Isoelectric Focusing and Two-Dimensional Gel Electrophoresis in Plasma and Cerebrospinal Fluid from Patients with Dementia

Alafuzoff, Irina; Adolfsson, Rolf; Bucht, Gösta; Jellum, Egil; Mehta, P. D.; Winblad, Bengt

doi:10.1159/000116023

Cited by 12 publications

(8 citation statements)

References 16 publications

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“…Also, the number and type of patients studied have varied. In the present study, again, no disease-specific alterations in 2-D protein maps between the dementia and control groups could be observed, which is in agreement with earlier reports (24)(25)(26). Nevertheless, although no definitive markers are available, recent studies suggest that there may be quantitative differences in the amount of certain proteins in the CSF between AD and age-matched control patients (27-3 1).…”

Section: Resultssupporting

confidence: 93%

Altered blood-brain-barrier function in Alzheimer's disease?

Mattila

Pirttilä

Blennow

et al. 2009

Acta Neurologica Scandinavica

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Alzheimer's disease (AD) and vascular dementia (VD) are the two most common causes of dementia. As yet, no definitive biological antemortem marker has been established for differential diagnosis of AD or VD. In this study, proteins of cerebrospinal fluid (CSF) from AD, VD and control patients were analyzed by two‐dimensional (2‐D) electrophoresis with immobilized pH gradients in the first dimension. No specific changes for AD or VD could be detected in the 2‐D CSF patterns. However, a spot of haptoglobin alpha‐1 chains (13.5 kDa; approximate pI 4.6) was found to be present in the majority of 2‐D CSF maps from the dementia cases, suggesting a high‐molecular‐weight transudate type of alteration in the blood‐brain barrier with considerable frequency in AD.

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Section: Resultssupporting

confidence: 93%

Altered blood-brain-barrier function in Alzheimer's disease?

Mattila

Pirttilä

Blennow

et al. 2009

Acta Neurologica Scandinavica

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“…Several 2-DE studies have been performed without successful identification of AD-specific changes in the CSF proteome (58)(59)(60)(61). In the early 1990's, we found that an isoform of haptoglobin-alpha)1 chains was present in the majority of 2-D maps in AD vs. age-matched controls, indicating a possible altered blood-brain-barrier function in AD (62).…”

Section: -De Studies In Admentioning

confidence: 84%

Problems Associated with Biological Markers of Alzheimer’s Disease

Frey¹,

Mattila

Korolainen

et al. 2005

Neurochem Res

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The etiopathogenesis of Alzheimer's disease (AD) is still unclear, although clinical diagnostic criteria exist and the neuropathology of AD has been studied in great detail during the last 20 years. The present study addresses certain problems in the search for biological markers for the diagnosis, as well as in the follow-up of the course of AD and its differential diagnosis and reports some of our own observations in comparison with other studies. These include protein, genetic and neuroimaging markers. The definitions of biological markers and search strategies are also discussed.

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“…Although the occurrence of CNS specific OCB in the CSF of patients with Alzheimer's disease was first reported in 1980 [16] this and subsequent reports have only evaluated relatively small numbers of patients [1,5,10] [3,4,7,8]. The presence of OCB in primary neurodegenerative dementia is generally considered to be a rarity such that their absence is supportive of diagnosis, specifically for example of probable AD [13].…”

Section: Introductionmentioning

confidence: 99%