Iron-core/carbon-shell nanoparticles with intrinsic peroxidase activity: new platform for mimetic glucose detection

Abstract

Artificial enzymatic mimics based on nanoparticles have become a powerful tool for the improvement of analytical performance in the detection of important bioactive compounds. For the first time the intrinsic peroxidase-like activity of Fe-core/carbon shell nanoparticles (Fe@C NPs) was studied. The catalytic process was described by a typical Michaelis-Menten curve for enzyme kinetics, and the results were comparable with those previously published. The high catalytic performance of the Fe@C NPs allows the development of a new simple procedure for glucose determination with a low detection limit of 0.21 mM. To our knowledge, this is the first study showing the ability to generate active oxygen species on the Fe@C surfaces. We suppose that our investigation will open up a new direction in medicinal applications using these promising materials.

Carbon Nanozymes: Enzymatic Properties, Catalytic Mechanism, and Applications

“…Since H 2 O 2 is ap roduct of glucose oxidized by O 2 in the presence of glucose oxidase (GOx), the CNM-catalyzed reaction can be readily adapted to detect glucose (Table S2). [35,[39][40][41]44,47,50,57,61,64,67,68,75,79,80,82,91,95,96,99,111,114,120,143,149,150,154,157,170] According to the above working principle, afast, simple,sensitive,and selective colorimetric method for glucose detection with alimit of detection as low as 1 mm has been presented ( Figure 4). [35] Theprotocol is reliable enough and has been used for analysis of glucose in actual samples, such as diluted blood and commercial fruit juices.…”

“…Besides their intrinsic enzymatic activity, CNMs and their derivatives can be also utilized as modulators to further improve the catalytic activity and stability of artificial peroxidase. Many classic artificial peroxidases, for instance, metal oxides (for example, Fe 3 O 4 , FeO x H, Fe 2 O 3 , Co 3 O 4 , ZnO, CuO, Cu 2 O, CeO 2 , CoFe 2 O 4 , and WO 3 ), metal hydroxide, metal chalcogenides (for example, CoSe 2 , CuS, and MoS 2 ), metal nanoparticles (for example, Pt, Pd, Au, Ag, and Fe), metal alloys (for example, PtPd, PdNi, AuPd, AuAg, CuAg, and FeCo), metal ions (for example, Cu 2+[152] ), complexes (for example, hemin, chiral metallo‐supramolecular complex, Fe‐phenanthroline, and prussian blue…”

Peroxidase activity of new mixed‐valence cobalt complexes with ligands derived from pyridoxal

“…OPD is a water‐soluble compound used as a substrate to evaluate the mimetic peroxidase activity of cobalt complexes. In contact with hydrogen peroxide and a catalyst, this initial substrate produces an orange/yellow‐colored product (formation of 2,3‐diaminophenazine (DAP) is spectrophotometrically monitored at 450 nm) . To obtain the buffer solution, 0.6 g of Na 2 HPO 4 (anhydrous; 8.45 mM), 0.11 g of NaH 2 PO 4 ·H 2 O (1.59 mM), and 4.3 g of NaCl (0.15 M) were added to 500 ml of distilled water.…”

Kohlenstoff‐Nanozyme: Enzymatische Eigenschaften, Katalysemechanismen und Anwendungen

“…Viele klassische künstliche Peroxidasen, z. B. Metalloxide (Fe 3 O 4 , FeO x H, Fe 2 O 3 , Co 3 O 4 , ZnO, CuO, Cu 2 O, CeO 2 , CoFe 2 O 4 , WO 3 ), Metallhydroxide, Metallchalkogenide (CoSe 2 , CuS, MoS 2 ), Metallnanopartikel (Pt, Pd, Au, Ag, Fe), Legierungen (PtPd, PdNi, AuPd, AuAg, CuAg, FeCo), Metallionen (Cu 2+[152] ), Komplexe (Hämin, chirale metallosupramolekulare Komplexe, Fe‐Phenanthrolin, Preußischblau), wurden verwendet, um Hybride mit CNMs herzustellen. Wegen ihrer großen spezifischen Oberflächen, ihren ausgezeichneten physikalischen Eigenschaften und ihrer vielseitigen Oberflächenchemie spielten CNMs sehr wichtige Rollen in der Regulierung der enzymatischen Aktivität von...…”

“…Da H 2 O 2 ein Produkt der Glukose‐Oxidation durch O 2 in der Gegenwart von Glukose‐Oxidase (GOx) ist, kann die CNM‐katalysierte Reaktion zum Nachweis von Glukose verwendet werden (Table S2 in den Hintergrundinformationen) . Basierend auf diesem Prinzip wurde eine schnelle, einfache, empfindliche und selektive kolorimetrische Methode für den Glukose‐Nachweis mit einer Nachweisgrenze von 1 μ m vorgestellt (Abbildung ) .…”