IR 820 stabilized multifunctional polycaprolactone glycol chitosan composite nanoparticles for cancer therapy

Abstract

Photothermal therapy has gained worldwide attention for its less painful, non invasive/minimally invasive, effective thermal ablation based therapy for cancer. It has been found to be effective in the treatment of drug-resistant cancer. Currently available photothermal systems have several limitations such as a high cost, incompatibility and clearance from the physiological system. To address these issues, we have prepared multifunctional biocompatible and biodegradable cost effective IR 820 dye encapsulated glycol chitosan coated polycaprolactone composite nanoparticles (PCLGC-IR) with improved performance.Polycaprolactone (PCL) has been used as a cheap biodegradable polymer, glycol chitosan (GC) as an immunoadjuvant for immune response generation followed by the cost-effective IR 820 dye for photothermal therapy. IR 820 encapsulation increases the stability of the PCLGC-IR nanoparticles as confirmed by DSC and XRD, while the glycol chitosan enhances the uptake of the composite nanoparticles by MDA-MB-231 breast cancer cells. The PCLGC-IR composite nanoparticles with a size of around 150-200 nm can be irradiated with a dual laser at 750 nm and 808 nm in photothermal therapy (PTT). In addition to PTT, the PCLGC-IR composite nanoparticles can also be used for sustainable controlled drug release as they retain their structural integrity even after laser treatment.Further we analyzed the degraded product using liquid chromatography mass spectroscopy (LCMS) upon photo irradiation. A cytotoxicity study of the PCLGC-IR composite nanoparticles was carried out on NIH3T3 cells. Fluorescence microscope image analysis of MDA-MB-231 cells showed hyperthermic cell death via apoptosis and necrosis due to an enhanced uptake of the PCLGC-IR composite nanoparticles. Our results showed that this nanoformulation can be used for the effective treatment of multiple drug resistant cancer.

IR 820 dye encapsulated in polycaprolactone glycol chitosan: Poloxamer blend nanoparticles for photo immunotherapy for breast cancer

“…Similar shift with IR dye was previously reported by Manchanda et al, wherein they have shown three peaks around 688, 702 and 836 nm upon interaction with PEG amine NPs [28]. In our case, peak shift remains in NIR windows, giving it an additional advantage for the photothermal application [29].…”

“…The ease of conjugation and good stability in blood circulation make it an ideal choice for multimodal imaging and therapy [26][27][28]. In the previous studies, we have focused only on IR 820 dye based photothermal therapy for cancer treatment [29]. This can be further improved by adding immune adjuvant like GC for an immunological response.…”

Chitosan‐based multifunctional nanomedicines and theranostics for targeted therapy of cancer

“…In this regard, Kumar and Srivastava successfully formulated the multifunctional, biocompatible, and biodegradable cost‐effective IR 820 dye encapsulated GC‐coated polycaprolactone nanocomposite (PCLGC–IR). The nanocomposite showed an improved performance of PTT as a theranostic because of retaining their structural integrity even after the laser treatment and showing controlled‐release profile . GC was also shown to increase the cellular uptake of these engineered nanocomposite, resulting in significant perthermic death of MDA‐MB‐231 cells.…”

“…[137][138][139] PTT is a favorable procedure since it is a less painful and noninvasive/minimally showed an improved performance of PTT as a theranostic because of retaining their structural integrity even after the laser treatment and showing controlled-release profile. 141,147 GC was also shown to increase the cellular uptake of these engineered nanocomposite, resulting in significant perthermic death of MDA-MB-231 cells.…”

FITC Conjugated Polycaprolactone-Glycol-Chitosan Nanoparticles Containing The Longwave Emitting Fluorophore IR 820 For In-Vitro Tracking Of Hyperthermia-Induced Cell Death

“…We have previously shown the significance of IR 820 and glycol chitosan (GC) in nanoformulation for effective cancer therapy [18] [19]. IR 820 stabilized PCLGC nanoparticles retain its property as a chemotherapeutic delivery agent even after laser treatment while a GC enhanced the cell death by acting as an immunostimulatory molecule.…”