Characterization of a Broad-Host-Range Lytic Phage SHWT1 Against Multidrug-Resistant Salmonella and Evaluation of Its Therapeutic Efficacy in vitro and in vivo

Tao, Chenglin; Yi, Zhengfei; Zhang, Yaodong; Wang, Yao; Hong, Zhu; Afayibo, Dossêh Jean Apôtre; Li, Tao; Tian, Mingxing; Qi, Jingjing; Ding, Chan; Gao, Song; Wang, Shaohui; Yu, Shuang

doi:10.3389/fvets.2021.683853

Cited by 22 publications

(13 citation statements)

References 63 publications

(99 reference statements)

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“…Host range was an essential factor to consider when choosing phages 45. Our results indicated that the isolated bacteriophages had a narrow range of activity. The results of the bacteriophage of Salmonella bacteria in the narrowness of their lytic activity agree with the results of Tao et al 2021 46 in that no lytic activity was observed against the other genera of bacteria. Moreover, results also indicated that pH as well as temperature can influence the efficacy of bacteriophage treatments of pathogenic microorganisms 47 .…”

Section: Bacteriophage Morphology Analysis Using Temsupporting

confidence: 89%

Isolation and Characterization of Lytic Phage against Salmonella Typhimurium

Alharbi

Alshaikh

2022

Biosci., Biotech. Res. Asia

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Significant prevalence of antibiotic resistance in Salmonella has been observed, causing global concern that it may lead to more severe health effects. Bacteriophages have emerged as an alternative treatment tool for managing bacterial infections, garnishing new attention. This study aimed to identify a Salmonella typhimurium-specific phage from chicken farms. The study verified the ability of lytic phage SAL 10 to stop the growth of bacteria. Furthermore, it involved conducting a series of phage analyses to verify their physical characteristics, such as temperature, pH, and host range. The Host ranges S. typhimurium of isolated phages against various strains were analyzed. Our results indicated that the isolated bacteriophages had a narrow range of activity. The phage was more stable at 37–50 °C and at pH 4–7. During the first 4 h of infection, phage SAL 10 inhibited the host bacterial growth. Following 24 h of incubation at 37 °C, we determined phage titration to be in the range of 103–108 PFU/mL in all experiments. Moreover, we determined the morphological properties of the phage using transmission electron microscopy, and the phage SAL 10 belonged to the order Caudovirales and family Siphoviridae. Results presented in this research show that SAL 10 phage can be used as a successful alternative to antibiotics.

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Section: Bacteriophage Morphology Analysis Using Temsupporting

confidence: 89%

Isolation and Characterization of Lytic Phage against Salmonella Typhimurium

Alharbi

Alshaikh

2022

Biosci., Biotech. Res. Asia

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“…Phages are generally species-specific or genus-specific ( Hyman and Abedon, 2010 ; Cazares et al, 2021 ). Broad-host range Salmonella phages have been reported in the literature ( Santos et al, 2011 ; Li, M. et al, 2020 ; Tao et al, 2021 ), but phages infecting bacteria from different genera are comparatively rare. It has been reported that phages SFP10 and KIT03 broke the boundary between Salmonella and Escherichia and infected both Salmonella enterica and E. coli O157:H7 ( Park et al, 2012 ; Pham-Khanh et al, 2019 ).…”

Section: Resultsmentioning

confidence: 99%

Bacteriophages Isolated From Turkeys Infecting Diverse Salmonella Serovars

Marchant

Thompson

et al. 2022

Front. Microbiol.

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Salmonella is one of the leading causes of foodborne illnesses worldwide. The rapid emergence of multidrug-resistant Salmonella strains has increased global concern for salmonellosis. Recent studies have shown that bacteriophages (phages) are novel and the most promising antibacterial agents for biocontrol in foods because phages specifically kill target bacteria without affecting other bacteria, do not alter organoleptic properties or nutritional quality of foods, and are safe and environmentally friendly. Due to the vast variation in Salmonella serotypes, large numbers of different and highly virulent Salmonella phages with broad host ranges are needed. This study isolated 14 Salmonella phages from turkey fecal and cecal samples. Six phages (Φ205, Φ206, Φ207, ΦEnt, ΦMont, and Φ13314) were selected for characterization. These phages were from all three families in the Caudovirales order. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) revealed that each phage had a unique structural protein profile. Each phage had a distinct host range. Φ207 and ΦEnt are both siphophages. They shared eight hosts, including seven different Salmonella serovars and one Shigella sonnei strain. These two phages showed different restriction banding patterns generated through EcoRI or HindIII digestion, but shared three bands from EcoRI digestion. ΦEnt displayed the broadest and very unusual host range infecting 11 Salmonella strains from nine serovars and three Shigella strains from two species, and thus was further characterized. The one-step growth curve revealed that ΦEnt had a short latent period (10 min) and relatively large burst size (100 PFU/infected cell). ΦEnt and its host showed better thermal stabilities in tryptic soy broth than in saline at 63 or 72°C. In the model food system (cucumber juice or beef broth), ΦEnt infection [regardless of the multiplicity of infections (MOIs) of 1, 10, and 100] resulted in more than 5-log10 reduction in Salmonella concentration within 4 or 5 h. Such high lytic activity combined with its remarkably broad and unusual host range and good thermal stability suggested that ΦEnt is a novel Salmonella phage with great potential to be used as an effective biocontrol agent against diverse Salmonella serovars in foods.

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“…8.0 × 10 −10 CFU −1 ·mL·min −1 and 7.0 × 10 −10 CFU −1 ·mL·min −1 , for phages ph001L and ph001T, respectively ( Figure 5 ). These results are of the same order of magnitude as the results reported by [ 85 ] (lytic phage fSPB adsorption rate on Salmonella serovar Paratyphi B, 4.7 × 10 −10 ) and [ 86 ] (adsorption rate of phage 1 = 2.2 × 10 −10 mL·min −1 and adsorption rate of phage 2 = 1.8 × 10 −10 mL·min −1 , onto Salmonella typhi ), one order of magnitude lower than results reported by [ 87 ] (phage PVP-SE1 adsorption rate on Salmonella enterica serovar Enteritidis strain S1400, 1.00 × 10 −9 mL·CFU −1 ·PFU −1 ·h −1 ) and [ 88 ] (phage SHWT1 adsorption rate on Salmonella pullorum SP01, (8.8 ± 0.5) × 10 −9 mL·min −1 ), and two orders of magnitude lower than the results reported by [ 89 ] (phage phi1 adsorption rate onto Salmonella enterica = 1.6 × 10 −8 mL·min −1 ). According to [ 84 ], the adsorption constants in some phages are close to the maximally possible values, viz.…”

Section: Discussionmentioning

confidence: 95%

An Edible Biopolymeric Microcapsular Wrapping Integrating Lytic Bacteriophage Particles for Salmonella enterica: Potential for Integration into Poultry Feed

et al. 2023

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This research work aimed at developing an edible biopolymeric microcapsular wrapping (EBMW) integrating lytic bacteriophage particles for Salmonella enterica, with potential application in poultry feed for biocontrol of that pathogen. This pathogen is known as one of the main microorganisms responsible for contamination in the food industry and in foodstuff. The current techniques for decontamination and pathogen control in the food industry can be very expensive, not very selective, and even outdated, such as the use of broad-spectrum antibiotics that end up selecting resistant bacteria. Hence, there is a need for new technologies for pathogen biocontrol. In this context, bacteriophage-based biocontrol appears as a potential alternative. As a cocktail, both phages were able to significantly reduce the bacterial load after 12 h of treatment, at either multiplicity of infection (MOI) 1 and 10, by 84.3% and 87.6%, respectively. Entrapment of the phage virions within the EBMW matrix did not exert any deleterious effect upon their lytic activity. The results obtained showed high promise for integration in poultry feed aiming at controlling Salmonella enterica, since the edible biopolymeric microcapsular wrapping integrating lytic bacteriophage particles developed was successful in maintaining lytic phage viability while fully stabilizing the phage particles.

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Characterization of a Broad-Host-Range Lytic Phage SHWT1 Against Multidrug-Resistant Salmonella and Evaluation of Its Therapeutic Efficacy in vitro and in vivo

Cited by 22 publications

References 63 publications

Isolation and Characterization of Lytic Phage against Salmonella Typhimurium

Isolation and Characterization of Lytic Phage against Salmonella Typhimurium

Bacteriophages Isolated From Turkeys Infecting Diverse Salmonella Serovars

An Edible Biopolymeric Microcapsular Wrapping Integrating Lytic Bacteriophage Particles for Salmonella enterica: Potential for Integration into Poultry Feed

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