Investigations on the Antifungal Effect of Nerol against Aspergillus flavus Causing Food Spoilage

Abstract

The antifungal efficacy of nerol (NEL) has been proved against Aspergillus flavus by using in vitro and in vivo tests. The mycelial growth of A. flavus was completely inhibited at concentrations of 0.8 μL/mL and 0.1 μL/mL NEL in the air at contact and vapor conditions, respectively. The NEL also had an evident inhibitory effect on spore germination in A. flavus along with NEL concentration as well as time-dependent kinetic inhibition. The NEL presented noticeable inhibition on dry mycelium weight and synthesis of aflatoxin B1 (AFB1) by A. flavus, totally restraining AFB1 production at 0.6 μL/mL. In real food system, the efficacy of the NEL on resistance to decay development in cherry tomatoes was investigated in vivo by exposing inoculated and control fruit groups to NEL vapor at different concentration. NEL vapors at 0.1 μL/mL air concentration significantly reduced artificially contaminated A. flavus and a broad spectrum of fungal microbiota. Results obtained from presented study showed that the NEL had a great antifungal activity and could be considered as a benefit and safe tool to control food spoilage.

Inhibitory effect of nerol against Aspergillus niger on grapes through a membrane lesion mechanism

“…1) and a major component of essential oils from spices such as neroli (Citrus aurantium), rose (Rosa damascena), and lavender (Lavandula dentata, Lavandula stoechas and Lavandula multifida) (Chintaluri, Komarraju, Chintaluri, & Vemulapalli, 2015;Kumar, Sharma, Sood, & Agnihotri, 2013;Msaada et al, 2012). Its antimicrobial activity has been recently proven against some gram-positive and gram-negative bacteria, as well as the Candida albicans and Aspergillus flavus (Jirovetz et al, 2007;Tian et al, 2013), showing a high potential to improve the shelf life and safety of perishable foods (Holley & Patel, 2005). However, no reports about the effects of NEL on A. niger contamination in table grapes during storage have yet been published, and possible mechanisms of antifungal action of NEL on A. niger remain to be established.…”

Anti-Parasitic Activity of Cherry Tomato Peel Powders

“…The results also suggest the need for the following further studies that might help broaden the value of cherry tomato fruit, peels, and pomaces for ameliorating human and animal diseases. These include (a) clinical trials on the effectiveness of cherry tomato peels with the highest content of biologically active compounds; (b) defining the effectiveness of the peel powders against metronidazole-resistant trichomonads; (c) determining whether animal feed or human food, such as breads and flatbreads [ 40 ], and supplemented with the cherry peel powders, might act as functional feeds and foods to help protect animals and humans against trichomoniasis; (d) developing novel methods to protect cherry tomatoes on growing plants against deterioration (spoilage) induced by phytopathogenic fungi, including Alternaria alternata [ 56 ] and aflatoxin-producing Aspergillus flavus [ 57 , 58 , 59 ]; and (e) determining anti-trichomonad potencies of peels from new tomato varieties that contain bioactive compounds developed via plant genetics [ 28 , 30 ].…”

Antifungal activity of puroindoline protein from soft wheat against grain molds and its potential as a biocontrol agent

“…In another experimental group, aflatoxin was extracted with methanol, and aflatoxin content was determined using high‐performance liquid chromatography according to J Tian with minor modifications (Tian et al . 2013). Aflatoxin B1 was directly extracted from maize samples with 20 ml isoamyl acetate and filtering samples through 0·22‐µm syringe filters.…”