Involvement of small G protein RhoB in the regulation of proliferation, adhesion and migration by dexamethasone in osteoblastic cells

Diao, Fei; Chen, Kangyao; Wang, Yan; Li, Yidong; Xu, Weidong; Lü, Jing; Chen, Yuxia

doi:10.1371/journal.pone.0174273

Cited by 4 publications

(2 citation statements)

References 45 publications

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“…We also showed that RhoB was downregulated in osteosarcoma tissues and low expression of RhoB predicted poor prognosis of osteosarcoma patients. Furthermore, in lung cancer and osteoblastic cell RhoB mediated cell proliferation, adhesion, migration and invasion in lung cancer and osteoblastic cell 27,28. In our study, knockdown of miR-19a inhibited osteosarcoma cell EMT through RhoB.…”

Section: Discussionsupporting

confidence: 58%

“…RhoB, usually acted as a tumor suppressor, which was associated with multiple cellular progresses, including cell growth, adhesion and transformation 23–25. In lung cancer, RhoB regulated the activity of PP2A to modulate mesenchymal phenotype and invasion 26,27. Diao et al28 discovered that RhoB mediated cell proliferation, adhesion and migration in osteoblastic cells.…”

Section: Introductionmentioning

confidence: 99%

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<p>MiR-19a Promotes Migration And Invasion By Targeting RHOB In Osteosarcoma</p>

Liu¹,

Shen²,

Qu³

et al. 2019

OTT

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IntroductionOsteosarcoma is the most common bone tumor with high metastasis and recurrence rate. MicroRNA-19a (miR-19a) has been reported to act as tumor oncogene in multiple cancers. The objective of the study was to explore the molecular mechanisms of miR-19a in osteosarcoma cell migration and invasion.Materials and methodsReal-time quantitative polymerase chain reaction (RT-qPCR) and Western blotting were employed to measure the levels of miR-19a and RhoB in osteosarcoma tissues and cell lines. Transwell assay was employed to analyze the tissues and cell lines’ migratory and invasive abilities. Dual luciferase reporter assay was utilized to analyze the association between miR-19a and RhoB.ResultsMiR-19a was overexpressed in osteosarcoma tissues and cell lines. MiR-19a promoted osteosarcoma cell migration and invasion in vitro. RhoB was thus confirmed as a direct and functional target of miR-19a, and it could partially reverse the function of miR-19a. Knockdown miR-19a inhibited osteosarcoma cell epithelial-mesenchymal transition (EMT) and suppressed osteosarcoma xenograft growth.ConclusionMiR-19a enhanced cell migration, invasion and EMT through RhoB in osteosarcoma. The newly identified miR-19a/RhoB axis provides novel insight into the progression of osteosarcoma and offers a promising target for osteosarcoma therapy.

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Section: Discussionsupporting

confidence: 58%

Section: Introductionmentioning

confidence: 99%

<p>MiR-19a Promotes Migration And Invasion By Targeting RHOB In Osteosarcoma</p>

Liu¹,

Shen²,

Qu³

et al. 2019

OTT

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ARAP1 negatively regulates stress fibers formation and metastasis in lung adenocarcinoma via controlling Rho signaling

Zhang,

Xie,

Gong

et al. 2023

Discov Onc

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Small GTPases regulate multiple important cellular behaviors and their activities are strictly controlled by a mass of regulators. The dysfunction or abnormal expression of small GTPases or their regulators was frequently observed in various cancers. Here, we analyzed the expression and prognostic correlation of several GTPases and related regulators based on the TCGA database and found that Ankyrin Repeat and PH Domain 1 (ARAP1), a GTPase activating protein (GAP), is reduced in lung adenocarcinoma tissues compared to normal tissues and displays a positive correlation with overall survival (OS) and progression-free survival (PFS) of patients with lung adenocarcinoma. qPCR and western blot verified that ARAP1 is frequently downregulated in lung adenocarcinoma tumor tissues and cancer cells, and its downregulation might be mediated by epigenetic modification. Moreover, metastatic assays showed that overexpression of ARAP1 significantly inhibits metastasis of lung adenocarcinoma in vitro and in vivo. We further demonstrated that Rho signaling inhibition, mediated by RhoGAP activity of ARAP1, majorly contributes to suppressing migration and invasion of lung adenocarcinoma cancer cells via inhibiting stress fibers formation. In summary, this study indicates that ARAP1 may serve as a potential prognostic predictor and a metastatic suppressor in lung adenocarcinoma via its RhoGAP activity.

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From epidemiology and neurodevelopment to antineoplasticity. Medroxyprogesterone reduces human glial tumor growth in vitro and C6 glioma in rat brain in vivo

Altinöz

Nalbantoglu

Özpınar

et al. 2018

Clinical Neurology and Neurosurgery

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Involvement of small G protein RhoB in the regulation of proliferation, adhesion and migration by dexamethasone in osteoblastic cells

Cited by 4 publications

References 45 publications

<p>MiR-19a Promotes Migration And Invasion By Targeting RHOB In Osteosarcoma</p>

<p>MiR-19a Promotes Migration And Invasion By Targeting RHOB In Osteosarcoma</p>

ARAP1 negatively regulates stress fibers formation and metastasis in lung adenocarcinoma via controlling Rho signaling

From epidemiology and neurodevelopment to antineoplasticity. Medroxyprogesterone reduces human glial tumor growth in vitro and C6 glioma in rat brain in vivo

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