Isolation and characterization of biarylic structure-degrading yeasts: hydroxylation potential of dibenzofuran

Romero, María Cristina; Hammer, Elke; Cazau, María Cecilia; Arambarri, Ana María

doi:10.1016/s0269-7491(01)00290-1

Cited by 24 publications

(9 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…La cepa P. anomala, empleada como control positivo en los ensayos con única fuente de carbono, presentó el crecimiento más notorio en todos los hidrocarburos aromáticos analizados, lo cual es concordante con otros estudios que constatan su actividad degradadora de PAH como el dibenzofurano (Romero et al, 2002), así como naftaleno, fenantreno, crizeno y dibenzotiofeno (Pan et al, 2004). Se reporta su crecimiento en pireno.…”

Section: Ab469881unclassified

Aislamiento e identificación de levaduras degradadoras de hidrocarburos aromáticos, presentes en tanques de gasolina de vehículos urbanos

Delgadillo-Ordoñez

Posada-Suárez

Marcelo

et al. 2017

Rev. colomb. biotecnol.

View full text Add to dashboard Cite

Aislamiento e identificación de levaduras degradadoras de hidrocarburos aromáticos, presentes en tanques de gasolina de vehículos urbanosIsolation and identification of aromatic hydrocarbon degrading yeasts present in gasoline tanks of urbans vehicles DOI: 10.15446/rev.colomb.biote.v19n2.70278 RESUMENSe obtuvieron aislamientos de levaduras a partir de muestreos en tanques de combustible de vehículos urbanos, con el objeto de evaluar su potencial actividad de degradación de hidrocarburos aromáticos derivados del petróleo. Se realizaron ensayos de crecimiento en medio mínimo mineral sólido utilizando distintos hidrocarburos (benceno, tolueno, naftaleno, fenantreno, y pireno). Los aislamientos que presentaron crecimiento notorio en alguno de los hidrocarburos aromáticos policíclicos fueron identificados mediante secuenciación Sanger de los marcadores moleculares ITS1 e ITS2 del ARNr. Se obtuvieron 16 aislados de levaduras, de las cuales tres presentaron crecimiento conspicuo con hidrocarburos aromáti-cos como única fuente de carbono. Las cepas identificadas pertenecen al género Rhodotorula y corresponden a las especies Rhodotorula calyptogenae (99,8% de identidad) y Rhodotorula dairenensis (99,8% de identidad). Dichas cepas presentaron crecimiento en benceno, tolueno, naftaleno, fenantreno. En este estudio se reporta por primera vez la presencia de levaduras del género Rhodotorula que habitan ABSTRACTYeast isolates were obtained from fuel tanks of vehicles in order to assess their potential use in the degradation of aromatic hydrocarbons. Growth assays were performed in minimum mineral medium using different aromatic hydrocarbons (benzene, toluene, naphthalene, phenanthrene, and pyrene) as the sole carbon source. Isolates that showed growth in any of the tested polycyclic aromatic hydrocarbons were identified by Sanger sequencing of the ITS1 and ITS2 rDNA molecular markers. A total of 16 yeasts strains were isolated, and three showed remarkable growth in media with aromatic hydrocarbons as the sole carbon source. These strains belong to the genus Rhodotorula, and correspond to the species Rhodotorula calyptogenae (99,8% identity) and Rhodotorula dairenensis (99,8% identity). These strains grew in benzene, toluene, naphthalene, phenanthrene and pyrene. This study demonstrates for the first time that yeasts of the genus Rhodotorula inhabit pipelines and fuel tanks of vehicles and that remove aromatic hydrocarbons that are environmental pollutants. Our results suggest that these yeasts are potential candidates for aromatic hydrocarbon degradation as part of bioremediation strategies.

show abstract

Section: Ab469881unclassified

Aislamiento e identificación de levaduras degradadoras de hidrocarburos aromáticos, presentes en tanques de gasolina de vehículos urbanos

Delgadillo-Ordoñez

Posada-Suárez

Marcelo

et al. 2017

Rev. colomb. biotecnol.

View full text Add to dashboard Cite

show abstract

“…This enzymatic cyanide-removing property can be exploited for the detoxification of cyaniderich cassava wastewater and industrial residues. These residues currently cause serious environmental problems in many cassava flour producing plants in Nigeria, one of the largest producers worldwide, and in many African, Latin American and Asian countries (Romero et al, 2002). However, none has achieved complete detoxification (Blanshard et al, 1994) and in spite of the availability of several processing techniques, cyanide exposure from cassava diets prevails (Ernesto et al, 2000).…”

Section: Microorganismsmentioning

confidence: 99%

“…This enzymatic cyanide-removing property can be exploited for the detoxification of cyanide-rich cassava wastewater and industrial residues. These residues currently cause serious environmental problems in many cassava flour producing plants in Brazil, the largest producer worldwide, and in many African, Latin American and Asian countries (Romero et al, 2002), where cassava products are an important input for human diet. Manipueira is rich in potassium, nitrogen, magnesium, phosphorous, calcium, sulfur and iron, presenting a great potential as an agronomic fertilizer.…”

Section: Microorganismsmentioning

confidence: 99%

In vitro Degradation of Extracted Cassava Linamarin by Bacillus Species Isolated from cassava wastewater

Ogunyemi

Samuel

Buraimoh

et al. 2017

Egyptian Academic Journal of Biological Sciences, G. Microbiolo

View full text Add to dashboard Cite

Linamarin is the most abundant cyanoglucoside present in cassava cells and may generate the equivalent amount of hydrocyanic acid. This study was aimed to assess degradative capacities of Bacillus pumilus strain WOB3 and WOB7on linamarin. The test organisms for linamarase activity were identified on the basis of phenotype, biochemical properties and 16S rDNA gene sequencing as: Bacillus pumilus strain WOB3 KX774195 and Bacillus pumilus strain WOB7 KX774196. Growth studies showed that the strains grew in all the substrates tested. The doubling times of Bacillus pumilus strain WOB3 and Bacillus pumilus strain WOB7 were 8.25 d and 7.53 d on cassava effluent, 6.30 d and 5.78 d on supplemented cassava effluent, 8.66 d and 9.90 d on waste leachate and 6.30 d and 9.24 d on supplemented waste leachate respectively; with specific growth rates of 0.084 d-1 and 0.092 d-1 on cassava effluent, 0.11 d-1 and 0.12 d-1 on supplemented cassava effluent, 0.080 d-1 and 0.070 d-1 on waste leachate and 0.11 d-1 and 0.075 d-1 on supplemented waste leachate respectively. High-performance liquid chromatographic analysis studies revealed that linamarin degradation by the strains followed a linamarase pathway involving CO 2 and HCN as metabolic intermediates. Based on HPLC analysis, linamarin residual concentration at day 12 by the strains WOB3 and WOB7 was 26.73 mgL-1 (19.79 %) and 29.79 mgL-1 (21.92 %). These novel features make the bacteria suitable candidates for in-situ application on sites contaminated with cassava processing wastes.

show abstract

“…In this report 84% of hydrocarbon was removed from oil by composting it with C. catenulata. Other reports, mention the use C. krusei and C. rugosa, Pichia gilliermondii and Meyerozyma guil liermondii in biotransformation of pigments, lipase sources, degradation of polycyclic aromatic compounds and glycerol [39]- [43]. However, other species as D. hansenii are well known as food spoilage agents due to its salt and cold tolerance and no reports on its ability to degrade xenobiotic compounds have been reported.…”

Section: N-hexadecane Assimilation By Yeastsmentioning

confidence: 99%

The Evaluation of Bioremediation Potential of a Yeast Collection Isolated from Composting

Trama¹,

Fernandes²,

Labuto³

et al. 2014

AiM

View full text Add to dashboard Cite

The influence of xenobiotic compounds on environment and on living organisms has been reported as an imminent public health problem. Among them we can list the contamination by Alkanes present in petroleum, hydrocarbons and organic contaminant substances from industrial effluents. Also, heavy metals are of particular interest because of their persistence in the environment contaminating the food webs. Among the innovative solutions for treatment of contaminated water and soil is the use of biological materials like living or dead microorganisms. Yeasts exhibit the ability to adapt to extreme condition such as temperature, pH and levels of organic and inorganic contaminants that make them a potential material to be used to remediate contaminated environment application. The goal of this work was to search for yeast isolates capable to use n-hexadecane (alkane hydrocarbon) as a primary carbon source and for those able to tolerate high concentration of lead (Pb) within a collection of 90 isolates obtained from the São Paulo Zoo composting system. The isolated yeast strains were identified by mass spectrometry (MALDI-TOF-MS) and by sequencing of the ribosomal DNA (18S and D1/D2) conserved regions. We found that the collection bares 23 isolates capable of utilizing n-hexadecane and one which is able to tolerate high concentration of lead (Pb) with a high biosorption index compared to the reference yeast strains (BY4742, PE-2, CAT-1 and BG-1). These results confirm the initial hypothesis that the São Paulo Zoo composting is the source for diverse yeasts species with biotechnological application potential.

show abstract

Isolation and characterization of biarylic structure-degrading yeasts: hydroxylation potential of dibenzofuran

Cited by 24 publications

References 18 publications

Aislamiento e identificación de levaduras degradadoras de hidrocarburos aromáticos, presentes en tanques de gasolina de vehículos urbanos

Aislamiento e identificación de levaduras degradadoras de hidrocarburos aromáticos, presentes en tanques de gasolina de vehículos urbanos

In vitro Degradation of Extracted Cassava Linamarin by Bacillus Species Isolated from cassava wastewater

The Evaluation of Bioremediation Potential of a Yeast Collection Isolated from Composting

Contact Info

Product

Resources

About