Isocitrate dehydrogenases from liver ofSqualus acanthias (spiny dogfish) and citrate formation by isolated mitochondria

Saxrud, Kristine M.; Lambeth, David O.; Anderson, Paul N.

doi:10.1002/(sici)1097-010x(19960415)274:6<334::aid-jez2>3.0.co;2-p

Cited by 3 publications

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“…In the presence of a divalent cation (Mn 2+ or Mg 2+ ) both forms catalyze the oxidative decarboxilation of isocitric acid to α-ketoglutarate. The cytosolic NADP-dependent isocitrate dehydrogenase (IDHP) function is to provide NADPH for lipid biosynthesis, and α-ketoglutarate for glutamate and glutamine formation (Saxrud et al, 1996). Multiple forms of IDHP encoded by unlinked gene loci have been described in fish (Allendorf et al, 1977;Van-Beneden et al, 1981;Perdices et al, 1996;Doadrio & Carmona, 1998;Chiari & Sodré, 1999;Kohlmann & Kersten, 1999;Renesto et al, 2000).…”

Section: Introductionmentioning

confidence: 99%

NADP-dependent isocitrate dehydrogenase of Astyanax scabripinnis (Pisces, Characidae) from three altitudes at Grande Stream, Campos do Jordão, SP

Munin

Schwantes

et al. 2004

Braz. J. Biol.

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Electrokinetic, thermic, and kinetic properties of products of NADP-dependent isocitrate dehydrogenase (IDHP; EC 1.1.1.42) loci of Astyanax scabripinnis (Pisces, Characidae) collected at three different altitudes (700 m, 1,800 m, and 1,920 m) of Grande Stream at Campos do Jordão, State of São Paulo, Brazil, were analyzed. Two IDHP bidirectionally divergent loci, a single skeletal muscle, the IDHP-A*, and a single liver IDHP-B*, both polymorphic, were detected in the three different altitude populations. The variant allele *128 at the IDHP-A* locus, had its highest frequency detected in the 1,920 m population (0.494). Among the nine variant alleles detected at the IDHP-B* locus (*37, *57, *69, *79, *85, *114, *119, *124, and *140), the *37 and 79 were detected only in 1,800 m population. Chi-square values showed that only the 700 m population is not in Hardy-Weinberg equilibrium for the IDHP-A* locus, while for the IDHP-B* locus, no population is. Homogeneity Chi2 test indicated that the populations are significantly different in their A and B phenotype frequencies. Wright's FST mean value (0.036 and 0.32, IDHP-A* and IDHP-B*, respectively) was 0.178 for the three altitude populations which means that 82% of total genetic diversity was found among individuals of each one of the populations. Stability at environmental temperatures (16 degrees to 21 degrees C), and apparent Km and Vmax values of each A-phenotype skeletal muscle crude extract suggest different roles of A-isoforms during the increased lipogenesis that occurs in fish at low temperatures.

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