Isolation and characterization of bacteriophage to control multidrug-resistant Pseudomonas aeruginosa planktonic cells and biofilm

Adnan, Muhammad Abdullah; Shah, Muhammad Rahman Ali; Jamal, Muhsin; Jalil, Fazal; Andleeb, Saadia; Nawaz, Muhammad Asif; Pervez, Sidra; Hussain, Tahir; Shah, Ismail; Imran, Muhammad; Kamil, Atif

doi:10.1016/j.biologicals.2019.10.003

Cited by 48 publications

(37 citation statements)

References 31 publications

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“…Literature has presented that bacteriophages or the synergistic action of bacteriophage and antibiotics constitute promising strategies against multidrug-resistant P. aeruginosa biofilm ( Akturk et al, 2019 ; Adnan et al, 2020 ). Given the ability of new lytic bacteriophages to disrupt and reduce the microbial load of endotracheal-associated biofilms in vitro , several aspects must be taken into consideration before the transfer of phage therapy to clinical practice.…”

Section: Discussionmentioning

confidence: 99%

“…The applicability of bacteriophages in medical devices has aroused interest in several areas, including infections related to the urinary tract ( Nzakizwanayo et al, 2015 ; Melo et al, 2016 ), venous catheters ( Lungren et al, 2014 ), and orthopedic prostheses ( Kaur et al, 2014 ; Barros et al, 2020 ). Recently, new lytic bacteriophages able to control multidrug-resistant P. aeruginosa planktonic cells and associated-biofilm forms were described ( Yuan et al, 2019 ; Adnan et al, 2020 ). Although promising, there are few reports on bacteriophage applicability to control biofilms associated with endotracheal tubes ( Aleshkin et al, 2016 ).…”

Section: Introductionmentioning

confidence: 99%

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Identification and Characterization of New Bacteriophages to Control Multidrug-Resistant Pseudomonas aeruginosa Biofilm on Endotracheal Tubes

et al. 2020

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Studies involving antimicrobial-coated endotracheal tubes are scarce, and new approaches to control multidrug-resistant Pseudomonas aeruginosa biofilm on these devices should be investigated. In this study, five new P. aeruginosa bacteriophages from domestic sewage were isolated. All of them belong to the order Caudovirales , Myoviridae family. They are pH and heat stable and produce 27 to 46 particles after a latent period of 30 min at 37°C. Their dsDNA genome (ranging from ∼62 to ∼65 kb) encodes 65 to 89 different putative proteins. They exhibit a broad lytic spectrum and infect 69.7% of the P. aeruginosa strains tested. All the bacteriophages were able to reduce the growth of P. aeruginosa strains in planktonic form. The bacteriophages were also able to reduce the biofilm viability rates and the metabolic activity of P. aeruginosa strains in a model of biofilms associated with endotracheal tubes. In addition, scanning electron microscopy micrographs showed disrupted biofilms and cell debris after treatment of bacteriophages, revealing remarkable biofilm reduction. The lytic activity on multidrug-resistant P. aeruginosa biofilm indicates that the isolated bacteriophages might be considered as good candidates for therapeutic studies and for the application of bacteriophage-encoded products.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Identification and Characterization of New Bacteriophages to Control Multidrug-Resistant Pseudomonas aeruginosa Biofilm on Endotracheal Tubes

et al. 2020

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“…The bacterial challenge test was performed according to the protocol previously reported with minor changes ( 42 ). Representative Salmonella strains were grown to an OD600 nm of 0.2, and were infected with phage SHWT1 at the optimal MOI.…”

Section: Methodsmentioning

confidence: 99%

“…The effects of phage SHWT1 on biofilm formation by Salmonella strains were tested referring to previous studies ( 42 , 43 ). Bacterial cultures with or without phage SHWT1 were dispensed in 96-well plates and cultured without shaking at 37°C for 24 or 48 h. Planktonic bacterial cells were removed and biofilms were stained with 200 μL crystal violet (0.1%, W/V) for 20 min.…”

Section: Methodsmentioning

confidence: 99%

Characterization of a Broad-Host-Range Lytic Phage SHWT1 Against Multidrug-Resistant Salmonella and Evaluation of Its Therapeutic Efficacy in vitro and in vivo

Tao

Zhang

et al. 2021

Front. Vet. Sci.

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Inappropriate use of antibiotics has accelerated to the emergence of multidrug-resistant bacteria, becoming a major health threat. Moreover, bacterial biofilms contribute to antibiotic resistance and prolonged infections. Bacteriophage (phage) therapy may provide an alternative strategy for controlling multidrug-resistant bacterial infections. In this study, a broad-host-range phage, SHWT1, with lytic activity against multidrug-resistant Salmonella was isolated, characterized and evaluated for the therapeutic efficacy in vitro and in vivo. Phage SHWT1 exhibited specific lytic activity against the prevalent Salmonella serovars, such as Salmonella Pullorum, Salmonella Gallinarum, Salmonella Enteritidis, and Salmonella Typhimurium. Morphological analysis showed that phage SHWT1 was a member of the family Siphoviridae and the order Caudovirales. Phage SHWT1 had a latent period of 5 min and burst size of ~150 plaque-forming units (PFUs)/cell. The phage was stable from pH 3-12 and 4–65°C. Phage SHWT1 also showed capacity to lyse Salmonella planktonic cells and inhibit the biofilm formation at optimal multiplicity of infection (MOI) of 0.001, 0.01, 0.1, and 100, respectively. In addition, phage SHWT1 was able to lyse intracellular Salmonella within macrophages. Genome sequencing and phylogenetic analyses revealed that SHWT1 was a lytic phage without toxin genes, virulence genes, antibiotic resistance genes, or significant genomic rearrangements. We found that phage SHWT1 could successfully protect mice against S. enteritidis and S. typhimurium infection. Elucidation of the characteristics and genome sequence of phage SHWT1 demonstrates that this phage is a potential therapeutic agent against the salmonellosis caused by multidrug-resistant Salmonella.

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“…An important point discussed in this study was that bacteriophage can degrade alginate polymers through enzymatic activities, and even it can destroy the 20-day biofilm formed by P. aeruginosa . Phage can also destroy biofilms indirectly by killing bacteria before attaching, or after colonizing the surface [ 41 ]. Another study examined the effect of PB1-like, phiKZ-like, and LUZ24-like phages against MDR P. aeruginosa under variable growth conditions; the results indicated that each phage alone was able to suppress planktonic and biofilm form of MDR isolates.…”

Section: Phage Therapy For Inhibition Of Mdr P Aeruginosa mentioning

confidence: 99%

Bacteriophage therapy against Pseudomonas aeruginosa biofilms: a review

Chegini

Khoshbayan

Moghadam

et al. 2020

Ann Clin Microbiol Antimicrob

160

101

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Multi-Drug Resistant (MDR) Pseudomonas aeruginosa is one of the most important bacterial pathogens that causes infection with a high mortality rate due to resistance to different antibiotics. This bacterium prompts extensive tissue damage with varying factors of virulence, and its biofilm production causes chronic and antibiotic-resistant infections. Therefore, due to the non-applicability of antibiotics for the destruction of P. aeruginosa biofilm, alternative approaches have been considered by researchers, and phage therapy is one of these new therapeutic solutions. Bacteriophages can be used to eradicate P. aeruginosa biofilm by destroying the extracellular matrix, increasing the permeability of antibiotics into the inner layer of biofilm, and inhibiting its formation by stopping the quorum-sensing activity. Furthermore, the combined use of bacteriophages and other compounds with anti-biofilm properties such as nanoparticles, enzymes, and natural products can be of more interest because they invade the biofilm by various mechanisms and can be more effective than the one used alone. On the other hand, the use of bacteriophages for biofilm destruction has some limitations such as limited host range, high-density biofilm, sub-populate phage resistance in biofilm, and inhibition of phage infection via quorum sensing in biofilm. Therefore, in this review, we specifically discuss the use of phage therapy for inhibition of P. aeruginosa biofilm in clinical and in vitro studies to identify different aspects of this treatment for broader use.

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Isolation and characterization of bacteriophage to control multidrug-resistant Pseudomonas aeruginosa planktonic cells and biofilm

Cited by 48 publications

References 31 publications

Identification and Characterization of New Bacteriophages to Control Multidrug-Resistant Pseudomonas aeruginosa Biofilm on Endotracheal Tubes

Identification and Characterization of New Bacteriophages to Control Multidrug-Resistant Pseudomonas aeruginosa Biofilm on Endotracheal Tubes

Characterization of a Broad-Host-Range Lytic Phage SHWT1 Against Multidrug-Resistant Salmonella and Evaluation of Its Therapeutic Efficacy in vitro and in vivo

Bacteriophage therapy against Pseudomonas aeruginosa biofilms: a review

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