Investigation of the uterine structural changes in the experimental model with polycystic ovary syndrome and effects of vitamin D treatment: An ultrastructural and immunohistochemical study

Kuyucu, Yurdun; Çelik, Latife Seyran; Kendirlinan, Özge; Tap, Özgül; Mete, Ufuk

doi:10.1016/j.repbio.2018.01.002

Cited by 30 publications

(20 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This was reflected in myocardial sections of PCOS group, in this study, as significant upregulation of caspase-3 immunoreaction. In line with this, Kuyucu et al [49] has reported a significant increase in caspase-3 staining in uterine specimens of PCOS female rats.…”

Section: Discussionsupporting

confidence: 54%

Ameliorating effect of Brassica oleracea (broccoli) extract on the myocardial damage induced by polycystic ovary syndrome in the adult female albino rat

El-Mehi¹,

Faried²

2019

J Histol Histopathol

View full text Add to dashboard Cite

Polycystic ovary syndrome (PCOS) is one of the most common and complex hormonal disorders affecting the females in the childbearing period. It is associated with several comorbidities recognized as cardiovascular risk factors. This study investigated, for the first time to our knowledge, the myocardial structural changes in experimental PCOS rat model and the ameliorating effects of Brassica oleracea(broccoli) extract. Forty adult female rats were divided into four groups; control, broccoli extract treated, PCOS and PCOS treated with broccoli extract. PCOS was induced by a single intramuscular injection of estradiol valerate (EV) (16mg/kg). Four weeks after EV injection, broccoli extract (1g/ kg dissolved in distilled water) was given orally once daily for 4 weeks. At the end of the experimental period, blood samples were collected. The myocardial specimens were processed for biochemical, histological and immunohistochemical studies. PCOS group showed oxidative stress and dyslipidemia. The myocardium exhibited many degenerative changes with significant increase in the cross-sectional area of cardiac myofibers and collagen deposition.Significant upregulation of androgen receptor, caspase-3, and glucose transporter 1 (GLUT1) with downregulation of desmin and heat shock protein 70 (Hsp70) immunoreaction was also noted. Broccoli extract exerted cardioprotective effects in PCOS through antioxidant and antiandrogen activities. The results of this work may throw more light on the myocardial damage associated with PCOS and provide a new insight into the possible use of broccoli extract to alleviate these effects.

show abstract

Section: Discussionsupporting

confidence: 54%

Ameliorating effect of Brassica oleracea (broccoli) extract on the myocardial damage induced by polycystic ovary syndrome in the adult female albino rat

El-Mehi¹,

Faried²

2019

J Histol Histopathol

View full text Add to dashboard Cite

show abstract

“…Then the slides were rinsed once for 5 min in PBS/Triton. The tissue sections were covered with rabbit polyclonal anti-caspase-3 (ab4051; Abcam, UK) primary antibody (dilution factor = 1 : 500) and then incubated overnight at 4°C in a humidified chamber [22]. After rinsing the tissue sections with PBS/Triton (4 × 5 min), biotinylated secondary antibody (Mouse and rabbit specific HRP/DAB (ABC) detection IHC kit, Abcam, ab64264, UK) was applied for 10 min at a room temperature.…”

Section: Anti-caspase-3 Immunohistochemical Analysismentioning

confidence: 99%

Effects of melatonin on ovarian reserve in cigarette smoking: an experimental study

Köle

Özkan

Eraldemır

et al. 2020

aoms

View full text Add to dashboard Cite

The aim of the study was to investigate whether melatonin has a protective effect against diminished ovarian reserve induced by smoking. Material and methods: Seventy-two female Wistar-Albino rats were divided into 6 groups: group I (room air), group II (chronic cigarette smoking), group III (room air + 10 mg/kg subcutaneous melatonin), group IV (room air + 20 mg/ kg subcutaneous melatonin), group V (chronic cigarette smoking + 10 mg/kg subcutaneous melatonin), group VI (chronic cigarette smoking + 20 mg/kg subcutaneous melatonin). For 45 days, rats were exposed to cigarette smoke through a smoking machine, then subcutaneous melatonin was administered. Apoptotic index, immunohistochemical scoring, ovarian follicle counting, ovarian tissue and serum malondialdehyde (MDA), superoxide dismutase (SOD), glutathione peroxidase (GPx), and catalase (CAT) analyses were carried out. Results: All of the primordial, primary, secondary and mature follicle numbers were found to be significantly lowered in study groups. Increased HSCORE with anti-caspase-3 staining and a high follicular apoptotic index were demonstrated in the smoking group. Serum and ovarian tissue levels of MDA were found to be elevated with smoke exposure whereas lower MDA levels were determined in melatonin treated groups. Serum and tissue levels of SOD, GPx and CAT were shown to be reduced in the smoking group in comparison with melatonin treated and control groups. 20 mg/kg melatonin administration in the smoking group revealed significantly decreased HSCOREs and apoptotic indices. Conclusions: Cigarette smoking has been definitely shown to be associated with impaired ovarian reserve with respect to significantly diminished numbers of primordial, primary, secondary and mature follicles. Dose-related treatment of melatonin in smokers may provide an evidently reduced apoptotic index and improved antioxidant activity in tissue.

show abstract

“…Polycystic ovarian syndrome (PCOS) is a multisystem disease, with incidence of 6%–10% females of reproductive age worldwide. It is often accompanied by changes in metabolic functions of the body including diabetes, obesity, and tissue resistance to insulin 1 . PCOS consists of high androgen levels, lack of ovulation, and an irregular menstrual cycle, all of which are known to occur due to alterations in the hypothalamic pituitary axis and steroid synthesis in gonads and adrenal glands, where excess androgens are known to be responsible for the metabolic manifestations 2 .…”

Section: Introductionmentioning

confidence: 99%

Morphological changes in the experimental model of polycystic ovary syndrome and effects of vitamin D treatment

Azhar

Haider

Naseem

et al. 2021

J of Obstet and Gynaecol

View full text Add to dashboard Cite

Aim This research was designed to develop an animal model by inducing polycystic ovary syndrome (PCOS) with dehydroepiandrosterone (DHEA) and observe reproductive and morphological changes after treatment with vitamin D. Materials and Methods Thirty pre‐pubertal female Sprague–Dawley (SD) dams were recruited. The animals were distributed 10 each in control, PCOS and vitamin D‐treated groups. In control group 0.2 ml of sesame oil was given. PCOS group was administered DHEA by the daily dose of 6 mg/kg for 30 days. In vitamin D‐treated group, animals were injected 6 mg/kg/day DHEA daily and 120 ng 1, 25(OH) 2D3/100 g subcutaneously once a week. The occurrence of reproductive phenotypic PCOS was evaluated by estrous cycle, morphology and histological changes of ovary, uterus on light microscope. Results The results of this study showed significant weight gain, obesity, and estrous irregularity in PCOs group as compared to control and vitamin D‐treated group. Conclusion Administration of vitamin D (120 ng 1, 25(OH) 2D3/100) improved the cycle characteristics, reduced body weight and morphological features in PCOS induced animals. The results support the effect of vitamin D treatment for metabolic and reproductive characteristic features in PCOS females.

show abstract

Investigation of the uterine structural changes in the experimental model with polycystic ovary syndrome and effects of vitamin D treatment: An ultrastructural and immunohistochemical study

Cited by 30 publications

References 40 publications

Ameliorating effect of Brassica oleracea (broccoli) extract on the myocardial damage induced by polycystic ovary syndrome in the adult female albino rat

Ameliorating effect of Brassica oleracea (broccoli) extract on the myocardial damage induced by polycystic ovary syndrome in the adult female albino rat

Effects of melatonin on ovarian reserve in cigarette smoking: an experimental study

Morphological changes in the experimental model of polycystic ovary syndrome and effects of vitamin D treatment

Contact Info

Product

Resources

About