Characterization and Cytotoxic Effect of Biosurfactants Obtained from Different Sources

Rodríguez‐López, Lorena; López-Prieto, Alejandro; López‐Álvarez, Miriam; Pérez-Davila, Sara; Serra, J.; González, P.; Cruz, J.M.

doi:10.1021/acsomega.0c04933

Cited by 25 publications

(17 citation statements)

References 66 publications

(128 reference statements)

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“…This value is considerably higher than those reported for commercial polysaccharides like xanthan and guar gum (approximately 0.15 g/L and 5 g/L, respectively) [29], suggesting a lower thermodynamic stability of the particles system [30]. Nevertheless, FucoPol reduced the surface tension of water from 72 mN/m to 54.6 mN/m at the CMC, a value that is within the range reported for other microbial biosurfactants (34-69 mN/m) [31][32][33]. Moreover, polymeric biosurfactants, despite not significantly lowering the water's surface tension, are generally more effective in the formation and stabilization of emulsions [31,34].…”

Section: Surface-active Propertiessupporting

confidence: 47%

Development of Olive Oil and α-Tocopherol Containing Emulsions Stabilized by FucoPol: Rheological and Textural Analyses

et al. 2022

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Biobased raw materials like natural polysaccharides are increasingly sought by the cosmetic industry for their valuable properties. Such biodegradable and usually non-cytotoxic biopolymers are commonly used in skin-care products as rheological modifiers, bioemulsifiers and/or bioactive ingredients. FucoPol is a natural polysaccharide with reported biocompatibility, emulsion-forming and stabilizing capacity, shear-thinning behavior and bioactivity (e.g., antioxidant capacity, wound healing ability) that potentiate its utilization in skin-care products. In this study, olive oil and α-tocopherol containing emulsions were stabilized with FucoPol. Although the presence of α-tocopherol negatively impacted the emulsions’ stability, it increased their emulsification index (EI). Moreover, FucoPol outperformed the commercial emulsifier Sepigel® 305, under the tested conditions, with higher EI and higher stability under storage for 30 days. The formulation of FucoPol-based emulsions with olive oil and α-tocopherol was studied by Response Surface Methodology (RSM) that allowed the definition of the ingredients’ content to attain high emulsification. The RSM model established that α-tocopherol concentration had no significant impact on the EI within the tested ranges, with optimal emulsification for FucoPol concentration in the range 0.7–1.2 wt.% and olive oil contents of 20–30 wt.%. Formulations with 25 wt.% olive oil and either 0.5 or 2.0 wt.% α-tocopherol were emulsified with 1.0 wt.% or 0.7 wt.% FucoPol, respectively, resulting in oil-in-water (O/W) emulsions. The emulsions had similar shear-thinning behavior, but the formulation with higher FucoPol content displayed higher apparent viscosity, higher consistency, as well as higher firmness, adhesiveness and cohesiveness, but lower spreadability. These findings show FucoPol’s high performance as an emulsifier for olive oil/α-tocopherol, which are supported by an effective impact on the physicochemical and structural characteristics of the emulsions. Hence, this natural polysaccharide is a potential alternative to other emulsifiers.

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Section: Surface-active Propertiessupporting

confidence: 47%

Development of Olive Oil and α-Tocopherol Containing Emulsions Stabilized by FucoPol: Rheological and Textural Analyses

et al. 2022

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“…In fact, at this time-point, the viability of HaCat cells was comparable to positive controls (p > 0.05), independently to the type of BS involved in the coating procedure (Supplementary Figure S3A). After 72 h of cells exposure to the eluates, cell viability slightly decreased to values ranging from 92% for SL18 to 78% for R89BS (Supplementary Figure S3B) but was always above the limit (70%) according to the ISO 10993-5 standard (Xian, 2009;Rodríguez-López et al, 2020).…”

Section: Cytotoxicity Of Bss-coated Silicone Discsmentioning

confidence: 99%

Inhibitory Effects of Lipopeptides and Glycolipids on C. albicans–Staphylococcus spp. Dual-Species Biofilms

et al. 2021

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Microbial biofilms strongly resist host immune responses and antimicrobial treatments and are frequently responsible for chronic infections in peri-implant tissues. Biosurfactants (BSs) have recently gained prominence as a new generation of anti-adhesive and antimicrobial agents with great biocompatibility and were recently suggested for coating implantable materials in order to improve their anti-biofilm properties. In this study, the anti-biofilm activity of lipopeptide AC7BS, rhamnolipid R89BS, and sophorolipid SL18 was evaluated against clinically relevant fungal/bacterial dual-species biofilms (Candida albicans, Staphylococcus aureus, Staphylococcus epidermidis) through quantitative and qualitative in vitro tests. C. albicans–S. aureus and C. albicans–S. epidermidis cultures were able to produce a dense biofilm on the surface of the polystyrene plates and on medical-grade silicone discs. All tested BSs demonstrated an effective inhibitory activity against dual-species biofilms formation in terms of total biomass, cell metabolic activity, microstructural architecture, and cell viability, up to 72 h on both these surfaces. In co-incubation conditions, in which BSs were tested in soluble form, rhamnolipid R89BS (0.05 mg/ml) was the most effective among the tested BSs against the formation of both dual-species biofilms, reducing on average 94 and 95% of biofilm biomass and metabolic activity at 72 h of incubation, respectively. Similarly, rhamnolipid R89BS silicone surface coating proved to be the most effective in inhibiting the formation of both dual-species biofilms, with average reductions of 93 and 90%, respectively. Scanning electron microscopy observations showed areas of treated surfaces that were free of microbial cells or in which thinner and less structured biofilms were present, compared to controls. The obtained results endorse the idea that coating of implant surfaces with BSs may be a promising strategy for the prevention of C. albicans–Staphylococcus spp. colonization on medical devices, and can potentially contribute to the reduction of the high economic efforts undertaken by healthcare systems for the treatment of these complex fungal–bacterial infections.

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“…The B. thailandensis biosurfactant outperforms synthetic surfactants such as SDS [ 40 ] and SLS [ 54 ], which display CMC values of 2.0–2.9 g/L, as well as the biosurfactant glycolipoprotein produced by a Bacillus sp. isolated from corn steep water, with a reported CMC value of around 1.81 ± 0.21 g/L [ 10 ].…”

Section: Resultsmentioning

confidence: 99%

“…DSM2567 (40 mN/m) [ 58 ], the lipopeptides produced by Bacillus sp. isolates (39.3 ± 0.6 and 37.7 ± 0.6 mN/m) [ 10 ], and the glycolipopeptide produced by Klebsiella sp. (40.36–69.09 mN/m) [ 35 ].…”

Section: Resultsmentioning

confidence: 99%

“…Biosurfactants are niche and present improved features, such as lower toxicity and better biodegradability and biocompatibility than their synthetic counterparts. According to several studies, biosurfactants are biocompatible with human cells and might find use as fibroblast growth factors [ 10 ]. Moreover, they can be produced using low-cost agro-industrial feedstocks, which makes the processes cost effective and environmentally sustainable [ 11 , 12 ].…”

Section: Introductionmentioning

confidence: 99%

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Characterization of the Thermostable Biosurfactant Produced by Burkholderia thailandensis DSM 13276

et al. 2022

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Biosurfactants synthesized by microorganisms represent safe and sustainable alternatives to the use of synthetic surfactants, due to their lower toxicity, better biodegradability and biocompatibility, and their production from low-cost feedstocks. In line with this, the present study describes the physical, chemical, and functional characterization of the biopolymer secreted by the bacterium Burkholderia thailandensis DSM 13276, envisaging its validation as a biosurfactant. The biopolymer was found to be a glycolipopeptide with carbohydrate and protein contents of 33.1 ± 6.4% and 23.0 ± 3.2%, respectively. Galactose, glucose, rhamnose, mannose, and glucuronic acid were detected in the carbohydrate moiety at a relative molar ratio of 4:3:2:2:1. It is a high-molecular-weight biopolymer (1.0 × 107 Da) with low polydispersity (1.66), and forms aqueous solutions with shear-thinning behavior, which remained after autoclaving. The biopolymer has demonstrated a good emulsion-stabilizing capacity towards different hydrophobic compounds, namely, benzene, almond oil, and sunflower oil. The emulsions prepared with the biosurfactant, as well as with its autoclaved solution, displayed high emulsification activity (>90% and ~50%, respectively). Moreover, the almond and sunflower oil emulsions stabilized with the biosurfactant were stable for up to 4 weeks, which further supports the potential of this novel biopolymer for utilization as a natural bioemulsifier.

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Characterization and Cytotoxic Effect of Biosurfactants Obtained from Different Sources

Cited by 25 publications

References 66 publications

Development of Olive Oil and α-Tocopherol Containing Emulsions Stabilized by FucoPol: Rheological and Textural Analyses

Development of Olive Oil and α-Tocopherol Containing Emulsions Stabilized by FucoPol: Rheological and Textural Analyses

Inhibitory Effects of Lipopeptides and Glycolipids on C. albicans–Staphylococcus spp. Dual-Species Biofilms

Characterization of the Thermostable Biosurfactant Produced by Burkholderia thailandensis DSM 13276

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