Isobutanol production from<scp>d</scp>-xylose by recombinant<i>Saccharomyces cerevisiae</i>

Brat, Dawid; Boles, Eckhard

doi:10.1111/1567-1364.12028

Cited by 56 publications

(36 citation statements)

References 11 publications

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“…However, prior studies reported contrary results to this expectation. No meaningful improvements in the production of 2,3-butanediol [142] and isobutanol [143] which are derived from pyruvate have been reported under xylose conditions. A notable difference between the enhanced lactic acid production and the latter ineffectual production of 2,3-butanediol and isobutanol is the order of metabolic engineering efforts.…”

Section: Production Of Advanced Biofuels and Chemicals From Xylose Bymentioning

confidence: 99%

Production of fuels and chemicals from xylose by engineered Saccharomyces cerevisiae: a review and perspective

2017

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Efficient xylose utilization is one of the most important pre-requisites for developing an economic microbial conversion process of terrestrial lignocellulosic biomass into biofuels and biochemicals. A robust ethanol producing yeast Saccharomyces cerevisiae has been engineered with heterologous xylose assimilation pathways. A two-step oxidoreductase pathway consisting of NAD(P)H-linked xylose reductase and NAD+-linked xylitol dehydrogenase, and one-step isomerase pathway using xylose isomerase have been employed to enable xylose assimilation in engineered S. cerevisiae. However, the resulting engineered yeast exhibited inefficient and slow xylose fermentation. In order to improve the yield and productivity of xylose fermentation, expression levels of xylose assimilation pathway enzymes and their kinetic properties have been optimized, and additional optimizations of endogenous or heterologous metabolisms have been achieved. These efforts have led to the development of engineered yeast strains ready for the commercialization of cellulosic bioethanol. Interestingly, xylose metabolism by engineered yeast was preferably respiratory rather than fermentative as in glucose metabolism, suggesting that xylose can serve as a desirable carbon source capable of bypassing metabolic barriers exerted by glucose repression. Accordingly, engineered yeasts showed superior production of valuable metabolites derived from cytosolic acetyl-CoA and pyruvate, such as 1-hexadecanol and lactic acid, when the xylose assimilation pathway and target synthetic pathways were optimized in an adequate manner. While xylose has been regarded as a sugar to be utilized because it is present in cellulosic hydrolysates, potential benefits of using xylose instead of glucose for yeast-based biotechnological processes need to be realized.

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Section: Production Of Advanced Biofuels and Chemicals From Xylose Bymentioning

confidence: 99%

Production of fuels and chemicals from xylose by engineered Saccharomyces cerevisiae: a review and perspective

2017

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“…However, these processes are highly dependent on the purity of the waste stream and face several challenges before being scaled up. Biological approaches being developed include production of PHB (polyhydroxybutyrate), a feedstock for the production of bioplastics (Garcez Lopes et al 2011); and biofuels, such as ethanol, isobutanol and triacylglycerol (TAG) (Brat & Boles 2013;Kurosawa et al 2013;Q. Li et al 2008).…”

Section: Introductionmentioning

confidence: 99%

Utilization of biodiesel-derived glycerol or xylose for increased growth and lipid production by indigenous microalgae

Leite

Paranjape

Abdelaziz

et al. 2015

Bioresource Technology

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The use of industrial wastes rich in mineral nutrients and carbon sources to increase the final microalgal biomass and lipid yield at a low cost is an important strategy to make algal biofuel technology viable. Using strains from the microalgal collection of the Université de Montréal, this report shows for the first time that microalgal strains can be grown on xylose, the major carbon source found in wastewater streams from pulp and paper industries, with an increase in growth rate of 2.8 fold in comparison to photoautotrophic growth, reaching up to µ=1.1/day. On glycerol, growth rates reached as high as µ=1.52/day. Lipid productivity increased up to 370% on glycerol and 180% on xylose for the strain LB1H10, showing the suitability of this strain for further development for biofuels production through mixotrophic cultivation.Final Version available online at: http://dx

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“…We focused our attention on the strategy which takes advantage of ketoacids as intermediates in amino acids biosynthesis and degradation metabolism to produce fusel alcohols in the yeast S. cerevisiae . While the pathway to isoketovalerate was better elucidated and recently successfully exploited for isobutanol formation in S. cerevisiae [13-17], butanol production from ketovalerate was never experimentally measured.…”

Section: Introductionmentioning

confidence: 99%

A novel pathway to produce butanol and isobutanol in Saccharomyces cerevisiae

Branduardi

Longo

Berterame

et al. 2013

Biotechnol Biofuels

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BackgroundThe sustainable production of biofuels remains one of the major issues of the upcoming years. Among the number of most desirable molecules to be produced, butanol and isobutanol deserve a prominent place. They have superior liquid-fuel features in respect to ethanol. Particularly, butanol has similar properties to gasoline and thus it has the potential to be used as a substitute for gasoline in currently running engines. Clostridia are recognized as natural and good butanol producers and are employed in the industrial-scale production of solvents. Due to their complex metabolic characteristics and to the difficulty of performing genetic manipulations, in recent years the Clostridia butanol pathway was expressed in other microorganisms such as Escherichia coli and Saccharomyces cerevisiae, but in yeast the obtained results were not so promising. An alternative way for producing fusel alcohol is to exploit the degradation pathway of aminoacids released from protein hydrolysis, where proteins derive from exhausted microbial biomasses at the end of the fermentation processes.ResultsIt is known that wine yeasts can, at the end of the fermentation process, accumulate fusel alcohols, and butanol is among them. Despite it was quite obvious to correlate said production with aminoacid degradation, a putative native pathway was never proposed. Starting from literature data and combining information about different organisms, here we demonstrate how glycine can be the substrate for butanol and isobutanol production, individuating at least one gene encoding for the necessary activities leading to butanol accumulation. During a kinetic of growth using glycine as substrate, butanol and isobutanol accumulate in the medium up to 92 and 58 mg/L, respectively.ConclusionsHere for the first time we demonstrate an alternative metabolic pathway for butanol and isobutanol production in the yeast S. cerevisiae, using glycine as a substrate. Doors are now opened for a number of optimizations, also considering that starting from an aminoacid mixture as a side stream process, a fusel alcohol blend can be generated.

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Isobutanol production fromd-xylose by recombinantSaccharomyces cerevisiae

Cited by 56 publications

References 11 publications

Production of fuels and chemicals from xylose by engineered Saccharomyces cerevisiae: a review and perspective

Production of fuels and chemicals from xylose by engineered Saccharomyces cerevisiae: a review and perspective

Utilization of biodiesel-derived glycerol or xylose for increased growth and lipid production by indigenous microalgae

A novel pathway to produce butanol and isobutanol in Saccharomyces cerevisiae

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