Is duality between proline metabolic mutation (p5cs 1-4) and durum wheat dehydrin transgenic contexts a “pacemaker” for salt tolerance process in Arabidopsis thaliana?

Causal Enzymology and Physiological Aspects May Be Accountable to Membrane Integrity in Response to Salt Stress in Arabidopsis thaliana Lines

“…Furthermore, DHN-5 overexpression in Arabidopsis thaliana was realized by our team [23]. Indeed, we proved that DHN-5 confers salinity tolerance to the dehydrin transgenic Arabidopsis lines (DHE 2, DH4, and K 1 -K 2 ), through the modulation of some of metabolic pathways such as ROS scavenging system, proline metabolism one, and also some of phytozymes like proteases (cysteine and aspartyl proteases) [3, 5].…”

“…Indeed, the level of MDA in transgenic lines was significantly lower (5.4, 5.5, and 5.4 mmol.g −1 (FW) in DH2, DH4, and K 1 -K 2 , respectively) than in Wt, YS, and P5CS 1-4 (11.35 and 9.7 mmol.g −1 , 0 mmol.g −1 (FW), respectively). In addition to this fact, it is crucial to note that the MDA level of the P5CS 1-4 mutant line under salt stress treatment is considered to be zero because of the inability of the described strain to grow under salt stress conditions used in these studies [3].…”

“…The best characterized biochemical response of plant cells to osmotic stress is the accumulation of organic osmolytes like proline [2, 3, 5, 6]. The accumulation of this atypical amino acid in leaves was first observed [7] and since then its cardinal role as an osmoprotectant effector under various stress conditions, especially under salt stress, has been shown [8].…”

“…On the other hand, one of the known plant responses to salt stress is ROS production [5, 12]. Plant cells need to regulate ROS production as excess ROS is potentially harmful to nucleic acids, proteins, and lipids and may therefore lead to cell injury and death [1, 3, 14, 15]. ROS produced through NADPH oxidase activity was shown to be mediated by phospholipid signaling [12, 15].…”

Proline, A Peculiar Amino Acid with Astucious Functions in Development and Salt Tolerance Process in Plants

“…Proline is an amino acid with pivotal role in plant responses to environmental constraints [1][2][3][4]. Their amendment positively affected plant water relations, due mostly to enhancement in osmoregulation, as certain genotypes with greater mean leaf proline concentration and relative water content appeared to produce greater above-ground dry mass, when exposed to the external proline [4][5][6][7]. Indeed, exogenous proline resulted in significant increases in various phytometabolites such as polyphenol, carotenoids, chlorophyll, proline, carbohydrates, essential oil amounts and relative water content [3,7,8].…”