Involvement of c-Myc in the proliferation of MCF-7 human breast cancer cells induced by bHLH transcription factor DEC2

Wu, Yunyan; Sato, Hidenobu; Suzuki, Tsuyoshi; Yoshizawa, Tadashi; Morohashi, Satoko; Seino, Hiroko; Kawamoto, Takeshi; Fujimoto, Katsumi; Kato, Yukio; Kijima, Hiroshi

doi:10.3892/ijmm.2014.2042

Cited by 25 publications

(25 citation statements)

References 40 publications

(50 reference statements)

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“…The expression of growth differentiation factor 15 (GDF15), a member of the transforming growth factor beta (TGFβ) superfamily, and interleukin-32 (IL-32), is known to induce apoptosis (Park, et al 2012; Podar, et al 2007; Wang, et al 2012; Yun, et al 2013), while TMPRSS2 is a known AR target suppressed in CRPC. Heme oxygenase 1 (HMOX1) counteracts oxidative and inflammatory damage and is implicated in the adhesive and morphological properties of tumor cells (Gueron, et al 2014), while Basic loop-helix-loop E40 (BHLHE40) is a transcription factor is involved in the regulation of cell differentiation, response to hypoxia and carcinogenesis (Wu, et al 2014). qPCR showed that each of these genes were under-expressed in C4-2 compared to LNCaP, while expression of nuclear FlnA restores their expression (Figure 7D).…”

Section: Resultsmentioning

confidence: 99%

Transcription of Nrdp1 by the androgen receptor is regulated by nuclear filamin A in prostate cancer

Savoy¹,

Chen²,

Siddiqui³

et al. 2015

Endocrine-Related Cancer

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Prostate cancer (PCa) progression is regulated by the androgen receptor (AR); however, patients undergoing androgen deprivation therapy (ADT) for disseminated PCa eventually develop castration resistant PCa (CRPC). Studies showed that AR, a transcription factor, occupies distinct genomic loci in CRPC compared to hormone-naïve PCa; however, the cause for this distinction was unknown. The E3 ubiquitin ligase Nrdp1 is a model AR target modulated by androgens in hormone-naïve PCa but not in CRPC. Using Nrdp1, we investigated how AR switches transcription programs during CRPC progression. The proximal Nrdp1 promoter contains an androgen response element (ARE); we demonstrated AR binding to this ARE in androgen-sensitive PCa. Analysis of hormone-naive human prostatectomy specimens revealed correlation between Nrdp1 and AR expression, supporting AR regulation of Nrdp1 levels in androgen-sensitive tissue. However, despite sustained AR levels, AR binding to the Nrdp1 promoter and Nrdp1 expression were suppressed in CRPC. Elucidation of the suppression mechanism demonstrated correlation of Nrdp1 levels with nuclear localization of the scaffolding protein Filamin A (FlnA) which, as we previously showed, is itself repressed following ADT in many CRPC tumors. Restoration of nuclear FlnA in CRPC stimulated AR binding to Nrdp1 ARE, increased its transcription, and augmented Nrdp1 protein expression and responsiveness to ADT, indicating that nuclear FlnA controls AR-mediated androgen-sensitive Nrdp1 transcription. Expressions of other AR-regulated genes lost in CRPC were also re-established by nuclear FlnA. Thus our data demonstrate that nuclear FlnA promotes androgen-dependent AR-regulated transcription in PCa, while loss of nuclear FlnA in CRPC alters the AR-regulated transcription program.

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Section: Resultsmentioning

confidence: 99%

Transcription of Nrdp1 by the androgen receptor is regulated by nuclear filamin A in prostate cancer

Savoy¹,

Chen²,

Siddiqui³

et al. 2015

Endocrine-Related Cancer

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“…As effectors of hypoxia, DEC1 response was more rapid to hypoxic stress than DEC2. Previous findings showed that the upregulation of DEC2 in the latter stage of hypoxia accompanied with the increase in cell proliferation led to oxygen-deprived stress (19). …”

Section: Discussionmentioning

confidence: 98%

“…Cell viability analysis was performed using the MTS [3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium] assay as described previously (19). …”

Section: Methodsmentioning

confidence: 99%

Basic helix-loop-helix transcription factor DEC2 functions as an anti-apoptotic factor during paclitaxel-induced apoptosis in human prostate cancer cells

Liu

Yoshizawa

et al. 2016

International Journal of Molecular Medicine

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The functions of basic helix-loop-helix (bHLH) transcription factor-differentiated embryonic chondrocyte (DEC)1 (BHLHE40) and 2 (BHLHE41) are involved in various fields such as circadian rhythms, immune responses, cell proliferation, hypoxia reaction as well as malignant tumors. Previous findings showed that DEC served as apoptosis regulators of various cancer cell lines. However, little is known regarding the expression of DEC1 and DEC2 in prostate cancer cells. The present study aimed to examine the roles of DEC1 and DEC2 in human prostate cancer DU145 and PC-3 cells that were treated with paclitaxel. The expression of DEC1 and DEC2 was decreased in DU145 cells but was increased in PC-3 cells when treated with paclitaxel. DU145 cells were more sensitive to paclitaxel than PC-3 cells since the amount of cleaved poly(ADP-ribose) polymerase (PARP) reached its peak at 50 μM of paclitaxel in DU145 cells but at 100 μM in PC-3 cells. In addition, the amount of cleaved PARP was decreased by DEC1 siRNA, while it was increased by DEC2 siRNA in the presence of paclitaxel. Although DEC2 overexpression slightly inhibited cleaved PARP in the two cell lines, the effects of DEC1 overexpression on apoptosis remain to be determined. In conclusion, DEC1, at least partly, exerted a pro-apoptotic effect, whereas DEC2 exerted an anti-apoptotic effect in paclitaxel-induced apoptosis of human prostate cancer cells.

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“…The cells were transfected with an empty plasmid (pcDNA) or the expression plasmids for DEC1 or DEC2 (DEC1 pcDNA or DEC2 pcDNA, respectively). Following 18 h of transfection, the cells were cultured with or without 40 µM cisplatin for another 24 h. Cell viability was assessed with the MTS assay, as previously described (16). …”

Section: Methodsmentioning

confidence: 99%

DEC2 expression antagonizes cisplatin-induced apoptosis in human esophageal squamous cell carcinoma

Sato

Kato

et al. 2017

Molecular Medicine Reports

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Differentiated embryonic chondrocyte expressed gene 1 (DEC1) and differentiated embryonic chondrocyte expressed gene 2 (DEC2) belong to the Hairy/Enhancer of Split subfamily of basic helix-loop-helix factors. Previous studies have demonstrated that DEC proteins are involved in the regulation of circadian rhythms, response to hypoxia, and tumorigenesis. However, the roles of DEC1 and DEC2 in apoptosis of esophageal carcinoma remain unclear. In the present study, alterations in expression of apoptosis-related markers in human esophageal squamous cell carcinoma TE-11 cells treated with cisplatin were examined by western blot, while overall cell viability and apoptosis were analyzed by MTS assay and hematoxylin and eosin staining, respectively. Following cisplatin treatment, expression of DEC2 was downregulated, whereas expression of DEC1 was upregulated. DEC2 overexpression during cisplatin treatment markedly inhibited expression of the pro-apoptotic factor Bim and slightly increased the anti-apoptotic factor Bcl-xL. However, overexpression of DEC1 during cisplatin treatment failed to affect expression of these markers. Additionally, overexpression of DEC2 improved cell viability and decreased cell apoptosis induced by cisplatin. These results suggested that DEC2 exhibits anti-apoptotic effects in TE-11 esophageal squamous cell carcinoma cells. Inhibiting DEC2 may therefore have therapeutic potential for the treatment of esophageal cancer, in combination with cisplatin.

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Involvement of c-Myc in the proliferation of MCF-7 human breast cancer cells induced by bHLH transcription factor DEC2

Cited by 25 publications

References 40 publications

Transcription of Nrdp1 by the androgen receptor is regulated by nuclear filamin A in prostate cancer

Transcription of Nrdp1 by the androgen receptor is regulated by nuclear filamin A in prostate cancer

Basic helix-loop-helix transcription factor DEC2 functions as an anti-apoptotic factor during paclitaxel-induced apoptosis in human prostate cancer cells

DEC2 expression antagonizes cisplatin-induced apoptosis in human esophageal squamous cell carcinoma

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