Artificial enzymatic mimics based on nanoparticles have become a powerful tool for the improvement of analytical performance in the detection of important bioactive compounds. For the first time the intrinsic peroxidase-like activity of Fe-core/carbon shell nanoparticles (Fe@C NPs) was studied. The catalytic process was described by a typical Michaelis-Menten curve for enzyme kinetics, and the results were comparable with those previously published. The high catalytic performance of the Fe@C NPs allows the development of a new simple procedure for glucose determination with a low detection limit of 0.21 mM. To our knowledge, this is the first study showing the ability to generate active oxygen species on the Fe@C surfaces. We suppose that our investigation will open up a new direction in medicinal applications using these promising materials.
The Fe-core/carbon-shell nanopowders are excellent platform for covalent grafting of biomolecules. The large-scale synthesis of Fe-core/carbon-shell nanoparticles via electropulse erosion of metal precursors in hydrocarbons was developed. The green fluorescent protein was covalently attached to the powder surface via diazonium functionalization and further carbodiimide activation.
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