Ion implantation of organisms

Feng, Hao; Zhang, Yu; Chu, Paul K.

doi:10.1016/j.mser.2006.11.001

Cited by 82 publications

(61 citation statements)

References 214 publications

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“…and exposure time under vacuum environment. However, only the various doses of ions implanted in the cells can cause gene mutation [17,21]. Figure 1 showed the survive rate of the cells exposed to different energy of N ?…”

Section: Resultsmentioning

confidence: 99%

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Mutation-Screening in l-(+)-Lactic Acid Producing Strains by Ion Implantation

Zhaoyang

et al. 2011

Indian J Microbiol

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In this paper, in order to obtain some industrial strains with high yield of L-(?)-lactic acid, the wild type strain Lactobacillus casei CICC6028 was mutated by nitrogen ions implantation. By study, it was found that the high positive mutation rate was obtained when the output power was 10 keV and the dose of N ? implantation was 50 9 2.6 9 10 13 ions/cm 2 . In addition, the initial screening methods were also studied, and it was found that the transparent halos method was unavailable, for some high yield strains of L-(?)-lactic acid were missed. Then a mutant strain which was named as N-2 was isolated, its optimum fermentation temperature was 40°C and the L-(?)-lactic acid yield was 136 g/l compared to the original strain whose optimum fermentation temperature was 34°C and L-(?)-lactic acid production was 98 g/l. Finally, High Performance Liquid Chromatography method was used to analyze the purity of L-(?)-lactic acid that was produced by the mutant N-2, and the result showed the main production of N-2 was L-(?)-lactic acid.

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Section: Resultsmentioning

confidence: 99%

“…This was just the characteristic survival curve of N ? implantation [21,23]. Figure 4 showed the relationship between dosedependent survival rate and mutation rate, when cells were exposed to the various doses of N ?…”

Section: Resultsmentioning

confidence: 99%

Mutation-Screening in l-(+)-Lactic Acid Producing Strains by Ion Implantation

Zhaoyang

et al. 2011

Indian J Microbiol

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“…[16] In addition, the introduction of foreign genetic material into yeasts by ion implantation is a random process, which generates a variety of individual transformants containing one to several genes involved in secondary metabolite biosynthesis or its regulatory genes in random or tandem arrangements after transformation. These features pose potential complications in the high-efficiency selection of the target recombinant yeast.…”

Section: Screening and Identification Of The Gentiopicrosideproducingmentioning

confidence: 99%

“…This phenomenon might be explained either by yeast alternative splicing, [23] or by the biological effects induced by ion implantation, including energy absorption, mass deposition and charge transfer of energetic ions in organisms. [16] A deeper insight into the molecular mechanism by which plant genes were correctly spliced in recombinant yeasts obtained by ion implantation is required to validate the method, which is currently under investigation.…”

Section: Expression Pattern Of G10h and Sls Genes Of Dl67 During Growthmentioning

confidence: 99%

“…[16] In 1988, the etching effect of ion beams on the cell wall was discovered, which subsequently led to the rapid development of low-energy ion-beam-induced exogenous macromolecule transfer into biological cells. [17] In this study, the ion beam-mediated gene transfer method was used to introduce genomic DNA segments of the medicinal plant G. macrophylla into Hansenula polymorpha to achieve single-step whole-pathway assembly of gentiopicroside biosynthesis in yeasts.…”

Section: Introductionmentioning

confidence: 99%

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Engineering of gentiopicroside-producing yeast strain using low-energy ion implantation mediated synthetic biology

Wang

Qian

et al. 2016

Biotechnology & Biotechnological Equipment

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To obtain an alternative source for the production of gentiopicroside, here genomic DNA segments of the medicinal plant Gentiana macrophylla were randomly transferred into Hansenula polymorpha by 25 KeV nitrogen ions (N C ) at a dose of 2.5 £ 10 16 ions/cm 2 under vacuum pressure of 1 £ 10 ¡3Pa. To screen for potential gentiopicroside-producing recombinant yeast strains, geraniol 10-hydroxylase (G10H) and secologanin synthase (SLS) involved in the gentiopicroside biosynthesis pathway were used as molecular markers. Based on the conserved protein sequences of G10H and SLS, degenerate primers were designed and used for colony polymerase chain reaction (PCR). PCRpositive results for both the G10H and SLS genes were obtained in 79 out of 653 transformants by low-energy ion beam-mediated transformation. These 79 potential gentiopicroside-producing strains were further analysed by Fehling's test, thin-layer chromatography, high performance liquid chromatography and high performance liquid chromatography-mass spectrometry. The results showed that the retention time and ion peaks of the sample from one stable recombinant strain designated as DL67 were consistent with those of the gentiopicroside standard. The corresponding gentiopicroside yield was 8.41 mg/g dry cell weight after strain DL67 was cultured for 96 h. This could offer a new starting point for the construction of recombinant yeasts for production of medicinal plant compounds.

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Microbial Lipid Production by Co‐Fermentation with Mortierella alpina Obtained by Ion Beam Implantation

et al. 2011

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Co-fermentation of lignocellulose-based carbohydrates is a potential approach to improve the economics of microbial lipid production. To obtain an industrial strain that metabolizes glucose and xylose with a higher microbial lipid production, the strain Mortierella alpina was mutated by low-energy ion beam implantation. After nitrogen ion beam implantation, a mutant strain, M. alpina I502-8 (MAI502-8), was obtained. MAI502-8 was an efficient producer of microbial lipid from glucose and xylose (5:3 wt/wt). Moreover, the co-fermentation conditions were also optimized. Under optimal conditions, the maximum yield of biomass and microbial lipid reached 27.4 and 10.05 g L -1, respectively, which were 2.38-fold and 1.84-fold those in the initial media. Of the mixed sugar, 84.33 % was used up during co-fermentation.

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Ion implantation of organisms

Cited by 82 publications

References 214 publications

Mutation-Screening in l-(+)-Lactic Acid Producing Strains by Ion Implantation

Mutation-Screening in l-(+)-Lactic Acid Producing Strains by Ion Implantation

Engineering of gentiopicroside-producing yeast strain using low-energy ion implantation mediated synthetic biology

Microbial Lipid Production by Co‐Fermentation with Mortierella alpina Obtained by Ion Beam Implantation

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