Microbial Lipid Production by Co‐Fermentation with Mortierella alpina Obtained by Ion Beam Implantation

Abstract

Co-fermentation of lignocellulose-based carbohydrates is a potential approach to improve the economics of microbial lipid production. To obtain an industrial strain that metabolizes glucose and xylose with a higher microbial lipid production, the strain Mortierella alpina was mutated by low-energy ion beam implantation. After nitrogen ion beam implantation, a mutant strain, M. alpina I502-8 (MAI502-8), was obtained. MAI502-8 was an efficient producer of microbial lipid from glucose and xylose (5:3 wt/wt). Moreover, the co-fermentation conditions were also optimized. Under optimal conditions, the maximum yield of biomass and microbial lipid reached 27.4 and 10.05 g L -1, respectively, which were 2.38-fold and 1.84-fold those in the initial media. Of the mixed sugar, 84.33 % was used up during co-fermentation.

Screening, identification, and low‐energy ion modified breeding of a yeast strain producing high level of 3‐hydroxypropionic acid

“…Cordyceps militaris strains containing abundant Selenium were obtained by ion implantation (Wang, Li, Chen, Li, & Zhao, ). Low‐energy ion can be applied to breed different kinds of bacteria that produce D(–)‐lactic acid, vitamin C, rifamycin, tannase, microbial lipid, and arachidonic acid, separately (Chen et al, ; Nie et al, ; Wang, Zhang, Zheng, & Wang, ; Xu, Bai, Wang, & He, ; Yu et al, ; Yu, Xu, Wang, & Yu, ).…”

“…Low-energy ion can be applied to breed different kinds of bacteria that produce D(-)-lactic acid, vitamin C, rifamycin, tannase, microbial lipid, and arachidonic acid, separately (Chen et al, 1998;Nie et al, 2013;Wang, Zhang, Zheng, & Wang, 2011;Xu, Bai, Wang, & He, 2010;Yu et al, 2012;Yu, Xu, Wang, & Yu, 1999). This work aims to breed strains that can produce high titer of 3HP.…”