Iron metabolism in bifidobacteria

Bezkorovainy, Anatoly; Kot, Eva; Miller-Catchpole, Robin; Haloftis, George; Furmanov, Sergey Mikhailovich

doi:10.1016/s0958-6946(96)00003-9

Cited by 25 publications

(14 citation statements)

References 35 publications

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“…In the present study, the incorporation of probiotics into the gels was performed under aseptic conditions. Therefore, the only bacteria observed in scanning electron microscopy are B. longum and B. adolescentis, which come from the reference ATCC cultures used; besides, typical morphology, sizes, and shapes are in agreement with those reported in the literature [26,27].…”

Section: Microscopysupporting

confidence: 85%

Maize Processing Waste Water Upcycling in Mexico: Recovery of Arabinoxylans for Probiotic Encapsulation

et al. 2016

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Maize is a major source of food in Mexico. In order to improve its nutritional value, maize kernel is exposed to an alkali treatment that generates large volumes of waste water containing gelling arabinoxylan. The purpose of the present study was to evaluate the capability of maize waste water arabinoxylans (MWAX) to encapsulate probiotics. The rheological, structural, and microstructural characteristics of this bio-based material were also investigated. MWAX gels at 10% (w/v) were able to encapsulate Bifidobacterium as probiotic model. The MWAX gel containing 1 × 10 7 CFU/mL of probiotics presented a storage (G ) and loss (G") moduli of 50 and 11 Pa, respectively. The average mesh size of the MWAX gel was around 11 times smaller than the Bifidobacterium cell magnitude. MWAX gels with or without probiotics were studied using scanning electron microscopy. The interior of the Bifidobacterium loaded gels was composed of a pore-like network of MWAX through which probiotics were distributed. The probiotic encapsulating MWAX gels appeared to be less porous than the empty gels. MWAX capability to encapsulate Bifidobacterium may be important in designing probiotic encapsulating biodegradable gels and could represent an opportunity in sustainable food waste management and utilization through upcycling to value-added products.

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Section: Microscopysupporting

confidence: 85%

Maize Processing Waste Water Upcycling in Mexico: Recovery of Arabinoxylans for Probiotic Encapsulation

et al. 2016

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“…Lactobacilli do not produce siderophores, and their growth is similar in media with and without iron 38 . Bifidobacterium breve , an important bifidobacteria species in breastfed infants, can sequester luminal iron using a divalent metal permease,39 40 but the majority of bifidobacteria do not produce siderophores or other active iron carriers. Abundant bifidobacteria, lactobacilli and other beneficial bacteria in the colon provide an important ‘barrier effect’ against colonisation and invasion by pathogens 17 41 42.…”

Section: Discussionmentioning

confidence: 99%

Iron fortification adversely affects the gut microbiome, increases pathogen abundance and induces intestinal inflammation in Kenyan infants

Jaeggi

Kortman

Moretti

et al. 2014

Gut

516

510

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“…In bifidobacteria, oligosaccharides and polysaccharides are depolymerized down to their monomeric constituents, which are incorporated into the fructose-6-phosphate shunt, giving lactic and acetic acids as the major products and ethanol and formic acid as the minor ones (2,35,42). Hexoses are broken down throughout the shunt so that the carbon flux is equally divided into two-carbon and three-carbon molecules.…”

Section: Discussionmentioning

confidence: 99%

“…In order to give an explanation of the carbohydrate preferences of B. adolescentis MB 239, carbon fluxes and ATP yields were calculated on the basis of a fructose-6-phosphate shunt (Fig. 1), the peculiar pattern of hexose catabolism in the genus Bifidobacterium (2,35,42).…”

mentioning

confidence: 99%

Kinetics and Metabolism of Bifidobacterium adolescentis MB 239 Growing on Glucose, Galactose, Lactose, and Galactooligosaccharides

Amaretti

Bernardi

Tamburini

et al. 2007

Appl Environ Microbiol

100

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The kinetics and the metabolism of Bifidobacterium adolescentis MB 239 growing on galactooligosaccharides (GOS), lactose, galactose, and glucose were investigated. An unstructured unsegregated model for growth in batch cultures was developed, and kinetic parameters were calculated with a recursive algorithm. The growth rate and cellular yield were highest on galactose, followed by lactose and GOS, and were lowest on glucose. Lactate, acetate, and ethanol yields allowed the calculation of carbon fluxes toward fermentation products. Distributions between two-and three-carbon products were similar on all the carbohydrates (55 and 45%, respectively), but ethanol yields were different on glucose, GOS, lactose, and galactose, in decreasing order of production. Based on the stoichiometry of the fructose-6-phosphate shunt and on the carbon distribution among the products, the ATP yield was calculated. The highest yield was obtained on galactose, while the yields were 5, 8, and 25% lower on lactose, GOS, and glucose, respectively. Therefore, a correspondence among ethanol production, low ATP yields, and low biomass production was established, demonstrating that carbohydrate preferences may result from different distributions of carbon fluxes through the fermentative pathway. During the fermentation of a GOS mixture, substrate selectivity based on the degree of polymerization was exhibited, since lactose and the trisaccharide were the first to be consumed, while a delay was observed until longer oligosaccharides were utilized. Throughout the growth on both lactose and GOS, galactose accumulated in the cultural broth, suggesting that ␤(1-4) galactosides can be hydrolyzed before they are taken up.Many nondigestible oligosaccharides, such as fructooligosaccharides, isomaltooligosaccharides, galactooligosaccharides (GOS), and xylooligosaccharides, have been reported to beneficially affect human health. They are defined as prebiotics and are increasingly being used as functional food ingredients (18). ␤-GOS are manufactured from highly concentrated lactose solutions by the action of ␤-galactosidases (␤-Gals) which have transgalactosylation activity. In standardized large-scale production, trisaccharides to hexasaccharides are the main products; hence, commercial GOS occur as mixtures of various degrees of polymerization (DP) and glycosidic linkages and contain large amounts of glucose and unreacted lactose. The linkage between galactose moieties (mainly ␤1-4, ␤1-6, and ␤1-3), the efficiency of transgalactosylation, and the components in the final product depend upon the enzymes and the reaction conditions (3,7,25,30,34,39,41).As demonstrated in several in vitro and in vivo studies (5, 40), GOS resist hydrolysis by human digestive enzymes and are not absorbed on transit through the small intestine; therefore, they are available for fermentation by the colon-resident microflora. The utilization of GOS by the major intestinal bacteria has been investigated (25,34,46). It was shown that ␤1-4-linked oligosaccharides were selectivel...

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Iron metabolism in bifidobacteria

Cited by 25 publications

References 35 publications

Maize Processing Waste Water Upcycling in Mexico: Recovery of Arabinoxylans for Probiotic Encapsulation

Maize Processing Waste Water Upcycling in Mexico: Recovery of Arabinoxylans for Probiotic Encapsulation

Iron fortification adversely affects the gut microbiome, increases pathogen abundance and induces intestinal inflammation in Kenyan infants

Kinetics and Metabolism of Bifidobacterium adolescentis MB 239 Growing on Glucose, Galactose, Lactose, and Galactooligosaccharides

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