ISOLASI DAN IDENTIFIKASI BAKTERI ASAM LAKTAT PENGHASIL INHIBITOR ENZIM HMG-KoA REDUKTASE DARI BEKASAM SEBAGAI AGEN PEREDUKSI  KOLESTEROL

Rinto, Rinto; Dewanti, Ratih; Yasni, Sedarnawati; Suhartono, Maggy Thenawidjaja

doi:10.22146/agritech.9342

Cited by 7 publications

(7 citation statements)

References 3 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…An enzyme that plays a major rule in cholesterol biosynthesis is a HMG-CoA (Hydroxymethylglutarate-CoA) reductase. Lactobacillus casei Shirota strain fermented milk containing lactic acid bacteria that produced statins compound that could inhibit the HMG-CoA reductase enzyme so the formation of cholesterol was restrained [5].…”

Section: Results and Discussion Decreasing Total Cholesterol Levels VImentioning

confidence: 99%

Therapy of Lactobacillus casei Shirota strain Fermented Milk to Rats with High-Cholesterol Diet toward Cholesterol Levels and SOD Activity

Rohma

Mahdi

Aulanni’am

2017

J. Pure App. Chem. Res.

View full text Add to dashboard Cite

This study aimed to determine the fermented milk of Lactobacillus casei Shirota strain potential on cholesterol levels, SOD activity, LPL enzyme activity, and cardiac histopathology. This study used 25 rats (Rattus novergicus) Wistar strain aged 2-3 months, weighing 150-200 grams. These rats were divided into 5 groups: negative control group, positive control group with high-cholesterol diet for 21 days, and therapy group with 1.0 ; 1.5 ; 2.0 mL/rat/day of fermented milk for 14 days. The analysis methods used in this study are One Way ANOVA and Tukey. The results showed that the fermented milk of Lactobacillus casei Shirota strain had a strong effect on blood cholesterol levels and increasing the SOD activity. This study also showed that the fermented milk of Lactobacillus casei with 2.0 mL/rat/day is the most effective dose to decrease the total cholesterol and increase the SOD activity.Keywords: cholesterol, fermented milk of Lactobacillus casei Shirota strain, total cholesterol, SOD INTRODUCTIONCholesterol is a steroid alcohol on the class of lipids (fats) which play a part as a source of calories. In addition, cholesterol is a component of fat found in the blood of animals and humans that produced by the liver and used as an energy producer. It is also the wallforming cells in the body and as a basic ingredient of the steroid hormones formation in the body. Cholesterol is the precursor of all steroids, such as corticosteroids, sex hormones, bile acids, and vitamin D.Consuming lots of high-cholesterol diet can lead to the increasing of cholesterol levels. It causes hypercholesterolemia, atherosclerosis in the arteries, and blood vessels constriction in the heart, brain, kidneys, and eyes when it reaches beyond the normal limits. Hypercholesterolemia is a condition when the blood cholesterol level exceeds the normal value of 200 mg/dL. In the body, cholesterol found in lipoproteins, such as chylomicrons, which contains triglycerides, VLDL (very low density lipoprotein) to transport triglycerides. The triglycerides formed by the heart, LDL (low density lipoprotein) or beta-lipoprotein consisting of 25% protein and 40% cholesterol and HDL (high density lipoprotein) or alpha lipoprotein contain 50% protein and 20% cholesterol [1].

show abstract

Section: Results and Discussion Decreasing Total Cholesterol Levels VImentioning

confidence: 99%

Therapy of Lactobacillus casei Shirota strain Fermented Milk to Rats with High-Cholesterol Diet toward Cholesterol Levels and SOD Activity

Rohma

Mahdi

Aulanni’am

2017

J. Pure App. Chem. Res.

View full text Add to dashboard Cite

show abstract

“…Komponen bioaktif merupakan komponen kimia yang sangat baik untuk kesehatan manusia, yaitu dapat digunakan sebagai pencegahan terhadap penyakit hipertensi, kolesterol tinggi dan bakteri patogen. Rinto et al, (2015), berhasil mengisolasi Lactobacillus acidophilus dari bekasam, yang merupakan bakteri asam laktat penghasil komponen bioaktif lovastatin dan peptida bioaktif penghambat sintetis kolesterol. Lovastatin merupakan obat golongan statin yang dapat menghambat aktivitas enzim HMGR, sehingga sintesis kolesterol dalam hati akan terhambat.…”

Section: Abstrakunclassified

“…Parameter pengamatan pada penelitian ini yaitu pH AOAC (1995), n-amino Sudarmaji et al, (1997), peptida (Rinto et al, (2017a(Rinto et al, ( dan 2017b, lovastatin (Rinto et al, (2015).…”

Section: Parameter Pengamatanunclassified

“…Produksi lovastatin pada bekasam karena adanya pertumbuhan bakteri jenis Lactobacillus acidophilus. Hal ini lebih diperkuat oleh pernyataan dari Rinto et al, (2015), yang menyatakan bahwa Lactobacillus acidophilus asal bekasam diketahui dapat memproduksi lovastatin.…”

Section: Lovastatinunclassified

“…Pada penelitian yang dilakukan oleh Rinto et al, (2015), kandungan lovastatin pada bekasam ikan gabus dan ikan seluang berkisar antara 73-88 ppm. Sedangkan pada penelitian ini, terjadi penurunan terhadap kandungan lovastatin bekasam.…”

Section: Gambar 42 Produksi Lovastatin Bekasamunclassified

See 2 more Smart Citations

Pengaruh Waktu Penyangraian Beras terhadap Komponen Bioaktif pada Bekasam Ikan Nila (Oreochromis niloticus)

Rinto

Widiastuti

Lestari³

et al. 2021

FishtecH

Self Cite

View full text Add to dashboard Cite

The objective of this research was to determine the effect of rice roasting time on bioactive compounds in bekasam from tilapia. This research used a Randomized block Design with four treatments. The treatments were the production of bekasam used rice (as a control) and rice with roasting time (0, 5, 10, 15 minutes). The parameters observed were acidity level (pH), lovastatin, peptide, and n-amino contents. The results of this study indicate that, roasting rice had a significant effect on the lovastatin content (54.35-57.73 ppm), where the highest lovastatin content was bekasam was produced by rice treatment (control). Rice roasting gived no significant affect to the pH value (6.12-6.35), peptide (9.24%-14.28%), and n-amino (0.014%-0.028%). Based on results of the research, fermentation bekasam process of tilapia fish with rice and rice roasting in the vacuum packaging has not been perfect.

show abstract

The effect of degradation time in the simulated gastric fluid for bioactive compounds from bekasam

Rinto

Lestari

Baehaki

et al. 2020

IOP Conf. Ser.: Earth Environ. Sci.

View full text Add to dashboard Cite

Bekasam contains bioactive compounds (lovastatin and peptide) that has function as anticholesterol. This research was aimed to determine the effect of pH and degradation time to lovastatin, peptides, and dissolved protein in bekasam seluang extract (Rasbora sp.). This study used a laboratory experimental method with 4 levels of treatment (degradation in simulation gastric fluid pH 2, pH 3 pH 4 and control without simulated gastric fluid). The degradation results were observed every the 2nd hours, the 4th hours, and the 6th hours. Each treatment level repeated 3 times. The stages of the study consisted of making bekasam, extracting of bekasam, calculating the yield of bekasam extract and testing pure bekasam extract in simulated gastric fluid. The analysis was carried out using a descriptive method, which is to describe each parameter obtained. The parameters observed are analysis of lovastatin level, dissolved protein levels, and peptide levels. The results showed that the levels of lovastatin, dissolved proteins and peptides were still detectable after have degraded in simulation gastric fluid an acidity level of pH 2, pH 3 and pH 4 for 2nd to 6th hours.

show abstract

ISOLASI DAN IDENTIFIKASI BAKTERI ASAM LAKTAT PENGHASIL INHIBITOR ENZIM HMG-KoA REDUKTASE DARI BEKASAM SEBAGAI AGEN PEREDUKSI KOLESTEROL

Cited by 7 publications

References 3 publications

Therapy of Lactobacillus casei Shirota strain Fermented Milk to Rats with High-Cholesterol Diet toward Cholesterol Levels and SOD Activity

Therapy of Lactobacillus casei Shirota strain Fermented Milk to Rats with High-Cholesterol Diet toward Cholesterol Levels and SOD Activity

Pengaruh Waktu Penyangraian Beras terhadap Komponen Bioaktif pada Bekasam Ikan Nila (Oreochromis niloticus)

The effect of degradation time in the simulated gastric fluid for bioactive compounds from bekasam

Contact Info

Product

Resources

About