Isolation and Characterization of a Repetitive DNA Sequence from Leishmania infantum: Development of a Visceral Leishmaniasis Polymerase Chain Reaction

Piarroux, Renaud; Azaiez, R.; Lossi, Anne‐Marie; Reynier, Philippe; Muscatelli, Françoise; Gambarelli, F.; Fontés, Michel; Dumon, H.; Quilici, M

doi:10.4269/ajtmh.1993.49.364

Cited by 90 publications

(50 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For the detection of genomic Leishmania DNA, the PCR procedure described by Piarroux et al [30] was used with minor modifications. This procedure permitted the identification of a highly repetitive 100 bp sequence of L. infantum.…”

Section: Dna Extraction and Polymerase Chain Reaction (Pcr)mentioning

confidence: 99%

SDS-PAGE and Western blot of urinary proteins in dogs with leishmaniasis

et al. 2003

View full text Add to dashboard Cite

-Canine leishmaniasis is an endemic disease in the Mediterranean area caused by the protozoan Leishmania infantum, which usually produces renal failure. Sodium dodecyl sulphate polyacrylamide gel electrophoresis and Western blot using antibodies to IgG and IgA from dogs were carried out in the urine of 22 dogs with leishmaniasis diagnosed by ELISA and confirmed by PCR, and 20 healthy dogs. The results were compared to renal function laboratory tests and to those from a histopathological study of the kidneys from sick animals that died naturally or were euthanized. Five different bands with molecular weights ranging from 10 to 110 kDa were obtained from the electrophoresis of the urine of healthy dogs. 33.5% of total proteins corresponded to low molecular weight proteins and the other proteins had middle and high molecular weights. However, in the group with leishmaniasis, a maximum of 11 different bands with molecular weights ranging from 10 kDa to 150 kDa were displayed in the electrophoresis of the urine. The urine electrophoretic pattern in the sick dogs was classified as mixed (proteins with high and low molecular weights) because low molecular weight proteins made up 57.9% and the rest of the proteins had middle and high molecular weights. In Western blot, none of the healthy dogs showed excretion of IgG and/or IgA, whereas IgG and IgA were detected in the Western blot of urine of 68% and 55% respectively of dogs with leishmaniasis. The results obtained in the leishmaniasis group agreed with glomerular and tubular damage, which were confirmed by the histopathological findings. leishmaniasis / proteinuria / dog / SDS-PAGE / Western blot

show abstract

Section: Dna Extraction and Polymerase Chain Reaction (Pcr)mentioning

confidence: 99%

SDS-PAGE and Western blot of urinary proteins in dogs with leishmaniasis

et al. 2003

View full text Add to dashboard Cite

show abstract

“…The current state of the art diagnosis of L. siamensis infection relies on PCR and nucleotide sequencing. These techniques are more sensitive than others, 6,9,[28][29][30][31] and they can now be performed in most provincial and university hospitals in Thailand. Although use of traditional screening tests for this disease is being investigated, PCR could be used for survey and surveillance studies, including asymptomatic persons.…”

mentioning

confidence: 99%

Detection of Leishmania siamensis DNA in Saliva by Polymerase Chain Reaction

Phumee

Kraivichian²,

Chusri³

et al. 2013

The American Society of Tropical Medicine and Hygiene

View full text Add to dashboard Cite

Abstract. Polymerase chain reaction was used to detect Leishmania siamensis DNA from clinical samples collected from six leishmaniasis patients during 2011-2012. The samples used in this study came from bone marrow, blood, buffy coat, saliva, urine, and tissue biopsy specimens. Saliva was a good source for L. siamensis DNA by polymerase chain reaction. L. siamensis DNA was also found in saliva of an asymptomatic case-patient. Levels of L. siamensis DNA in saliva decreased until being undetectable after treatment. These levels could be used as a marker to evaluate efficacy of the treatment. A larger study is needed to evaluate this method as a screening and survey tool to study the silent background of Leishmania infection among the at-risk population.Leishmaniasis is a neglected tropical diseases caused by an obligate intracellular protozoa belonging to the genus Leishmania. The disease is transmitted to vertebrate hosts by infected female sand flies taking a blood meal.1 Leishmaniasis presents in three clinical forms; visceral, cutaneous, and mucocutaneous.2 Clinical presentation of leishmaniasis depends on the species of Leishmania and the immunity of the host. Detection and species identification of the parasites is essential for prognostic and therapeutic reasons and surveys.

show abstract

“…PCR with specific primers D1 and D2 for the L. donovani complex resulted in the amplification of kDNA of the L. (L.) donovani DD8 and L. (L.) infantum/chagasi PP75 reference strains, and bone marrow and liver biopsy from a patient from Chiapas with VL were amplified [16,21].…”

Section: Resultsmentioning

confidence: 99%

“…PCR of the L. braziliensis complex was done with the B1 and B2 primers [8]. PCR for L. donovani complex was done with the D1 and D2 primers [16]. PCR amplification conditions were performed as described previously [8,13,14,16,17].…”

Section: Polymerase Chain Reactionmentioning

confidence: 99%

Survey of Cutaneous Leishmaniasis in Mexico: Leishmania Species, Clinical Expressions and Risk Factors

Monroy-Ostria¹,

Sánchez-Tejeda²

2017

The Epidemiology and Ecology of Leishmaniasis

View full text Add to dashboard Cite

Leishmaniasis is caused by Leishmania sp., which is transmitted to human beings and reservoirs by phlebotomine sand flies, with worldwide prevalence of approximately 12 million cases with population at risk of approximately 350 million. Cutaneous leishmaniasis (CL) is the most widespread form, causing localized skin lesions (LCL), mucocutaneous leishmaniasis (MCL), or nodular lesions in diffused cutaneous leishmaniasis (DCL). American CL includes LCL and DCL caused by Leishmania mexicana complex and MCL caused by the Leishmania braziliensis complex. In Mexico, CL is distributed in three endemic areas, Gulf of Mexico, Pacific of Mexico, and Central Mexico. In order to monitor clinical outcome and adequately target treatment as well as epidemiologic studies, diagnostic kinetoplast DNA (kDNA), polymerase chain reaction (PCR), Southern and dot blotting, and ITS1 PCR-RFLP of Leishmania DNA were evaluated in samples and Leishmania isolates from patients with cutaneous ulcers from several endemic areas. In Mexico, LCL can be caused by the L. mexicana, L. braziliensis, or both complexes. DCL is caused by L.( L.) mexicana or Leishmania (L.) amazonensis and visceral leishmaniasis (VL) by Leishmania (L.) chagasi and L.( L.) mexicana in immunocompromised patients. The geographic range in which CL is endemic has increased due to urbanization, new settlements, and ecological, social, and educative conditions, which favors its permanence and transmission.

show abstract

Isolation and Characterization of a Repetitive DNA Sequence from Leishmania infantum: Development of a Visceral Leishmaniasis Polymerase Chain Reaction

Cited by 90 publications

References 0 publications

SDS-PAGE and Western blot of urinary proteins in dogs with leishmaniasis

SDS-PAGE and Western blot of urinary proteins in dogs with leishmaniasis

Detection of Leishmania siamensis DNA in Saliva by Polymerase Chain Reaction

Survey of Cutaneous Leishmaniasis in Mexico: Leishmania Species, Clinical Expressions and Risk Factors

Contact Info

Product

Resources

About