Iridoid glycosides extracted from Zhizi (Fructus Gardeniae) decrease collagen-induced platelet aggregation and reduce carotid artery thrombosis in an in vivo rat model

Wang, Peng; Wang, Qunxing; Luo, Chao; Tan, Chao; Yuan, Xiaofeng

doi:10.1016/s0254-6272(13)60160-0

Cited by 22 publications

(11 citation statements)

References 16 publications

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“…Pretreatment with IGs for 3 days with dose of 100 mg/kg gave 13% platelet aggregation ratio comparable to aspirin (18%) at 40 mg/kg. [ 28 ]. Another compound of Gardenia jasminoides Ellis called genipin (GE) with different concentrations has shown significant inhibition on the collagen-induced platelet aggregation in vitro , but only a little effect on that induced by ADP and AA [ 29 ].…”

Section: Resultsmentioning

confidence: 99%

Medicinal Herbals with Antiplatelet Properties Benefit in Coronary Atherothrombotic Diseases

2016

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Coronary atherothrombotic diseases such as coronary artery disease, peripheral vascular disease, cerebrovascular disease, and heart failure are the serious concerns of the thrombus formed in blood vessels. Anticoagulant and antiplatelet drugs are the cornerstones of the management of these diseases. To prevent the recurrence of these diseases, double antiplatelet drugs such as aspirin and clopidogrel has been the standard management in most hospitals. However, aspirin resistance and clopidogrel inefficient effects due to noncompliance with double drugs regimen can cause a sinister effect on patients. Medicinal plants serve as a greater resource for new medication and their potential currently became a topic of interest to the researchers all over the world. Traditionally, certain herbs have been used as a treatment for heart diseases but have been investigated for their antiplatelet properties. This current review explained few traditional antithrombotic herbals and their antiplatelet properties in vitro and in vivo and this is to be deeply discussed in further research.

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Section: Resultsmentioning

confidence: 99%

Medicinal Herbals with Antiplatelet Properties Benefit in Coronary Atherothrombotic Diseases

2016

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“…Plasma was collected after centrifugation at 1000 × g for 10 min at room temperature. An aliquot of 100 μl plasma was supplemented with series concentrations of P1Cm, ranging from 0.15 to 0.45 mM, 0.15 mM tirofiban or vehicle (PBS, pH 7.4), followed by an incubation of 5 min at 37°C before being sent to an ACL TOP Automatic Coagulation Analyzer (Beckman Coulter, USA; Wang et al, 2013 ). The coagulation parameters, such as activated partial thromboplastin time (APTT), prothrombin time (PT), and thrombin time (TT; Chen et al, 2013 ), were recorded, and each assay was performed in triplicate.…”

Section: Methodsmentioning

confidence: 99%

A Novel Pentapeptide Targeting Integrin β3-Subunit Inhibits Platelet Aggregation and Its Application in Rat for Thrombosis Prevention

Liu

Yan

et al. 2016

Front. Pharmacol.

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Background: Antiplatelet therapy plays a pivotal role in the prevention and treatment of thrombotic diseases. We reported the screening of P1C as a novel integrin-binding peptide from the C-terminal of connective tissue growth factor. Primary study indicated that P1C has potential against platelet aggregation.Objectives: We aimed to find the shortest active unit from the P1C fragments and explore its in vivo and in vitro activities.Methods: A series of truncated P1C fragments was prepared and screened for antiplatelet activity. The most active fragment was evaluated using coagulation assays. Flow cytometry and confocal microscopy were used to determine the interaction between the peptide and the integrin. The in vivo potential was further explored using two types of rat models.Results: From a series of truncated P1C forms, a so-called P1Cm peptide of 5-amino acids, namely, IRTPK was screened out as the shortest active unit with superior activity. Coagulation experiments and an in vivo toxicity assay demonstrated that P1Cm is safe in vivo and inhibits ADP- and TH-induced human platelet aggregation in vitro in a concentration-dependent manner. Furthermore, it has limited effect on the coagulation parameters. Flow cytometry and confocal microscopy experiments consistently indicated that the peptide specifically binds the β3-subunit of integrin on platelets. Further experiments using rat models of artery-vein shunt and carotid arterial thrombosis illustrated that P1Cm can effectively prevent thrombosis formation.Conclusion: P1Cm may be a new, promising antithrombotic alternative to currently available antiplatelet treatments.

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“…110 Anti-thrombotic action of iridoid glycosides (IGs) were assessed that it may potentially contribute to the treatment of cerebral ischemic diseases, including cerebral apoplexy. 111 Anti-hypertensive and anti-thrombotic effects of crocetin led to an increase in bioavailability of NO, possibly mediated by decreased inactivation of NO by reactive oxygen species. 112 …”

Section: Anti-thrombotic Activitymentioning

confidence: 99%

Phytochemistry, Pharmacological Activities and Intellectual Property Landscape of Gardenia jasminoides Ellis: a Review

Phatak¹

2015

Phcog J

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Iridoid glycosides extracted from Zhizi (Fructus Gardeniae) decrease collagen-induced platelet aggregation and reduce carotid artery thrombosis in an in vivo rat model

Abstract: These data, together with the previously reported neuroprotective effects of IGs in rats with cerebral ischemia, suggest that the antithrombotic action of IGs may potentially contribute to the treatment of cerebral ischemic diseases, including cerebral apoplexy.

Cited by 22 publications

References 16 publications

Medicinal Herbals with Antiplatelet Properties Benefit in Coronary Atherothrombotic Diseases

Medicinal Herbals with Antiplatelet Properties Benefit in Coronary Atherothrombotic Diseases

A Novel Pentapeptide Targeting Integrin β3-Subunit Inhibits Platelet Aggregation and Its Application in Rat for Thrombosis Prevention

Phytochemistry, Pharmacological Activities and Intellectual Property Landscape of Gardenia jasminoides Ellis: a Review

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