Ischemic Postconditioning Does Not Attenuateï¿½Ischemia-Reperfusion Injury of Rabbit Small Intestine

Bretz, Brian; Blaze, C. A.; Parry, N.; Kudej, Raymond K.

doi:10.1111/j.1532-950x.2009.00619.x

Cited by 19 publications

(19 citation statements)

References 55 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This technique has already been proven as effective in intestinal I/R protection [9] , nevertheless, Nakamura et al [18] recently demonstrated that five cycles with a duration of thirty seconds each for IPC were not able to prevent reperfusion injury in rats. Failure to protect tissue through IPC using short cycles had already been published previously by Bretz et al [19] , who performed jejunal I/R in rabbits. The authors used IPC for four cycles of 30 seconds each, as used here, without showing advantages over the control group.…”

Section: Discussionmentioning

confidence: 99%

Ischemic Postconditioning Assessment in the Liver of Rats Undergoing Mesenteric Ischemia and Reperfusion

Santos¹,

Aydos²,

Neto³

et al. 2016

Brazilian Journal of Cardiovascular Surgery

View full text Add to dashboard Cite

IntroductionIschemic postconditioning is a method that shows evidence of efficacy in minimizing reperfusion injury; however, its effectiveness in preventing injuries in distant organs is still unknown, especially in those who have undergone mesenteric ischemia and reperfusion.ObjectiveTo evaluate the effect of ischemic postconditioning in preventing reperfusion injury in the liver of rats submitted to mesenteric ischemia and reperfusion, comparing two different methods of ischemic postconditioning.Methods30 Wistar male rats were used, distributed into three groups: Group A: Ten rats submitted to intestinal ischemia for 30 minutes followed by reperfusion for 60 minutes; Group B: Ten rats subjected to ischemia and reperfusion; after ischemia, two cycles of reperfusion (two minutes each) interleaved with two cycles of ischemia (two minutes each); and Group C: Ten rats subjected to ischemia and reperfusion; after ischemia, four cycles of reperfusion (30 seconds each) interspersed with four cycles of ischemia (30 seconds each). After the experiment, the left lobe of the liver was resected for subsequent histological analysis, using the following classification: grade 1 - centrilobular congestion; grade 2 - centrilobular congestion with some degeneration of hepatocytes in one or two central veins; and grade 3 - multifocal centrilobular congestion and degeneration of portal hepatocytes.ResultsThe mean degree of liver damage found was 1.8 in group A, 1.7 in group B and 1.3 in group C. There was no statistically significant difference between the groups.ConclusionIschemic postconditioning was unable to minimize reperfusion injury in rats undergoing mesenteric ischemia and reperfusion.

show abstract

Section: Discussionmentioning

confidence: 99%

Ischemic Postconditioning Assessment in the Liver of Rats Undergoing Mesenteric Ischemia and Reperfusion

Santos¹,

Aydos²,

Neto³

et al. 2016

Brazilian Journal of Cardiovascular Surgery

View full text Add to dashboard Cite

show abstract

“…Introduced in the medicine universe by Murry et al 3 , discovering the ischemic preconditioning protection in hearts of dogs submitted to temporary occlusion and reported to occur also in humans by Yellon et al 4 , the occlusion by selective clamping of the cranial mesenteric artery in rats being the routine procedure until now in most reported mesenteric circulation research. Mesenteric Ischemia-reperfusion study in rabbits is rare and as reported by Bretz et al 23 with different and bad protective results than observed with the mesenteric ischemic pre and postconditioning in rats.…”

Section: Discussionmentioning

confidence: 95%

“…Nowadays the ischemia-reperfusion stress is confirmed to affect with different intensity different animal species but with still little reports in the literature regarding rabbit intestinal mucosa response 5 . The objective of this study is to evaluate the ischemic preconditioning effect in the protection of the histopathology lesions of the intestinal mucosa of rabbits submitted to mesenteric ischemia considering the particular anatomy standard 6 of the collateral mesenteric circulation in these animals.…”

Section: Introductionmentioning

confidence: 99%

Ischemia-reperfusion histopathology alterations of the rabbit intestinal wall with and without ischemic preconditioning

Gomes¹,

Filho

Salles

et al. 2011

Acta Cir. Bras.

View full text Add to dashboard Cite

. Provided institutional support for the research development. ABSTRACT Purpose:To evaluate the histopathology alterations of the intestinal mucosa of rabbits submitted to mesenteric artery ischemia and reperfusion with and without ischemic preconditioning. Methods: Two groups of ten male New Zealand white rabbits body (weight 2.2-3.0, average 2.5 kg). For mesenteric ischemia induction in all animals the small bowel and mesentery were cut 30cm and 60cm far from the gastroduodenal pyloric transition before the proximal mesenteric artery occlusion. In the Group 1 animals, the proximal mesenteric artery was occluded for 45 min with an atraumatic vascular clamp, followed by reperfusion for 30 min. In the Group 2 the 45 min ischemic phase was preceded by three cycles of ischemia (2 minutes each) alternated with three cycles of reperfusion (2 minutes each). For istopathology study small bowel biopsies were obtained before ischemia (control), after 45 min of mesenteric ischemia and at 30 min. of mesenteric artery reperfusion. Results: In the Group I animals, the followings histopathology grade results were observed: t1, mean 2,8; t2, mean 3,3. Using the Kruskal-Wallis non-parameter test, differences between t0 and t1 and t0 and t2 were significants (p<0.05), but not significant between t1 and t2 (p>0.05). In the Group 2 animals histopathology grade results were: t1 mean 2,6 and t2, mean 2,1. Differences between t0 and t1, t0 and t2 were significant (p<0.05). It was not observed differences (p>0.05) between results of t1 in both groups but histopathology injury observed in Group 1 t2 biopsies were higher (p<0.05) than observed in the same period (t2) of Group 2 animals. Conclusion: Microscopic examination of the biopsies revealed significant evidence of preconditioning protection against small bowel wall ischemia-reperfusion injury. Key words: Mesenteric Vascular Occlusion. Ischemic Preconditioning. Animal Experimentation. Rabbits. RESUMOObjetivo: Avaliar as alterações histopatológicas da mucosa intestinal de coelhos submetidos a isquemia-reperfusão com e sem precondicionamento isquêmicol. Métodos: Foram estudados dois grupos de dez coelhos Nova Zelândia machos com pesos variáveis entre 2,2 e 3,0 kg (média de 2,5 kg) de peso corpóreo. Para indução da isquemia, em todos os animais, o intestino delgado e o mesentério foram seccionados 30 cm e 60 cm após a transição pilórica gastroduodenal, antes da oclusão da artéria mesentérica cranial. Nos animais do Grupo 1, a artéria mesentérica proximal foi ocluida por pinçamento atraumático durante 45 min., seguido de reperfusão por 30 min. No Grupo 2, foi realizado precondicionamento por três ciclos de 2 min. de oclusão mesentérica intercalados com três ciclos de 2 min. de reperfusão, seguido de oclusão mantida por 45 min e reperfusão de 30min. como no Grupo I. Para estudo histopatológico, foram obtidas biópsias da parede intestinal antes da isquemia (t 0 -controle), após 45 min. de isquemia (t 1 ) e após 30 min. de reperfusão (t 2) . Resultados: No Grupo I foram observados os segu...

show abstract

“…A 2.5-mm angioplasty balloon (Cordis) was then positioned in the mid-distal portion of the left anterior descending (LAD) artery, below the second diagonal branch. Acute myocardial infarction was induced by balloon inflation for 45 min in the LAD artery [8,9]. After the balloon was inflated at 6-8 atm, another coronary angiography was performed to confirm the complete occlusion of the LAD (Fig.…”

Section: Experimental Modelmentioning

confidence: 99%

Ischemic postconditioning through percutaneous transluminal coronary angioplasty in pigs

Ma¹,

Yin²,

Guo³

et al. 2012

Coronary Artery Disease

View full text Add to dashboard Cite

show abstract

Ischemic Postconditioning Does Not Attenuateï¿½Ischemia-Reperfusion Injury of Rabbit Small Intestine

Abstract: Further experimental studies will need to be performed before clinical application of postconditioning for intestinal ischemia.

Cited by 19 publications

References 55 publications

Ischemic Postconditioning Assessment in the Liver of Rats Undergoing Mesenteric Ischemia and Reperfusion

Ischemic Postconditioning Assessment in the Liver of Rats Undergoing Mesenteric Ischemia and Reperfusion

Ischemia-reperfusion histopathology alterations of the rabbit intestinal wall with and without ischemic preconditioning

Ischemic postconditioning through percutaneous transluminal coronary angioplasty in pigs

Contact Info

Product

Resources

About