Invitro Bioactivity and Osteogenic Activity Study of Solid State Synthesized Nano‐Hydroxyapatite using Recycled Eggshell Bio–waste

Ingole, Vijay H.; Hussein, Kamal Hany; Kashale, Anil A.; Gattu, Ketan P.; Dhanayat, Swapnali; Vinchurkar, Aruna; Chang, Jia‐Yaw; Ghule, Anil V.

doi:10.1002/slct.201601092

Cited by 23 publications

(19 citation statements)

References 50 publications

(16 reference statements)

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“…OC and OP are osteoblast‐specific marker genes . OC is a major noncollagenous protein component of the bone extracellular matrix (ECM) which considered as a relatively late marker of osteoblast differentiation related to mineralization produced by both osteoblasts and osteoclasts.…”

Section: Resultsmentioning

confidence: 99%

“…In recent years, significant research effort has been devoted in developing the preparation and morphology control methods of HAp. HAp with various morphologies have been synthesized by means of solid‐state reaction, sol–gel method, template‐directed method, hydrothermal method, and microemulsion, or emulsion technique . Indeed, morphology and properties depend strongly on the synthesis approach and conditions used.…”

Section: Introductionmentioning

confidence: 99%

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Ultrasound‐assisted green economic synthesis of hydroxyapatite nanoparticles using eggshell biowaste and study of mechanical and biological properties for orthopedic applications

Ingole

Hussein

Kashale

et al. 2017

J Biomedical Materials Res

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Nanostructured hydroxyapatite (HAp) is the most favorable candidate biomaterial for bone tissue engineering because of its bioactive and osteoconductive properties. Herein, we report for the first time ultrasound-assisted facile and economic approach for the synthesis of nanocrystalline hydroxyapatite (Ca (PO ) (OH) ) using recycled eggshell biowaste referred as EHAp. The process involves the reaction of eggshell biowaste as a source of calcium and ammonium dihydrogen orthophosphate as a phosphate source. Ultrasound-mediated chemical synthesis of hydroxyapatite (HAp) is also carried out using similar approach wherein commercially available calcium hydroxide and ammonium dihydrogen orthophosphate were used as calcium and phosphate precursors, respectively and referred as CHAp for better comparison. The prepared materials were characterized by X-ray diffraction, field emission scanning electron microscopy, Fourier transform infrared spectroscopy, and Raman spectroscopy to determine crystal structure, particle morphology, and the presence of chemical functional groups. The nanocrystalline EHAp and CHAp were observed to have spherical morphology with uniform size distribution. Furthermore, mechanical properties such as Vickers hardness, fracture toughness, and compression tests have been studied of the EHAp and CHAp samples showing promising results. Mechanical properties show the influence of calcination at 600°C EHAp and CHAp material. After calcination, in the case of EHAp material an average hardness, mechanical strength, elastic modulus, and fracture toughness were found 552 MPa, 46.6 MPa, 2824 MPa, and 3.85 MPa m , respectively, while in the case of CHAp 618 MPa, 47.5 MPa, 2071 MPa, and 3.13 MPa m . In vitro cell studies revealed that the EHAp and CHAp nanoparticles significantly increased the attachment and proliferation of the hFOB cells. Here, we showed that EHAp and CHAp provide promising biocompatible materials that do not affect the cell viability and proliferation with enhancing the osteogenic activity of osteoblasts. Moreover, hFOB cells are found to express Osteocalcin, Osteopontin, Collagen I, Osteonectin, BMP-2 on the EHAp and CHAp bone graft. This study demonstrates the formation of pure nanocrystalline HAp with promising properties justifying the fact that the eggshell biowaste could be successfully used for the synthesis of HAp with good mechanical and osteogenic properties. These findings may have significant implications for designing of biomaterial for use in orthopedic tissue regeneration. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 2935-2947, 2017.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Ultrasound‐assisted green economic synthesis of hydroxyapatite nanoparticles using eggshell biowaste and study of mechanical and biological properties for orthopedic applications

Ingole

Hussein

Kashale

et al. 2017

J Biomedical Materials Res

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“…They are commonly used as bone grafts, illers and as coatings for metal implants. They are characterised for bone tissue engineering due to the properties such as biocompatibility, bioactivity and other mechanical properties without getting rejected by the immune systems (Ingole et al, 2016).…”

Section: Biomedical Approachmentioning

confidence: 99%

Synthesis of Hydroxyapatite Crystals From Egg Shells

George¹,

Somasundaram²,

S³

et al. 2020

ijrps

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The aim of the review is to raise awareness to synthesise hydroxyapatite (HAp) crystals from eggshells. The development of hydroxyapatite crystals shows a systematic approach towards the development in various biomedical applications. The economic approach of the synthesis of crystalline hydroxyapatite crystals from the eggshells is done using ultrasound assisted technology. The study demonstrates the use of biowastes such as egg shells which is a promising technique to develop hydroxyapatite crystals due to its mechanical and osteogenic properties. The hydroxyapatite crystals are synthesised using biowastes such as egg shells which are rich in calcium and ammonium dihydrogen phosphate. This is a viable substitute for bone regeneration and orthodontic appliances due to its properties such as fracture toughness, mechanical strength, elastic modulus, osteoconductivity, bioactivity and differentiation. These minerals are similar to the inorganic content of the bone and are used widely for complete or partial bone augmentation, filling bone and teeth. They are used as coatings in orthopaedics and dental implants. The organic components of the matrix of the egg shells are mainly composed of mucopolysaccharides, proteins, mainly composed of chondroitin sulphate A and B, glucosamine, galactosamine, galactose, mannose, fructose and sialic acid. They are widely used as the matrix fibres have a strong influence on the mechanical strength of the entire egg shells. These crystals are characterised for bone tissue engineering due to the following properties such as biocompatibility, bioactivity and other mechanical properties without getting rejected by the immune system.

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“…The preparation of CaO from the eggshell and its further conversion into Ca(OH) 2 , used as a calcium precursor for the preparation of the HA, was presented in our previous work [68]; the detailed preparation protocol was given in Supplementary Materials. TEMPO-mediated oxidation of the CNF was performed as previously reported elsewhere [40,69]; the detailed procedure was given in Supplementary Materials. In order to prepare the HA-TCNF/CNC nanocomposites with a varying TCNF/CNC composition, the prepared solutions of the precursors were mixed with 10, 20, 30, and 40 wt % of the TCNF and CNC suspensions, respectively, followed by their ultrasonication for 10 min to form homogeneous dispersions.…”

Section: Preparation Of the Ha-tcnf And Ha-cnc Nanocompositesmentioning

confidence: 99%

Mechanical Properties and Cytotoxicity of Differently Structured Nanocellulose-hydroxyapatite Based Composites for Bone Regeneration Application

et al. 2019

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The nanocomposites were prepared by synthesizing (2,2,6,6-tetramethylpiperidin-1-yl)oxyl (TEMPO)-oxidized cellulose nanofibrils (TCNFs) or cellulose nanocrystals (CNCs) with hydroxyapatite (HA) in varying composition ratios in situ. These nanocomposites were first obtained from eggshell-derived calcium and phosphate of ammonium dihydrogen orthophosphate as precursors at a stoichiometric Ca/P ratio of 1.67 with ultrasonication and compressed further by a uniaxial high-pressure technique. Different spectroscopic, microscopic, and thermogravimetric analyses were used to evaluate their structural, crystalline, and morphological properties, while their mechanical properties were assessed by an indentation method. The contents of TCNF and CNC were shown to render the formation of the HA crystallites and thus influenced strongly on the composite nanostructure and further on the mechanical properties. In this sense, the TCNF-based composites with relatively higher contents (30 and 40 wt %) of semicrystalline and flexible TCNFs resulted in smoother and more uniformly distributed HA particles with good interconnectivity, a hardness range of 550–640 MPa, a compression strength range of 110–180 MPa, an elastic modulus of ~5 GPa, and a fracture toughness value of ~6 MPa1/2 in the range of that of cortical bone. Furthermore, all the composites did not induce cytotoxicity to human bone-derived osteoblast cells but rather improved their viability, making them promising for bone tissue regeneration in load-bearing applications.

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Invitro Bioactivity and Osteogenic Activity Study of Solid State Synthesized Nano‐Hydroxyapatite using Recycled Eggshell Bio–waste

Cited by 23 publications

References 50 publications

Ultrasound‐assisted green economic synthesis of hydroxyapatite nanoparticles using eggshell biowaste and study of mechanical and biological properties for orthopedic applications

Ultrasound‐assisted green economic synthesis of hydroxyapatite nanoparticles using eggshell biowaste and study of mechanical and biological properties for orthopedic applications

Synthesis of Hydroxyapatite Crystals From Egg Shells

Mechanical Properties and Cytotoxicity of Differently Structured Nanocellulose-hydroxyapatite Based Composites for Bone Regeneration Application

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