Reversed-Phase Ion-Pair Thin Layer Chromatography of Some Alkaloids

Shalaby, Abdalla; Khalil, Hawa M.

doi:10.1081/jlc-100101806

Cited by 6 publications

(5 citation statements)

References 25 publications

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“…Studying of the optimum parameters for maximum separation was carried out by investigating the effect of different variables. Different developing systems with different compositions and ratios were tried, but complete separation of HBB and PAR was achieved by using the reported developing mobile phase [25] consisting of methanol: citrate buffer (pH=1.5): triflouroacetic acid (70.0: 29.9: 0.1, by volume) .Also different scanning wavelengths (210, 230, 254 nm) were tested, but the best sensitivity obtained when 210 nm was used as scanning wavelength.…”

Section: Methods Development and Optimizationmentioning

confidence: 99%

“…Tocopherol acetate [24], Scopolamine Hydro Bromide [25] and mixture of Dipyrone and Hyoscine N-ButylBromide [26].…”

Section: Molecular Formula C21h30brno4mentioning

confidence: 99%

“…A space of 1 cm was left between each band and1.5 cm from the bottom edge of the plate. The plate was developed in a previously saturated chromatographic tank for one hour with the developing mobile phase consisting of methanol: citrate buffer (pH=1.5): triflouroacetic acid (70.0:29.9:0.1, by volume) by ascending chromatography at room temperature [25].…”

Section: Linearity and Construction Of Calibration Curvesmentioning

confidence: 99%

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Simultaneous Determination of Hyoscine N- Butyl Bromide and Paracetamol by RP-TLC Spectrodensitometric Method

Ali

Gamal

Abdelkawy

2013

BJPR

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Aims: A simple RP-TLC Spectrodensitometric method was developed for determination of Hyoscine N-Butyl Bromide (HBB) and Paracetamol (PAR) either in bulk powder or in their pharmaceutical preparation. Study Design: Validation study. Methodology: In this method, HBB and PAR were separated on RP-18 W/ UV 254 TLC plates using developing mobile phase consisting of methanol: citrate buffer (pH=1.5): triflouroacetic acid (70:30:0.1, by volume) at room temperature. Experimental conditions such as band size, slit width, different developing systems and scanning wavelength were carefully studied and the optimum conditions were selected. The obtained bands were then scanned at 210 nm. The two drugs were satisfactorily resolved with R F 0.60 ± 0.02 for HBB and 0.81 ± 0.02 for PAR. The validation of spectrodensitometric method was done regarding linearity, accuracy, precision, and specificity. Results: Linearity of the proposed methods was evaluated and it was found to lie within the concentration range of 2.0-12.0 µg.band -1 for HBB and 2.0-14.0 µg.band -1 for PAR.

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Section: Methods Development and Optimizationmentioning

confidence: 99%

“…Tocopherol acetate [24], Scopolamine Hydro Bromide [25] and mixture of Dipyrone and Hyoscine N-ButylBromide [26].…”

Section: Molecular Formula C21h30brno4mentioning

confidence: 99%

Section: Linearity and Construction Of Calibration Curvesmentioning

confidence: 99%

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Simultaneous Determination of Hyoscine N- Butyl Bromide and Paracetamol by RP-TLC Spectrodensitometric Method

Ali

Gamal

Abdelkawy

2013

BJPR

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“…Thin Layer Chro ma tog ra phy (TLC) is very of ten ap plied as a tool for sepa ra tion of al ka loids. Vari ous al ka loids were de ter mined by RP-TLC sys tems, with buff ered aque ous elu ents [10,11,13,15,16], ion-pair rea gents [15,16,21,22], or chi ral se lec tors [4]. RP-TLC was used for de termi na tion of the lipo philicity of some al ka loids [23].…”

Section: Introductionmentioning

confidence: 99%

Effect of mobile phase buffer pH on separation selectivity of some isoquinoline alkaloids in reversed-phase systems of Pressuriz

Kopciał¹,

Polak²,

Pietraś³

et al. 2015

Curr Issues Pharm Med Sci

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Separation of some isoquinoline alkaloids (narcotine, chelidonine, dihydrocodeine, cinchonine, berberine, cinchonidine, papaverine, apomorphine) has been investigated with pressurized planar electrochromatography (PPEC) and high-performance thin-layer chromatography (HPTLC) in reversed-phase systems. The mobile phase consisted of acetonitrile and aqueous buffer (disodium phosphate and citric acid). The influence of the mobile phase buffer pH on migration distance (PPEC) and retardation factor (HPTLC) of the solutes has been investigated and compared. The results show different separation selectivity in both PPEC and HPTLC systems especially at pH range of buffer solution of the mobile phase that facilitates ionization of the solutes investigated.

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“…[ 21 ] Shalaby and Khalil further modified this technique using an RP chromatographic plate with ion separation for the estimation of more alkaloids derived from plant sources. [ 22 ] Saqui-Sannes, et al adapted this method for the estimation of strychnine and crimidine in biological samples as well. [ 23 ] They determined the amounts of these alkaloids in dog stomach and serum, which are generally used as dog poisons.…”

mentioning

confidence: 99%

Simultaneous HPTLC determination of strychnine and brucine in strychnos nux-vomica seed

Kamal

Ahmad

et al. 2012

J Pharm Bioall Sci

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Objective:A simple, sensitive, and specific thin layer chromatography (TLC) densitometry method has been developed for the simultaneous quantification of strychnine and brucine in the seeds of Strychnos nux-vomica.Materials and Methods:The method involved simultaneous estimation of strychnine and brucine after resolving it by high performance TLC (HPTLC) on silica gel plate with chloroform–methanol–formic acid (8.5:1.5:0.4 v/v/v) as the mobile phase.Results:The method was validated as per the ICH guidelines for precision (interday, intraday, intersystem), robustness, accuracy, limit of detection, and limit of quantitation. The relationship between the concentration of standard solutions and the peak response was linear within the concentration range of 50–1000 ng/spot for strychnine and 100–1000 ng/spot for brucine. The method precision was found to be 0.58–2.47 (% relative standard deviation [RSD]) and 0.36–2.22 (% RSD) for strychnine and brucine, respectively. Accuracy of the method was checked by recovery studies conducted at three different concentration levels and the average percentage recovery was found to be 100.75% for strychnine and 100.52% for brucine, respectively.Conclusions:The HPTLC method for the simultaneous quantification of strychnine and brucine was found to be simple, precise, specific, sensitive, and accurate and can be used for routine analysis and quality control of raw material of S. nux-vomica and several unani and ayurvedic formulations containing this as an ingredient.

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Reversed-Phase Ion-Pair Thin Layer Chromatography of Some Alkaloids

Cited by 6 publications

References 25 publications

Simultaneous Determination of Hyoscine N- Butyl Bromide and Paracetamol by RP-TLC Spectrodensitometric Method

Simultaneous Determination of Hyoscine N- Butyl Bromide and Paracetamol by RP-TLC Spectrodensitometric Method

Effect of mobile phase buffer pH on separation selectivity of some isoquinoline alkaloids in reversed-phase systems of Pressuriz

Simultaneous HPTLC determination of strychnine and brucine in strychnos nux-vomica seed

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