<p>MiR-19a Promotes Migration And Invasion By Targeting RHOB In Osteosarcoma</p>

Abstract

IntroductionOsteosarcoma is the most common bone tumor with high metastasis and recurrence rate. MicroRNA-19a (miR-19a) has been reported to act as tumor oncogene in multiple cancers. The objective of the study was to explore the molecular mechanisms of miR-19a in osteosarcoma cell migration and invasion.Materials and methodsReal-time quantitative polymerase chain reaction (RT-qPCR) and Western blotting were employed to measure the levels of miR-19a and RhoB in osteosarcoma tissues and cell lines. Transwell assay was employed to analyze the tissues and cell lines’ migratory and invasive abilities. Dual luciferase reporter assay was utilized to analyze the association between miR-19a and RhoB.ResultsMiR-19a was overexpressed in osteosarcoma tissues and cell lines. MiR-19a promoted osteosarcoma cell migration and invasion in vitro. RhoB was thus confirmed as a direct and functional target of miR-19a, and it could partially reverse the function of miR-19a. Knockdown miR-19a inhibited osteosarcoma cell epithelial-mesenchymal transition (EMT) and suppressed osteosarcoma xenograft growth.ConclusionMiR-19a enhanced cell migration, invasion and EMT through RhoB in osteosarcoma. The newly identified miR-19a/RhoB axis provides novel insight into the progression of osteosarcoma and offers a promising target for osteosarcoma therapy.

<p>Long Noncoding RNA LINC00839 Promotes the Malignant Progression of Osteosarcoma by Competitively Binding to MicroRNA-454-3p and Consequently Increasing c-Met Expression</p>

“…In total, 27 miRNAs were simultaneously identified from the two data sets. MiR-106b-5p, 24 miR-130a-3p, 25 miR-130b-3p, 26 miR-17-5p, 27 miR-19a-3p, 28 miR-19b-3p, 29 miR-301a-3p, 30 miR-4295, 31 and miR-93-5p 32 have been previously shown to be upregulated in OS and play oncogenic roles during cancer progression. In addition, no studies have reported an association of miR-1180-5p, miR-301b-3p, miR-3666, miR-519b-3p, miR-519c-3p, miR-520g-3p, miR-520h, miR-5590-3p, and miR-7114-3p with OS.…”

Mesenchymal stem cells paracrine proteins from three‐dimensional dynamic culture system promoted wound healing in third‐degree burn models

“…), and even cause excessive inflammatory response (such as CD47, CCL2, etc.). 34,35 Although there are only 465 protein species in the 3D group, among which these proteins can specifically promote the mediation of cell attachment and migration (such as LAMA1, POSTN, TLN1, etc. ), promote cell proliferation (such as PRKDC, ASCC3, MYH10, PDK1, etc.…”