FITC Conjugated Polycaprolactone-Glycol-Chitosan Nanoparticles Containing The Longwave Emitting Fluorophore IR 820 For In-Vitro Tracking Of Hyperthermia-Induced Cell Death

Abstract

Cancer theranostic agent IR 820 loses its bioimaging ability once therapy is initiated. At the end of therapy, it becomes difficult to track the cancer cells. To address this, FITC conjugated Polycaprolactone glycol chitosan IR 820 nanoparticles (FITC-PCLGC-IR NPs) has been synthesized for in vitro tracking of hyperthemia induced cell death. Two approaches, namely ex situ and in situ have been pursued FITC conjugation to PCLGC-IR NPs. Further comparisons were made to FITC encapsulated PCLGC-IR NPs in terms of biocompatibility, cellular uptake, photothermal mediated cell death and imaging with respect to laser treatment. We have shown that an 808 nm diode laser treatment did not affect the imaging ability of these NPs whereas cancer. Time scanned fluorescence shows the excellent photostability of this formulation for a maximum of 5 min. The detailed studies of these approaches summarize that FITC conjugation to PCLGC-IR nanoparticles is an effective nano-theranostic solution for image-guided photothermal therapy.

Surface Immobilization of Anti-VEGF Peptide on SPIONs for Antiangiogenic and Targeted Delivery of Paclitaxel in Non-Small-Cell Lung Carcinoma

“…FITC-labeled CLA-coated PTX-SPIONs@HRH were prepared using a modified encapsulation method described by Kumar and Srivastava (2018) . Briefly, FITC (1 mg) was dissolved in 1 mL of dichloromethane and methanol (50:50), and the solution was transferred dropwise into a nanoformulation suspension (2.5 mg in 5 mL of distilled water).…”

Short Antiangiogenic MMP-2 Peptide-Decorated Conjugated Linoleic Acid-Coated SPIONs for Targeted Paclitaxel Delivery in an A549 Cell Xenograft Mouse Tumor Model

“…The cellular uptake and internalization of the CTT nanosystem was evaluated using an FITC-labeled nanoformulation. FITC labeling was achieved through a methodology of Kumar and Srivastava (2018), with modifications . Accordingly, 1 mg/mL FITC solution was consituted in methanol: dichloromethane (50:50) and added into a 5 mL suspension of 2.5 mg of CTT nanosystem in distilled water.…”

“…FITC labeling was achieved through a methodology of Kumar and Srivastava (2018), with modifications. 60 Accordingly, 1 mg/mL FITC solution was consituted in methanol: dichloromethane (50:50) and added into a 5 mL suspension of 2.5 mg of CTT nanosystem in distilled water. The sample was allowed to continuously stir, and the solvent was evaporated in the dark for 20 min at room temperature.…”

Chrysin-Loaded Chitosan Nanoparticle-Mediated Neuroprotection in Aβ1–42-Induced Neurodegenerative Conditions in Zebrafish

“…The nanoparticles were conjugated with fluorescein isothiocyanate (FITC) isomer 1 following the protocol described previously. 102 Briefly, 1 mg of FITC isomer was dissolved in 0.1 M PBS. The prepared FITC solution was slowly added to the nanoparticles at a ratio of 1:10 under continuous stirring at low rpm.…”

“…The nanoparticles were conjugated with fluorescein isothiocyanate (FITC) isomer 1 following the protocol described previously . Briefly, 1 mg of FITC isomer was dissolved in 0.1 M PBS.…”