoglobulin A (IgA) yang sangat signifikan. Penelitian menunjukkan ekstrak etanol bunga C. Tinctorius L memiliki potensi sebagai antimalaria secara in vitro dengan nilai IC50 sebesar 1,06 µg/ml. Sampel bunga C. tinctorius L. kering sebanyak 3 Kg dicacah hingga menjadi serbuk sebanyak 870 gram kemudian diekstraksi secara maserasi dengan menggunakan pelarut etanol 80%. Hasil maserasi dipekatkan dengan vacuum rotary evaporator pada suhu 50°C sehingga diperoleh ekstrak kental sebanyak 178,7gram. Profil fitokimia menunjukkan ekstrak etanol bunga C. tinctorius L. mengandung senyawa metabolit antara lain saponin, terpenoid, flavonoid, tanin dan antrakuinon.Kata kunci: Skrining, ekstrak, kasumba turate, obat tradisional, antimalaria

show abstract

“…Other studies have shown that Moringa possess a variety of properties including anti-diabetic, anti-inflammatory, anti-cancer [16], anti-ischemic [17] and even anti-plasmodial properties [15]. Of interest to the present study, Moringa has been shown to possess immune boosting properties [18], and potent curative/ suppressive effects on parasite burden in Plasmodium infection [8,19,20].…”

mentioning

confidence: 91%

“…For instance, a study performed by Dondee et al showed a dose dependent suppressive effect on parasite growth of up to 90% and an 80% reduction in parasite burden [19]. Olasehinde et al [20] also observed this suppressive effect in their study that employed both crude ethanolic and an n-hexane Moringa leaf extracts. They found that the crude ethanolic extract inhibited parasitaemia by 74.7 to 95.6% and n-hexane extract inhibited by 59.3 to 87.9%.…”

mentioning

confidence: 95%

Moringa oleifera treatment increases Tbet expression in CD4+ T cells and remediates immune defects of malnutrition in Plasmodium chabaudi-infected mice

Pilotos

Ibrahim

Mowa

et al. 2020

Malar J

View full text Add to dashboard Cite

Background: Malaria is a worldwide problem that affects millions of people yearly. In rural areas where anti-malarial drugs are not easily accessible, many people use herbal treatments, such as Moringa oleifera, to treat a variety of diseases and ailments including malaria. While Moringa is reported to possess potent and curative anti-malarial properties, previous studies have mostly been restricted to assessment of parasitaemia. In this study, the effect of Moringa on malaria immunity in a murine model was investigated.Methods: Using a high dose (60 mg/mouse) for a short time (7 days) or low dose Moringa (30 mg/mouse) for a longer time (3 weeks), cytokine production, and Tbet expression by effector CD4 + T cells (Teff ) were determined. Mice were also treated with Moringa after infection (curatively) or before infection (prophylactically) to determine the effect of the plant extract on parasitaemia and immunity. Given that Moringa also possess many nutritional benefits, the contribution of Moringa on malnourished malaria infected mice was determined. Malnutrition was induced by limiting access to food to only 4 h a day for 4 weeks, while control mice had unlimited access to mouse laboratory chow. All data was collected by flow cytometry and analysed using one-Way ANOVA or two tailed Student's t test.Results: Moringa-treated mice had increased numbers of effector CD4 + T cells accompanied by an increase in Tbet expression compared to control untreated mice. Mice that were treated with Moringa curatively also exhibited increased effector CD4 + T cell numbers, IFN-gamma and TNF secretion. Interestingly, the mice that were treated prophylactically had significantly higher Tbet expression. In the absence of adaptive immunity, high parasitaemia was observed in the RAG1 knockout mice. The food limited mice (malnourished) had reduced numbers of CD4 + T cells, TNF proportions, and significantly greater Tbet expression compared to the control group. Supplementation with Moringa in the limited group slightly restored CD4 + T cell activation, IL-2, and IL-10 production. Conclusions:Taken together, these data suggest that Moringa treatment leads to increased CD4 + T cell activation, Th1 differentiation and production of pro-inflammatory cytokines after malaria infection. Thus, Moringa may be immunologically useful in the treatment of malaria and malnutrition. Further investigations are required to identify the active components in Moringa.

show abstract

In vivo Antiplasmodial Activity of Crude Ethanolic and N-hexane Extracts of Moringa oleifera Leaves

Cited by 16 publications

References 22 publications

Anti-plasmodial Effect of Moringa oleifera Seeds in Plasmodium berghei Infected Albino Rats

Anti-plasmodial Effect of Moringa oleifera Seeds in Plasmodium berghei Infected Albino Rats

Profil Fitokimia Ekstrak Etanol Bunga Kasumba Turate (Carthamus tinctorius L.) yang Berpotensi Sebagai Antimalaria

Moringa oleifera treatment increases Tbet expression in CD4+ T cells and remediates immune defects of malnutrition in Plasmodium chabaudi-infected mice

Contact Info

Product

Resources

About