Ionic boundaries in biological capillary electrophoresis

Foret, František; Klepárnı́k, Karel; Gebauer, Petr; Boček, Petr

doi:10.1016/s0021-9673(04)01428-1

Cited by 9 publications

(3 citation statements)

References 100 publications

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“…Transient ITP contributed to 15-20-fold sensitivity enhancement in the determination of both reduced and oxidized forms of glutathione in the diabetic nephropathy patient's plasma [49]. The use of ITP as well as other types of ionic boundaries for peptide and protein preconcentration has been reviewed by Foret et al [50].…”

Section: Preconcentration and Preseparationmentioning

confidence: 99%

Recent developments in capillary electrophoresis and capillary electrochromatography of peptides

2006

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The article gives a comprehensive review on the recent developments in the applications of high-performance capillary electromigration methods, zone electrophoresis, isotachophoresis, isoelectric focusing, affinity electrophoresis, electrokinetic chromatography, and electrochromatography, to analysis, preparation, and physicochemical characterization of peptides. The article presents new approaches to the theoretical description and experimental verification of electromigration behavior of peptides, covers the methodological aspects of capillary electroseparations of peptides, such as rational selection of separation conditions, sample preparation, suppression of peptide adsorption, new developments in individual separation modes, and new designs of detection systems. Several types of applications of capillary electromigration methods to peptide analysis are presented: conventional qualitative and quantitative analysis, purity control, determination in biomatrices, monitoring of chemical and enzymatical reactions and physical changes, amino acid and sequence analysis, and peptide mapping of proteins. Some examples of micropreparative peptide separations are given and capabilities of capillary electromigration techniques to provide important physicochemical characteristics of peptides are demonstrated.

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Section: Preconcentration and Preseparationmentioning

confidence: 99%

Recent developments in capillary electrophoresis and capillary electrochromatography of peptides

2006

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“…ITP is another preconcentration method based on electrokinetic phenomena. Compared to FESS and FESI where only the front boundary is stabilized, the sample is here sandwiched between two different BGEs that allow the stabilization of both sample plug boundaries [9,10].…”

Section: Introductionmentioning

confidence: 99%

Electrokinetic supercharging for highly efficient peptide preconcentration in capillary zone electrophoresis

2008

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Electrokinetic supercharging has been integrated in CZE for the development of a highly sensitive methodology for protein tryptic digest analysis. A careful choice of the experimental conditions led to sensitivity enhancement factors between 1000 and 10 000 whilst maintaining a satisfactory resolution. Peptides in the low nanomolar concentration range have been detected despite the use of the poorly sensitive UV absorbance detection mode. The buffer system used in this study is fully suitable for coupling CE to MS.

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“…Sheath liquid effect has been studied by Karger and co-workers and is often observed in a system with low EOF particularly when the ions in sheath liquid and running buffer have different mobility. In the current situation, because the EOF is low and phosphate ions have a higher effective mobility (234.1610 -9 m 2 /Vs) than acetate ions (23.1610 -9 m 2 /Vs), the conductivity of the newly formed zone would be lower than that of the original one causing the formation of a sharp ionic boundary [33]. A similar phenomenon (data not shown) was observed when 0.1% formic acid instead of 1% acetic acid was added to the sheath liquid.…”

Section: Analysis Of Antihistamines Using Ammonium Phosphate As the Rmentioning

confidence: 88%

CE‐MS of antihistamines using nonvolatile phosphate buffer

Chien

Huang

et al. 2007

Electrophoresis

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Antihistamines were analyzed by CE-ESI-MS using phosphate buffer. The separation was performed in an acidic environment so that phosphate ions had a net velocity flowing toward the inlet reservoir instead of the ESI source. To further reduce the effect of ion suppression, the sodium ion in sodium phosphate was replaced with an ammonium ion. Furthermore, with the combination of reducing the concentration of acid added to the sheath liquid and the use of a low-flow interface, phosphoric acid could be added to the sheath liquid. Because of the use of the same counterion (phosphate ion) in running buffer and in sheath liquid, the separation integrity (resolution, elution order, and peak shape) was preserved. In addition, ion suppression was also greatly alleviated because a minimal amount of phosphate flowed into the ESI source.

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Ionic boundaries in biological capillary electrophoresis

Cited by 9 publications

References 100 publications

Recent developments in capillary electrophoresis and capillary electrochromatography of peptides

Recent developments in capillary electrophoresis and capillary electrochromatography of peptides

Electrokinetic supercharging for highly efficient peptide preconcentration in capillary zone electrophoresis

CE‐MS of antihistamines using nonvolatile phosphate buffer

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