Isolation and characterization of a microorganism that produces biosurfactants in corn steep water

López-Prieto, Alejandro; Martínez-Padrón, Hadassa; Rodríguez‐López, Lorena; Cruz, J.M.

doi:10.1080/19476337.2019.1607909

Cited by 23 publications

(16 citation statements)

References 30 publications

(43 reference statements)

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“…Biosurfactant extracts from CSW are produced via spontaneous fermentation due to the steeping process of corn in the presence of SO 2 under acidic conditions and between temperatures of 45 • C and 52 • C [26]. Recently, it has been demonstrated that Bacillus strains are responsible for the production of biosurfactants in CSW under these extreme conditions [27]. Extracellular biosurfactants from CSW can be extracted using liquid-liquid (L-L) extraction with ethyl acetate or chloroform [28].…”

Section: Introductionmentioning

confidence: 99%

Fungistatic and Fungicidal Capacity of a Biosurfactant Extract Obtained from Corn Steep Water

López-Prieto

Vecino

Rodríguez‐López

et al. 2020

Foods

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Biosurfactants are surface-active compounds that are produced by microorganisms, which in addition to their surfactant capacity, can possess interesting antimicrobial activities that are used in their incorporation into the agrifood industry. In this work, the preservative capacity of a novel biosurfactant extract obtained from a residual stream of the corn-milling industry was evaluated against two different fungi (Aspergillus brasiliensis and Candida albicans) under different biosurfactant concentrations (0.33–0.99 mg/mL), temperatures (4–40 °C), and incubation times (5–11 days). All the assays started with the same concentration of fungi (2 × 106 CFU/mL). The results showed that temperature played an important role in the bactericidal and fungistatic effects of this biosurfactant extract. It was observed that at a low biosurfactant concentration (0.33 mg/mL) and low or high temperatures in the range tested, this biosurfactant extract possessed an important fungicidal effect (complete inhibition) on A. brasiliensis, while at intermediate temperatures, it achieved a fungistatic effect (50% of inhibition). Regarding C. albicans, it was observed that this strain was more resistant than A. brasiliens, although it was possible to achieve growth inhibitions of 76.3% at temperatures of 40 °C after 8 days of incubation with a biosurfactant concentration of 0.99 mg/mL. This work supports the possible application of biosurfactants extracted from corn steep water as preservatives and antimicrobial agents against fungal contaminations on agrifood products.

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Section: Introductionmentioning

confidence: 99%

Fungistatic and Fungicidal Capacity of a Biosurfactant Extract Obtained from Corn Steep Water

López-Prieto

Vecino

Rodríguez‐López

et al. 2020

Foods

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“…This is in consonance with the results previously reported, in which similar surfactant properties were observed in the same extracts BS1, BS2, BS3, and BS4, 25 in comparison with surfactin and other biosurfactants produced by Bacillus subtilis reported in the literature. 24 , 29 …”

Section: Resultsmentioning

confidence: 99%

Characterization and Cytotoxic Effect of Biosurfactants Obtained from Different Sources

et al. 2020

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In this work, five biosurfactant extracts, obtained from different sources, all of them with demonstrated antimicrobial properties, were characterized and subjected to a cytotoxic study using mouse fibroblast cells (NCTC clone 929). Biosurfactant extracts obtained directly from corn steep water (CSW) showed similar surfactant characteristics to those of the extracellular biosurfactant extract produced by Bacillus isolated from CSW and grown in tryptic soy broth, observing that they are amphoteric, consisting of viscous and yellowish liquid with no foaming capacity. Contrarily, cell-bound biosurfactant extracts produced from Lactobacillus pentosus or produced by Bacillus sp isolated from CSW are nonionic, consisting of a white powder with foaming capacity. All the biosurfactants possess a similar fatty acid composition. The cytotoxic test revealed that the extracts under evaluation, at a concentration of 1 g/L, were not cytotoxic for fibroblasts (fibroblast growth > 90%). The biosurfactant extract obtained from CSW with ethyl acetate, at 1 g/ L, showed the highest cytotoxic effect but above the cytotoxicity limit established by the UNE-EN-ISO10993-5. It is remarkable that the cell-bound biosurfactant produced by L. pentosus, at a concentration of 1 g/L, promoted the growth of the fibroblast up to 113%.

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Section: Resultsmentioning

confidence: 99%

“…Moreover, in this BS extract the presence of phospholipids compatible with phosphatidylethanolamine and phosphatidylcholine were also detected, these were separated and purified by Rodríguez-López et al (2020). In addition, the BS producer strain in CSL was isolated in a previous work, being characterized as an endospore-forming Bacillus strain, similar to Bacillus mycoides (López-Prieto et al, 2019a). Therefore, in this work the BS extracts contained in CSL were obtained following the methodology used in previous works (Rodríguez-López et al, 2020;Vecino et al, 2015) and identified applying different analytical methods described in the experimental section.…”

Section: Validation Of Predictive Equationmentioning

confidence: 99%

Towards more Ecofriendly Pesticides: Use of Biosurfactants Obtained from the Corn Milling Industry as Solubilizing Agent of Copper Oxychloride

López-Prieto

Moldes

Cruz

et al. 2020

J Surfact & Detergents

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Copper oxychloride (Cu-Oxy) is a widely used copper-based fungicide. A drawback of the product is its very low water solubility (around 0.2%) which limits its fungicide efficiency due to the low-penetrating capacity in the fungal mycelia. In this study, we have explored the use of biosurfactants (BS), surface-active compounds obtained by microbial production, to enhance the solubility of Cu-Oxy in agrochemical formulations. A BS extracted from corn steep liquor (CSL), a residue from the corn-milling industry, was evaluated as an additive to increase the solubility of Cu-Oxy in water. Different types of synthetic surfactants (Tween ® 80, sodium dodecyl sulfate (SDS) and hexadecyltrimethylammonium bromide (CTAB)) were included for comparison. Results showed that it was possible to dissolve around 90% of Cu-Oxy at concentration of 4 g L −1 in 20 min of contact time when the BS was added at the concentration of 20 g L −1. In contrast, the synthetic surfactants were only able to dissolve less than 0.5% of Cu-Oxy under the same conditions. In addition, a factorial design was carried out in order to evaluate the influence of BS concentration, Cu-Oxy concentration and contact time, on the solubilization of Cu-Oxy. Maximum percentage of solubilization (96.5%) was achieved at concentrations of 16.1 g L −1 of BS, 2 g L −1 of Cu-Oxy, and 20 min of contact time. These results allowed to conclude that BS extracted from CSL could be included in agrochemical formulations as solubilizing agents improving the stability of hydrophobic active principles and reducing the concentration needed of chemical pesticides, giving eco-friendlier formulations.

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Isolation and characterization of a microorganism that produces biosurfactants in corn steep water

Cited by 23 publications

References 30 publications

Fungistatic and Fungicidal Capacity of a Biosurfactant Extract Obtained from Corn Steep Water

Fungistatic and Fungicidal Capacity of a Biosurfactant Extract Obtained from Corn Steep Water

Characterization and Cytotoxic Effect of Biosurfactants Obtained from Different Sources

Towards more Ecofriendly Pesticides: Use of Biosurfactants Obtained from the Corn Milling Industry as Solubilizing Agent of Copper Oxychloride

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