Is enrichment broth necessary for stool cultures?

Lue, Yvonne A.

doi:10.1016/0196-4399(86)90097-8

Cited by 10 publications

(5 citation statements)

References 3 publications

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“…However, many laboratories have discontinued this practice, as the yield does not justify the cost (274). Historic data from individual laboratories should be used to help determine if subcultures from enrichment broth should be used or discontinued.…”

Section: Isolation Of E Coli O157 (Colorless On This Medium)mentioning

confidence: 99%

Practical Guidance for Clinical Microbiology Laboratories: Diagnosis of Bacterial Gastroenteritis

2015

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SUMMARY Bacterial gastroenteritis is a disease that is pervasive in both the developing and developed worlds. While for the most part bacterial gastroenteritis is self-limiting, identification of an etiological agent by bacterial stool culture is required for the management of patients with severe or prolonged diarrhea, symptoms consistent with invasive disease, or a history that may predict a complicated course of disease. Importantly, characterization of bacterial enteropathogens from stool cultures in clinical laboratories is one of the primary means by which public health officials identify and track outbreaks of bacterial gastroenteritis. This article provides guidance for clinical microbiology laboratories that perform stool cultures. The general characteristics, epidemiology, and clinical manifestations of key bacterial enteropathogens are summarized. Information regarding optimal specimen collection, transport, and processing and current diagnostic tests and testing algorithms is provided. This article is an update of Cumitech 12A (P. H. Gilligan, J. M. Janda, M. A. Karmali, and J. M. Miller, Cumitech 12A, Laboratory diagnosis of bacterial diarrhea, 1992).

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Section: Isolation Of E Coli O157 (Colorless On This Medium)mentioning

confidence: 99%

Practical Guidance for Clinical Microbiology Laboratories: Diagnosis of Bacterial Gastroenteritis

2015

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“…Historically, broth enrichment has been used to increase detection of bacterial pathogens especially in times of extended sample transport to the laboratory. Although an overabundance of evidence points to the utility of enrichment, the cost-effectiveness and value of enrichment broths has been questioned [19]. The increased financial burden to PHL having to recover isolates from CIDT positive stool specimens eliminates the potential for pathogen-specific gold-standard enrichment to be used routinely.…”

Section: Introductionmentioning

confidence: 99%

Development of an Enteric Bacterial Enrichment Broth and Its Performance for Isolation of Clinically Significant Bacterial Pathogens from Stool

Horseman¹,

Lustik²,

Fong³

2021

OJMM

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Background: Early detection and accurate identification of foodborne pathogen outbreaks is an important public health function. Increased clinical adoption of multiplex PCR assays or culture independent diagnostic tests (CIDT) correlates to more stool specimens sent to public health laboratories (PHL) for characterization. Isolation and confirmation of enteric bacterial pathogens can prove difficult to consistently recover. The purpose of this study was to evaluate the performance of a broad-use laboratory developed enrichment broth for isolation of Campylobacter, Salmonella, Shigella, and Yersinia strains from stool specimens. Methods: The study compared differences in positivity rates among media and enrichment combinations at specific time points. Comparison of direct inoculation (DI), enrichment using a lab-developed Enteric Bacterial Enrichment (EBE) broth and gold-standard isolation methods were conducted to test current utility of this established practice with stool specimens heat injured and non-injured. Results: A total of 234 spiked stool samples, 175 non-injured and 59 heat injured, were tested with varying bacterial concentrations. For non-injured stools, direct inoculation performed better for Campylobacter and Yersinia than enrichment. Conversely, Salmonella and Shigella recovery and limit of detection increased with enrichment. Campylobacter had the highest percent recovery while Shigella being the lowest from direct plating at 6-hour and 24-hour enrichment periods. Among broths, EBE performed the best for Yersinia and similar to Selenite broth for Salmonella and Shigella. Generally, heat injured stool had a significantly lower percent of recovery than non-heat injured with a higher limit of detection across organisms. Conclusion: Our data suggest there is an only utility for targeted enrichment of CIDT positive Salmonella stool specimens.

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“…In most laboratories where stool samples are cultured for S. enterica, both solid medium and an enteric enrichment broth such as Selenite are inoculated directly with the specimen. It has been suggested that enrichment broth be used only during outbreaks, for screening for asymptomatic carriage, and in other clinically warranted situations (3,4). We have conducted a study on stool specimens submitted to the Microbiology Laboratory in University College Hospital, Galway, Ireland, over a one-year period (July 1996 to June 1997) to determine if inoculation of both enrichment broth and a primary xylose lysine deoxycholate (XLD) agar plate (Oxoid) is of value.…”

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confidence: 99%

Cost-Effective Methods for Isolation of Salmonella enterica in the Clinical Laboratory

et al. 1999

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Is enrichment broth necessary for stool cultures?

Cited by 10 publications

References 3 publications

Practical Guidance for Clinical Microbiology Laboratories: Diagnosis of Bacterial Gastroenteritis

Practical Guidance for Clinical Microbiology Laboratories: Diagnosis of Bacterial Gastroenteritis

Development of an Enteric Bacterial Enrichment Broth and Its Performance for Isolation of Clinically Significant Bacterial Pathogens from Stool

Cost-Effective Methods for Isolation of Salmonella enterica in the Clinical Laboratory

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