Is saliva a reliable biofluid for the detection of COVID-19?

Torul, Damla; Ömezli, Mehmet Melih

doi:10.17219/dmp/132515

Cited by 16 publications

(19 citation statements)

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“…Disinfection should be a routine procedure in dental offices and dental laboratories, allowing prevention of the spread of pathogens and limitation of biological risks of both self-contamination and cross-contamination at each stage of the fabrication of oral appliances, as impression, casts, and final works are transferred between dental office and laboratory several times [ 8 ]. In particular, the current situation of the SARS-CoV2 pandemic requires special care for safety concerns, highlighting the great importance of the effective disinfection of dental impressions [ 9 , 10 ]. Among the various available methods, autoclave sterilization would provide very effective microbial elimination, but, on the other hand, high temperature can cause damage to the material or loss of its properties, including tear and tensile strength, as well as dimensional changes [ 11 ].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Antimicrobial Efficacy of UVC Radiation, Gaseous Ozone, and Liquid Chemicals Used for Disinfection of Silicone Dental Impression Materials

et al. 2022

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Effective disinfection of dental impressions is an indispensable requirement for the safety of dental personnel and patients. The ideal method should be not only effective but also convenient, cheap, and environmentally friendly. This study aimed to reliably evaluate the efficacy of ultraviolet C (UVC) radiation, gaseous ozone, and commercial liquid chemicals used for silicone dental impressions disinfection. These methods were applied to two types of elastomeric impression materials: condensation silicones and addition silicones of various consistency (putty, medium, and light). The antimicrobial effectiveness against Pseudomonas aeruginosa, Staphylococcus aureus, and Candida albicans was evaluated in vitro by counting colony-forming units (CFU) on the surface of samples. The one-way ANOVA with a Tukey HSD test or the Kruskal–Wallis with a Dunn’s test was performed. The results obtained revealed the efficacy of the proposed methods for disinfection of both C-silicones and A-silicones in most of the studied groups. Only one material (Panasil initial contact Light) was not effectively disinfected after UVC irradiation or ozone application. In conclusion, the potential of each disinfection method should be evaluated separately for each material. Moreover, in further research, the possible influence of the proposed methods on the physical properties of the impression materials should be thoroughly investigated.

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Section: Introductionmentioning

confidence: 99%

Evaluation of Antimicrobial Efficacy of UVC Radiation, Gaseous Ozone, and Liquid Chemicals Used for Disinfection of Silicone Dental Impression Materials

et al. 2022

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“…Recently, the saliva has emerged as a convenient and cost-effective biofluid for COVID-19 diagnostics and may eventually replace a nasopharyngeal swab. 22,23 The collection of the saliva is non-invasive, inexpensive and uses a simple technique without the need for special equipment. Since monkeypox infection often manifests in the oral cavity, the saliva in patients may harbor the virions of monkeypox and, therefore, could potentially be used as the biosample to detect the virus.…”

Section: Diagnosismentioning

confidence: 99%

Oral manifestations of monkeypox: Brief review

Iamaroon¹

2022

Dent. Med. Probl.

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The current outbreak of monkeypox infection has caught the attention of people worldwide. Updated data showed a dramatic increase in the number of patients in many non-endemic countries. An emergence of monkeypox together with coronavirus disease 2019 (COVID-19) caused a tremendous burden on the healthcare system, globally.The aim of this review was to briefly describe the current situation, transmission, clinical features, diagnosis, and prevention of the disease. Oral manifestations of monkeypox as well as those of similar viral diseases were elaborately delineated.The outbreak of monkeypox in non-endemic regions has expanded to at least 47 countries with more than 4,100 new infections. The clinical features in non-endemic regions are atypical and different from those in central and western Africa. Milder symptoms with no death cases have been observed. The oral mucosa is often involved and oral lesions may initially be manifested before the rash spreads to the face and other parts of the body. The diagnosis of monkeypox is mainly based on clinical presentations and laboratory investigations. Prevention by avoiding close contacts with patients and sick animals and providing vaccination to those who have a primary contact with patients is essential.Oral manifestations may occur prior to skin eruptions, suggesting that dentists and dental personnel should be well aware of the nature of the disease. Prevention and public awareness of the disease are crucial for mitigating further human-to-human transmission.

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“…19 As an alternative to nasopharyngeal swabs, saliva has been reported to be a safe and reliable tool for the diagnosis of COVID-19, offering greater safety, and logistic and economic benefits. 20 Due to the lack of clinical data, the aim of this study was to clinically evaluate the effect of the HClO and PVP-I solutions on the viral load of SARS-CoV-2 in COVID-19 patients. The null hypothesis was that there would be no difference in reducing the SARS-CoV-2 viral load between the HClO or PVP-I solutions.…”

Section: Introductionmentioning

confidence: 99%

Effect of oral antiseptics on the viral load of SARS-CoV-2: A randomized controlled trial

Gül¹,

Dilsiz²,

Sağlık³

et al. 2022

Dent. Med. Probl.

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Background. In the oral cavity, which plays an important role in the transmission of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), it is possible to reduce the viral load of SARS-CoV-2 with antiseptics, thereby minimizing the transmission of the virus during dental procedures.Objectives. The aim of this study was to clinically evaluate the effect of the hypochlorous acid (HClO) and povidone-iodine (PVP-I) solutions on the oral viral load of SARS-CoV-2.Material and methods. This randomized controlled trial was conducted on 75 patients hospitalized in the COVID-19 ward of a local hospital. All the patients included in the study were within the first 24 h of hospitalization and the first 5 days of coronavirus disease 2019 (COVID-19) symptoms. The viral load of mouthwash samples was measured with the cycle threshold (Ct) value of SARS-CoV-2 through a realtime reverse transcription polymerase chain reaction (RT-PCR). The patients were divided into 3 groups. The effect on the patient's SARS-CoV-2 viral load was investigated after gargling the mouths and throats for 30 s with HClO, PVP-I and isotonic saline. First, a sample was taken after gargling with isotonic saline, then another sample was taken after gargling for 30 s with a particular antiseptic to determine the viral load of SARS-CoV-2. Results.Comparing the before and after mouthwash samples from all 3 groups, there were no statistically significant differences in the Ct values before and after gargling (p > 0.05). However, there were statistically significant differences in the number of negative samples after the use of HClO and PVP-I, which were positive before gargling (p < 0.05). Conclusions.In the light of the data obtained in this study, there is insufficient evidence that gargling with HClO or PVP-I reduces viral load. Taken together, these findings imply no role for antiseptics in the transmission of SARS-CoV-2 by the aerosol generated during dental procedures, or more generally, SARS-CoV-2 infection control.

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Is saliva a reliable biofluid for the detection of COVID-19?

Cited by 16 publications

References 0 publications

Evaluation of Antimicrobial Efficacy of UVC Radiation, Gaseous Ozone, and Liquid Chemicals Used for Disinfection of Silicone Dental Impression Materials

Evaluation of Antimicrobial Efficacy of UVC Radiation, Gaseous Ozone, and Liquid Chemicals Used for Disinfection of Silicone Dental Impression Materials

Oral manifestations of monkeypox: Brief review

Effect of oral antiseptics on the viral load of SARS-CoV-2: A randomized controlled trial

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