Investigation of the cytotoxicity of eukaryotic and prokaryotic antimicrobial peptides in intestinal epithelial cells in vitro

Maher, Sam; McClean, Siobhán

doi:10.1016/j.bcp.2006.01.012

Cited by 221 publications

(172 citation statements)

References 49 publications

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“…National Clinical Laboratory standards), it is paramount that such an equally rapid cytotoxicity screen can be employed to detect selective toxicity for bacterial over human cells Cytotoxicity of melittin is well established in a range of mammalian cells, notably erythrocytes [12]. Its toxicity has also been detected in hepatocytes [16], enterocytes [13], intestinal goblet cells [13], and astrocytomas [29]. Incubation of Caco-2 cells for 24…”

Section: Discussionmentioning

confidence: 99%

“…For example, structure activity studies on the AMP, melittin, have generated large numbers of structural analogues [11]. Melittin is an amphipathic cationic linear 26-mer α-peptide that has cell penetrating activity as well as well-known cytotoxicity in erythrocytes [12] and enterocytes [13] and other mammalian cells [14]. Alongside phospholipase A2, it is a major constituent of the venom of Apis Mellifera, and has been found to cause apoptosis and necrosis in different cells [15,16], as well as agonist and antagonist actions on many cellular proteins including phospholipase A2 and ATPases (reviewed in [14]).…”

Section: Introductionmentioning

confidence: 99%

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High content analysis to determine cytotoxicity of the antimicrobial peptide, melittin and selected structural analogs

et al. 2011

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Antimicrobial peptides (AMPs) are naturally occurring entities with potential as pharmaceutical candidates and/or food additives. They are present in many organisms including bacteria, insects, fish and mammals. While their antimicrobial activity is equipotent with many commercial antibiotics, current limitations are poor pharmacokinetics, stability and potential toxicology issues. Most elicit antimicrobial action via perturbation of bacterial membranes. Consequently, associated cytotoxicity in human cells is reflected by their capacity to lyse erythrocytes. However, more rigorous toxicological assessment of AMPs is required in order to predict potential failure at a later stage of development. We describe a high-content analysis (HCA) screening protocol recently established for determination and prediction of safety in pharmaceutical drug discovery. HCA is a powerful, multi-parameter bioanalytical tool that amalgamates the actions of fluorescence microscopy with automated cell analysis software in order to understand multiple changes in cellular health. We describe the application of HCA in assessing cytotoxicity of the cytolytic α-helical peptide, melittin, and selected structural analogues. The data shows that structural modification of melittin reduces its cytotoxic action and that HCA is suitable for rapidly identifying cytotoxicity.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

High content analysis to determine cytotoxicity of the antimicrobial peptide, melittin and selected structural analogs

et al. 2011

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“…The number of dead cells was determined by an MTT cell proliferation assay after 24 h of incubation with the complexes. [15] This method is a colorimetric assay system which measures the reduction of a tetrazolium component (MTT) to an insoluble formazan product by the mitochondria of viable cells. The amount of color produced is directly proportional to the number of viable cells.…”

Section: Cytotoxic Effectmentioning

confidence: 99%

Synthesis, Characterization and Antibacterial Activity of Cobalt(iii) Complex With Phenanthroline and Maltose

Parada

Atria

Wiese

et al. 2014

J. Chil. Chem. Soc.

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The mononuclear cobalt(III) complex derived from 1,10-phenanthroline and maltose ([Co(phen) 2 maltose]Cl 2 •3H 2 O) (1) has been synthesized and characterized in aqueous solution. Its characterization was based on its optical and spectroscopic properties.The antimicrobial activity of this complex was screened in vitro against the microorganisms Escherichia coli DH5a, Salmonella enterica sv Enteritidis ISP/953, Klebsiella pneumoniae RYC492, Pseudomonas aeruginosa PAO1, Enterococcus faecalis ATCC 29212, Bacillus cereus GCA234, Micrococcus sp. Staphylococcus aureus ATCC25923.Complex (1) showed antibacterial activity with a bacteriostatic effect over Gram positive and negative bacteria. The cytotoxicity of complex (1) was tested in vitro on human embryonic kidney cells.Keywords: Carbohydrate-cobalt(III) complexes; circular dichroism; antibacterial activity.e-mail: jparada@ciq.uchile.cl INTRODUCTIONTransition metal complexes are of interest since they display a wide variety of application in fields ranging from materials science and catalysis to biological activity [1].From the biological point of view, several studies have shown that the complexes may have antibacterial, antifungal and antitumor activity [2].The biological activity of metal complexes is highly dependent on the nature of the metal ions and the donor sequence of the ligands because different ligands exhibit different biological properties [3].An interesting group of ligands are the carbohydrates [4], whose complexes have shown significant antimicrobial activity against Gram-positive and Gramnegative bacterial strains as well as a few fungal strains [5]. Poller and Parkin reported the synthesis of organometallic derivatives of sucrose (lead, tin and germanium). In that study organotin was found to exhibit higher biocidal activities than would be expected from the tin content [6].A few sugar-cobalt complexes with antibacterial activity have been reported. An important example is grafted PVA polymer with a derivative of erythro-ascorbic acid (pentulosono-lactone-2,3-enedianisoate) reported by Salih et al. It was evaluated for antimicrobial properties against four pathogenic bacteria (Escherichia coli, Klebsiella pneumonae, Staphylococcus aureus, Staphylococcus albus) and fungi (Aspergillus niger, yeast). This cobalt complex showed good activity against the various microbial isolates [7].1,10-Phenanthroline is also one of the biologically important ligands, and several studies show that this ligand and a number of its complexes are effective against various strains of microorganisms. Here the synthesis of the mixed ligand cobalt complex [Co(phen) 2 maltose] Cl 2 ·3H 2 O (1), as well as its spectral features and optical activity are reported.UV-visible absorption spectroscopy and circular dichroism spectroscopy allow us to assign a D configuration to complex (1).The complex was also screened for antibacterial activity against Gram positive and Gram-negative bacteria. We determined the antibacterial effects and evaluated their cytotoxic effect on h...

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“…The number of living cells was determined using methyl thiazolyldiphenyl-tetrazolium bromide (MTT) assay (Sigma, Germany) based on the reduction of the tetrazolium salt, methyl thiazolyldiphenyl-tetrazolium bromide into a crystalline blue formazan product by the cellular oxidoreductases of viable cells (Maher and McClean, 2006). The resultant formazan crystal formation is proportional to the number of living cells.…”

Section: Cell Viabilitymentioning

confidence: 99%

Synthesis and characterization of cobalt ferrites nanoparticles with cytotoxic and antimicrobial properties

Hathout¹,

Aljawish²,

Sabry³

et al. 2017

J App Pharm Sci

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Recently, the application of nanotechnology in food sector and the agriculture attract the attention compared to its biomedical application. The aims of the current study was to synthesize and characterize cobalt ferrites nanoparticles [(CoFe2O4) NPs] by combustion method employing glycine as fuels and to evaluate their antimicrobial against pathogenic bacteria and fungi and anti cancer properties against MCF-7 breast cancer cells line. The results indicated that the particles size of the synthesized (CoFe2O4) NPs was 40 nm. These (CoFe2O4) NPs showed potential antibacterial properties against Gram-negative bacteria (Escherichia coli, Salmonella typhi) and Gram-positive bacteria (Staphylococcus aureus, Bacillus cereus) as well as the pathogenic fungi (Aspergillus flavus and Aspergillus ochraceus) in a dose dependent manner with maximum concentration of 1.8 mg/ml. (CoFe2O4) NPs also showed weak antiradical but have cytotoxic effects against MCF-7 breast cancer cells line and succeeded to decrease the cell viability at a concentration of 2 mg/ml. It could be concluded that (CoFe2O4) NPs is a promise candidate as antimicrobial and anticancer agent for food sector and medical application.

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Investigation of the cytotoxicity of eukaryotic and prokaryotic antimicrobial peptides in intestinal epithelial cells in vitro

Cited by 221 publications

References 49 publications

High content analysis to determine cytotoxicity of the antimicrobial peptide, melittin and selected structural analogs

High content analysis to determine cytotoxicity of the antimicrobial peptide, melittin and selected structural analogs

Synthesis, Characterization and Antibacterial Activity of Cobalt(iii) Complex With Phenanthroline and Maltose

Synthesis and characterization of cobalt ferrites nanoparticles with cytotoxic and antimicrobial properties

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