The development of bio-based resources has become an important strategy to improve the security of energy resources, reduce green house gas emissions, and address climate change . 3-Hydroxypropionic acid (3HP) is an significant chemical intermediate that contains two functional groups, namely, hydroxyl and carboxyl (Ko, Ashok, Zhou, Kumar, & Park, 2012). 3HP, as the precursor of many optically active substances and an important platform chemical, directly affects the production of many high value-added chemicals. 3HP can be used to produce malonic acid, 1,3-propanediol, succinic acid, special polyester, and acrylicacid (Ashok et al., 2013;Henry, Broadbelt, & Hatzimanikatis, 2010;Zheng, Zhang, Zhang, & Chen, 2004). 3HP can also be used to generate various critical fine chemical products. Currently, 3HP is a chemical product with high potential for development by the US Department of Energy (Jiang, Meng, & Xian, 2009). 3HP is traditionally prepared through chemical synthesis. For example, it is produced by the reaction between adjacent halogenated glycol and potassium cyanide through hydrolysis or Reformatsky reaction (Ishida & Ueno, 2000). Chemical synthesis for 3HP production is difficult and expensive. The corresponding separation and purification procedures are also complicated.Biological methods can effectively avoid the limitations of chemical synthesis and possess low cost, mild conditions, and other advantages. Up to now, several organisms have been reported to produce 3-HP from at least six compounds: glycerol (Ashok, Raj,