Investigation of the VDR gene polymorphisms association with susceptibility to colorectal cancer

Abstract

The effects of 1,25-dihydroxyvitamin D3 are mediated by binding to a specific intracellular vitamin D receptor (VDR), which has been identified in a variety of tissues. Certain polymorphisms in the VDR gene have been associated with various neoplasms. For this purpose, we studied whether VDR TaqI or FokI genotype are associated with serum 25-hydroxyvitamin D3 in 52 controls and 26 patients with colorectal cancer. Polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP), and agarose gel electrophoresis tecniques were used to detect these polymorphisms. We measured 25-hydroxyvitamin D3 serum levels by ELISA. The frequencies of the FF, Ff and ff genotypes were 73.1%, 11.5%, 15.4% in colorectal cancer patients and 38.5%, 59.6%, 1.9% in healthy controls, respectively. We observed the T allele in 50% and 58.7%, and the t allele in 50% and 41.3% of colorectal cancer patients and the control group, respectively. In patients with colorectal cancer who have TT genotype, serum 25-hydroxyvitamin D3 level was lower than those with Tt/tt genotype (p:0.016). The frequency of subjects with TTFf or TtFf genotype in colorectal cancer patients was very low compared with all other genotypes (OR = 0.112; 95%CI 0.030-0.419). These data suggest that VDR TtFf or TTFf genotypes may protect against colorectal carcinogenesis. However, further studies are necessary to confirm these findings.

Effect of interval between serum draw and follow-up period on relative risk of cancer incidence with respect to 25-hydroxyvitamin D level; implications for meta-analyses and setting vitamin D guidelines

“…52 The papers by Yin et al were the primary source; I also consulted the paper by Gandini et al and added three more colorectal cancer studies. [54][55][56] This analysis omitted papers presenting data on colon or rectal cancer without the combination, colorectal cancer. This analysis combines the data for colorectal cancer for males and females by…”

Meta-Analyses of Vitamin D Intake, 25-Hydroxyvitamin D Status, Vitamin D Receptor Polymorphisms, and Colorectal Cancer Risk

“…However, for ff and CRC, the only study including a higher proportion of women than men (52) showed a statistically significant positive association (RR ¼ 1.84, 1.15-2.94), whereas studies including a higher proportion of men than women (19,50,53) or an equal proportion of men/women (21,51,(54)(55)(56)(57) (Table I). From these, 4 studies also reported on BsmI (19,20,51,54) and 4 on Fok I (19,51,54,57). High heterogeneity in the analyses on TaqI (tt vs. TT) and CRC was due to 1 study (57) with very small number of cases (n ¼ 26).…”

Vitamin D receptor gene polymorphisms in type 1 diabetes mellitus: a new pattern from Khorasan province, Islamic Republic of Iran

“…A polymorphic FokI site in exon-2 results in an alternative transcription initiation site, leading to a protein variant with 3 additional amino acids at the amino terminus [4]. Polymorphic BsmI and ApaI sites are present in intron-8, and a silent T to C substitution creates a TaqI polymorphic site in exon-9 [16].…”