Isolation and characterization of a 29-kDa glycoprotein with antifungal activity from bulbs of Urginea indica

V, Ananda Deepak; Thippeswamy, G. B.; Shivakameshwari, M. N.; Salimath, Bharathi P.

doi:10.1016/j.bbrc.2003.10.056

Cited by 32 publications

(20 citation statements)

References 35 publications

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“…La présence des polyphénols dans les différents extraits des plantes étudiées est également rapportée par plusieurs auteurs Les effets antifongiques d'extrait aqueux d'Urginea maritima (L.) peuvent être attribués aux polyphénols de cette plante, mais certaines études ont démontré que l'activité antifongique d'Urginea maritima (L.) est liée à une glycoprotéine localisée dans la paroi cellulaire de l'ampoule, qui inhibe complètement la germination des spores et la croissance des hyphes de Fusarium oxysporum (Deepak et al, 2003 …”

Section: Resultats Et Discussion Dosage Des Polyphénolsunclassified

Activite Antifongique Des Extraits Aqueux De Calendula Officinalis L, Urginea Maritima (L.) Baker Et Chenopodium Ambrosioides L.

Daoudi

Bammou

Haloui

et al. 2017

ESJ

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Fungi are the cause of many human, animal and plant diseases. In the context of valorization of aromatic and medicinal plants of the Central Middle Atlas of Morocco, three plants were selected, based on the results of an ethnobotanical survey in this region, to evaluate their antifungal activity against five Fungi, namely Fusarium oxysporum sp. albedinis, Alternaria sp, Penicillium expansum, Aspergillus brasiliensis and Rhizopus stolonifer. A quantification of the total polyphenols was mesured out for the aqueous extracts of the studied plants Urginea maritima (L.) Baker of the Liliaceae family, Chenopodium ambrosoides L. of the Chenopodiaceae Family and Calendula officinalis L of the Asteraceae family, to choose the form in which the tests will be performed. According to the results, Aspergillus brasiliensis was found sensitive to the macerate of Urginea maritima (L.) Baker at a concentration of 10.71 mg / ml. The percent of inhibition (PI) of mycelial growth was 43.33%. At the same concentration, Fusarium oxysporum showed a slight sensitivity with PI = 19.25%. Furthermore the Chenopodium ambrosoides L decoctate was active against Aspergillus brasiliensis, Alternaria sp and Fusarium oxysporum with PI respectively equal to 41.11%,European Scientific Journal August 2017 edition Vol.13, No.24 ISSN: 1857 -7881 (Print) e -ISSN 1857-7431 48440.37% and 27.77%. However, the infusion of Calendula officinalis L showed no effect on the strains tested.Keywords: Antifungal activity, aqueous extracts, aromatic and medicinal plants, Central Middle Atlas, Morocco. ResumeLes champignons sont à l'origine de nombreuses maladies humaines, animales ou végétales. Dans le cadre de la valorisation des plantes aromatiques et médicinales de la région du Moyen Atlas central du Maroc, des plantes ont été choisies, en fonction des résultats d'une enquête ethnobotanique réalisée dans cette région, afin d'évaluer leur activité antifongique contre cinq champignons, à savoir Fusarium oxysporum sp. albedinis, Alternaria sp, Penicillium expansum, Aspergillus brasiliensis et Rhizopus stolonifer. Une quantification des polyphénols totaux a été effectuée sur des extraits aqueux des plantes étudiées à savoir Urginea maritima (L.) Baker famille des Liliacées, Chenopodium ambrosoides L. famille des Chenopodiacées et Calendula officinalis L de la famille des Asteracées, pour choisir la forme sous laquelle les tests seront réalisés. D'après les résultats obtenus, Aspergillus brasiliensis s'est révélé la souche la plus sensible vis-à-vis du macérât de Urginea maritima (L.) Baker, à une concentration de 10,71 mg/ml le pourcentage d'inhibition (PI) de la croissance mycélienne égale à 43,33 %, à la même concentration Fusarium oxysporum a montré une légère sensibilité avec PI = 19,25 %.Alors que, le décocté de Chenopodium ambrosoides L s'est monté active contre Aspergillus brasiliensis, Alternaria sp et Fusarium oxysporum avec des PI égaux à 41,11%, 40,37% et 27,77% respectivement. L'infusé de Calendula officinalis L n'a montré aucun pouvoir vis-à-vi...

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Section: Resultats Et Discussion Dosage Des Polyphénolsunclassified

Activite Antifongique Des Extraits Aqueux De Calendula Officinalis L, Urginea Maritima (L.) Baker Et Chenopodium Ambrosioides L.

Daoudi

Bammou

Haloui

et al. 2017

ESJ

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“…The results of the SDS−PAGE analysis of the two ‘peaks’ are shown in Figure 3A. A single major protein band was detected in ‘peak’ 1 with an apparent molecular mass of M r 29 kDa (23) (Figure 3A, lane P1). ‘Peak’ 2 also had the same M r 29 kDa band, which is expected since ‘peak’ 1 and ‘peak’ 2 were not completely separated.…”

Section: Resultsmentioning

confidence: 99%

“…The experiments described above showed that the M r 29 kDa U. indica protein exerts a potent antifungal action on the growth of fungi such as F. oxysporum and R. solani . To corroborate this finding using an alternative approach, we raised polyclonal antibodies (23) to the M r 29 kDa protein ('peak' 1) in rabbits to examine the effect of these antibodies on the antifungal activity of the protein. We reasoned that if the M r 29 kDa protein is truly responsible for the observed antifungal activity, it must be possible to block it by using antibodies specific to the M r 29 kDa protein.…”

Section: Resultsmentioning

confidence: 99%

Major Antifungal Activity from the Bulbs of Indian Squill Urginea indica Is a Chitinase

et al. 2006

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We have identified a chitinase with antifungal activity in the bulbs of the plant Urginea indica(Indian squill) and purified it about 26-fold. The purified preparation contained a Mr 29 kDa protein that was an active growth inhibitor of the fungal pathogens Fusarium oxysporum and Rhizoctonia solani in an in vitro assay. Amino acid sequence analysis of the Mr 29 kDa protein revealed it to be highly homologous to the family 19 glycoside hydrolases, which are known to possess chitinase activity. The U. indica chitinase lacked a cysteine-rich N-terminal domain (characteristic of class I chitinases) and contained a conserved motif indicative of the signature 1 of family 19 glycoside hydrolases. It shared a approximately 70% sequence identity with the 26 kDa endochitinase of Hordeum vulgare, a typical class II chitinase of family 19. The five cysteines in the partial sequence of the Mr 29 kDa chitinase were found to be identical in location to five of the seven cysteines present in the catalytic domain of the H. vulgare enzyme. The molecular weight, the lack of an N-terminal cysteine-rich sequence, and the striking identity to the H. vulgare endochitinase suggest that the Mr 29 kDa U. indica protein is a putative class II chitinase. The antifungal activity is presumably mediated through the chitinolytic activity of the Mr 29 kDa protein.

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“…In recent years, the functional significance of the carbohydrate moieties has been increasingly appreciated. Carbohydrates covalently attached to polypeptide chains can confer many functions to the glycoprotein, for example, resistance to proteolytic degradation and the transduction of information between cells are a few to mention (Deepak et al 2003).…”

Section: Discussionmentioning

confidence: 99%

Study on an antimicrobial protein produced by Paenibacillus polymyxa JSa-9 isolated from soil

Deng

Lü

et al. 2011

World J Microbiol Biotechnol

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Paenibacillus polymyxa strain JSa-9, a soil isolate showed in vitro inhibitory effects on several pathogens. A protein of about 71.9 kDa, isolated from a culture of JSa-9, exhibited broad-spectrum antibacterial and antifungal activities. The purification procedure consisted of ion-exchange chromatography on DEAE-Sephacel and Sephadex G-100 column chromatography. The purified protein was characterized to be a glycoprotein, which contained 53% of amino acids and 28% of carbohydrate. Its N-terminal sequence was determined as KCATIPVVIKHL and the amino acid composition showed no significant homology with any known antagonistic proteins from P. polymyxa. Because of the excellent antimicrobial activity, this protein may be a good prospect for food preservation and plant disease control.

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Isolation and characterization of a 29-kDa glycoprotein with antifungal activity from bulbs of Urginea indica

Cited by 32 publications

References 35 publications

Activite Antifongique Des Extraits Aqueux De Calendula Officinalis L, Urginea Maritima (L.) Baker Et Chenopodium Ambrosioides L.

Activite Antifongique Des Extraits Aqueux De Calendula Officinalis L, Urginea Maritima (L.) Baker Et Chenopodium Ambrosioides L.

Major Antifungal Activity from the Bulbs of Indian Squill Urginea indica Is a Chitinase

Study on an antimicrobial protein produced by Paenibacillus polymyxa JSa-9 isolated from soil

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