Isolation and Characterization of an Ornithine‐Containing Lipid from <i>Paracoccus denitrificans</i>

Thiele, O. W.; Biswas, Clare Jamie; Hunneman, D. H.

doi:10.1111/j.1432-1033.1980.tb04497.x

Cited by 34 publications

(9 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, there are some remarkable exceptions from this rule. Our recent finding [6] that Paracoccus denitrificans contains phosphatidylcholine as a major phospholipid prompted us to look for phosphatidylcholine in other types of hydrogen bacteria.…”

Section: Occurrence Of Phosphatidylcholine In Hydrogen-oxidizing Bactmentioning

confidence: 99%

See 1 more Smart Citation

Occurrence of Phosphatidylcholine in Hydrogen‐Oxidizing Bacteria

Thiele

Oulevey

1981

European Journal of Biochemistry

Self Cite

View full text Add to dashboard Cite

15 strains of hydrogen‐oxidizing bacteria were grown heterotrophically, harvested during the stationary phase of growth, and analyzed for their principal phospholipids. All strainsndash;with the exception of Corynebacterium autotrophicum strain SA 32 and Pseudomonas pseudoflava–contained phosphatidylcholine as a major constituent. It is concluded that the presence of phosphatidy1choline is neither characteristic of a peculiar bacterial genus or family, nor is it absolutely correlated to the ability to oxidize hydrogen. The phosphatidylcholines of all strains contain C19 cyclopropane acid which is, in some strains, predominantly located at C‐2 position of the glycerol moiety.

show abstract

Section: Occurrence Of Phosphatidylcholine In Hydrogen-oxidizing Bactmentioning

confidence: 99%

“…The preparation of polar lipids from weighed portions of lyophilized bacteria was described previously [6]. Major phospholipids were fractionated by two-dimensional thinlayer chromatography of the polar lipid fraction [8].…”

Section: Quan T Ifica T Ion Of Phospholipidsmentioning

confidence: 99%

Occurrence of Phosphatidylcholine in Hydrogen‐Oxidizing Bacteria

Thiele

Oulevey

1981

European Journal of Biochemistry

Self Cite

View full text Add to dashboard Cite

show abstract

“…When P. denitrificans is grown in a complex medium, exponential-phase cells are lysozyme susceptible without further treatment (9,18 Previous studies of the outer membrane of P. denitrijicans have revealed that an ornithine-containing lipid is a major lipid species (21,26). A 3-hydroxy fatty acid is in amide linkage at the ot position and is esterified with a second nonhydroxy fatty acid.…”

mentioning

confidence: 99%

Increased outer membrane ornithine-containing lipid and lysozyme penetrability of Paracoccus denitrificans grown in a complex medium deficient in divalent cations

Wee

Wilkinson

1988

J Bacteriol

View full text Add to dashboard Cite

Paracoccus denitrificans grown in a complex medium was highly susceptible to lysozyme, in contrast to cells grown in a complex medium supplemented with Mg2+ and Ca2+ or in a succinate-salts medium. The complex medium was deficient in divalent cations needed for optimum outer membrane stability. The major change in molecular compositions of outer membranes isolated from cells grown under the different conditions was a higher ratio of ornithine-containing lipid to phospholipid in complex-medium-grown cells (0.63) than in cells grown in complex medium with Mg2' and Ca2+ (0.22) or in succinate-salts medium (0.14). We suggest that the dipolarionic ornithine-containing lipid is less dependent than acidic phospholipids on divalent cations for its incorporation into the outer membrane.Paracoccus denitrificans is a member of the a subdivision of the purple bacteria (28). This important group includes the purple non-sulfur bacteria, rhizobacteria, agrobacteria, rickettsiae, and Nitrobacter spp. This subdivision is composed predominantly of soil bacteria which tend to form intimate associations with eucaryotic cells and includes the endosymbiont ancestor of the mitochondrion (28). The organisms are thus of general biological interest. Although the outer membranes of these bacteria have been studied to some extent (1, 2), the main paradigm of outer membrane structure and function is still medically significant, mainly enteric, bacteria (8,14,17). Studies of the structure and function of the outer membrane of P. denitrificans are thus justified on the basis of the phylogeny of the organism.When P. denitrificans is grown in a complex medium, exponential-phase cells are lysozyme susceptible without further treatment (9,18 Iron-regulated proteins occur in the outer membrane, including high-Mr proteins induced upon iron deprivation (10) and a 23,000-Mr protein produced only in an iron-containing medium (22).In this paper we describe our investigations of outer membranes isolated from cells of the organism grown in complex medium, complex medium with Mg2' and Ca2+, and succinate-salts medium. We wanted to identify the changes in molecular composition associated with divalentcation deficiency and outer membrane stability. The major change under the different growth conditions was in the ratio of ornithine-containing lipid to phospholipid. P. denitrificans ATCC 13543 was grown to stationary phase with shaking at 30°C in complex medium (18), complex medium supplemented with 0.81 mM MgSO4 and 0.36 mM CaCI2, or succinate-salts medium (3). Our previously described procedure (16) for the isolation of cytoplasmic and outer membranes was used, with the following modifications. Cell envelopes were washed twice in 10 mM N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid (HEPES) buffer (pH 7.4) without MgCl2, and the sucrose gradients were 10 ml of 2.25 M, 12 ml of 1.44 M, and 10 ml of 0.77 M sucrose in 10 mM HEPES buffer (pH 7.4). Cell envelopes and cytoplasmic and outer membranes were made 1 mM in MgCI2 for recovery by ultracentrifugatio...

show abstract

“…Ten mg of total RNA from isolated testicular cells and from testes of rats of different ages were denatured in formamide/formaldehyde buffer, electrophoresed in a 2% formaldehyde/1.5% agarose gel and transferred to a nylon membrane (Hybond  -N; Amersham Pharmacia Biotech). After UV-crosslinking, membranes were hybridized with the appropriate [a- 32 and finally 1 cycle of 10 min at 727C. Amplified products were analyzed electrophoretically on a 1.5% agarose gel containing ethidium bromide.…”

Section: Northern Blottingmentioning

confidence: 99%

P. R. Giot, J. L'Helgouach, J.-L. Monnier, Préhistoire de la Bretagne, Rennes, Ouest France Université, 1979, 444 p. - P. R. Giot, J. Briard, L. Pape, Protohistoire de la Bretagne, Rennes, Ouest France Université, 1979, 444 p.

Guilaine¹

1980

Annales

View full text Add to dashboard Cite

Cellular distribution of translationally controlled tumor protein in rat and human testesIn a recent proteomic study we identified 53 spermatogonial proteins among which was the translationally controlled tumor protein (TCTP). This is a protein previously reported as being implicated in proliferation events in normal and tumoral tissues that had never previously been seen in the testis. The present study was aimed at establishing the complete cellular distribution of TCTP and its transcript and the ontogenetic expression of this gene within the testis. Using an immunohistochemistry technique, an intense TCTP signal was detected in gonocytes (the prespermatogonia) in the fetal rat testis and in spermatogonia within adult human and neonatal and adult rat testes. Meiotic spermatocytes and postmeiotic haploid spermatids were also strongly immunostained in a stage-dependent manner in human and rat testes. In addition, different levels of TCTP expression were also observed in the testicular somatic cells, with strong expression in Leydig cells and peritubular cells, and weak expression in Sertoli cells. Western and Northern blot analyses confirmed the presence of TCTP at all ages studied, with higher levels of RNA expression at 9 and 20 d postpartum, when spermatogonia and primary spermatocytes represent the highest proportion of germ cells: it was also confirmed that TCTP is present in all populations of isolated testicular cells. A transcript of 0.85 kb corresponding to TCTP, was expressed at all ages studied. This transcript was found to be expressed strongly in spermatogonia, somewhat less in isolated Leydig, resident macrophage, peritubular and Sertoli cells, weakly in the primary spermatocytes but not at all in spermatids. Interestingly, in the latter, a different transcript of 1.1 kb was present. The same 1.1 kb transcript appeared in testis extracts from 35 days postpartum onwards, corresponding to an age when spermatids accumulate within the tubules. Of note is that resident macrophages were found to express both the 0.85 and the 1.1 kb transcripts. We conclude that the strong expression of TCTP in spermatogonia makes it highly likely that the protein plays a significant role in spermatogenesis.Abbreviations: dpc, days postcoitum; dpp, days postpartum; TCTP, translationally controlled tumor protein * These authors contrubuted equally 880 Microdissection of the rat fetal gonadsPregnant Sprague-Dawley rats from 16.5 and 17.5 d postcoitum (dpc) were killed by cervical dislocation. The uterus was rapidly collected and the embryos separated Proteomics 2001,1,[880][881][882][883][884][885][886][887][888][889] TCTP in rat and human testes

show abstract

Isolation and Characterization of an Ornithine‐Containing Lipid from Paracoccus denitrificans

Cited by 34 publications

References 46 publications

Occurrence of Phosphatidylcholine in Hydrogen‐Oxidizing Bacteria

Occurrence of Phosphatidylcholine in Hydrogen‐Oxidizing Bacteria

Increased outer membrane ornithine-containing lipid and lysozyme penetrability of Paracoccus denitrificans grown in a complex medium deficient in divalent cations

P. R. Giot, J. L'Helgouach, J.-L. Monnier, Préhistoire de la Bretagne, Rennes, Ouest France Université, 1979, 444 p. - P. R. Giot, J. Briard, L. Pape, Protohistoire de la Bretagne, Rennes, Ouest France Université, 1979, 444 p.

Contact Info

Product

Resources

About