Ion‐pair chromatography with UV absorbing counterions on reversed‐phase layers. Part 1: Chromatography with hydroxynaphthalenesulfonic acids

Abstract

SummaryThe results of ion-pair chromatography with UV-absorbing counterions are described. Hydroxynaphthalenesulfonic acids are used as ion pair formers, and alkaloids as test samples. The separationsare performed on silanized silica gel from Merck and KCIBF plates from Whatman. The influence of the counterion on the Rf-value is demonstrated. Some alkaloids exhibit characteristic fluorescence as ion pairs.

Effect of mobile phase buffer pH on separation selectivity of some isoquinoline alkaloids in reversed-phase systems of Pressuriz

“…Thin Layer Chro ma tog ra phy (TLC) is very of ten ap plied as a tool for sepa ra tion of al ka loids. Vari ous al ka loids were de ter mined by RP-TLC sys tems, with buff ered aque ous elu ents [10,11,13,15,16], ion-pair rea gents [15,16,21,22], or chi ral se lec tors [4]. RP-TLC was used for de termi na tion of the lipo philicity of some al ka loids [23].…”