IR 820 dye encapsulated in polycaprolactone glycol chitosan: Poloxamer blend nanoparticles for photo immunotherapy for breast cancer

Abstract

In the present study, we have fabricated biocompatible and biodegradable monodisperse IR 820 encapsulated polycaprolactone (PCL) glycol chitosan (GC): Poloxamer blend nanoparticles (PP-IR NPs) for imaging and effective photo-immunotherapy. IR 820 has been used as an imaging and photothermal agent whereas glycol chitosan (GC) as an immunostimulatory agent. The combination of IR 820, poloxamer, and GC can be used effectively for photoimmunotherapy for cancer, drug-resistant and TNF-α resistant estrogen positive breast cancer. PP-IR NPs are stable in aqueous solution. The uniform size of 100-220 nm with a high zeta value of +38 ± 2 mV led them to accumulate in cancer cells. Laser treatment did not affect the morphology of PP-IR NPs as observed under the transmission electron microscope (TEM). In vitro cytotoxicity studies on MCF-7 cells showed enhanced toxicity upon laser treatment. Further, we validated the cell death by reactive oxygen species (ROS) production. Our studies thus showed that PP-IR NPs are effective in suppressing metastatic cancer as the combinational therapy leads to the formation of apoptotic bodies in MCF-7 cells.

Nanoparticle‐Mediated Immunogenic Cell Death Enables and Potentiates Cancer Immunotherapy

“…[42] Owing to its immunostimulatory effect, glycated chitosan (GC) can be used as both an immunoadjuvant and acarrier to enhance the uptake and presentation of tumor antigens,i ncrease tumor immunogenicity,a nd synergize with PTT.K umar and Srivastava developed biocompatible and biodegradable monodisperse polycaprolactone/GC/Poloxamer blend NPs encapsulating photothermal agent IR 820 for imaging and photo-immunotherapy.Though the NPs enhanced toxicity on MCF-7 cells upon laser treatment, the immune response and efficacyinvivo were not investigated. [43] Angewandte Chemie As ap otent stimulator of the immune system, PTT has also shown synergy with ICB to reverse immunosuppression. Wang et al demonstrated the first combination of anti-CTLA-4 therapy with PTT using single-wall carbon nanotubes.T his stimulated DC maturation in tumor-draining lymph nodes (TDLNs), induced infiltration of effector Tcells, and greatly abrogated Treg cells in non-heated distant tumors, thereby reducing tumor burden in both subcutaneous and lung metastasis models and leading to prolonged survival.…”

IR820‐loaded PLGA nanoparticles for photothermal therapy of triple‐negative breast cancer

“…49 This was an exciting finding, but the complexity of this NP formulation may pose a significant barrier to its clinical translation. 21,48,49,51 In this work, we have developed biodegradable, polymeric NPs comprised of poly(lactic-co-glycolic acid) (PLGA) loaded with the NIR-absorbing dye IR820 (IR820-PLGA NPs) to enable proapoptotic PTT of TNBC. 51 They found that PTT mediated by their NPs induced a substantial amount of cell death, but the amount of cell death was only~10% greater than that induced by the NPs without light irradiation, which provide strictly doxorubicin delivery.…”

Multifunctional Conjugated Polymer Nanoparticles for Image‐Guided Photodynamic and Photothermal Therapy

“…Another strategy is to load PSs into targeted delivery nano‐carriers such as liposomes or polymer nanoparticles . Due to the aggregation‐caused quenching (ACQ) effect, traditional PSs generally show dramatically reduced ROS generation after being encapsulated into nanoparticles, compromising the final therapy performance.…”