Investigation of taxa of the family Pasteurellaceae isolated from Syrian and European hamsters and proposal of Mesocricetibacter intestinalis gen. nov., sp. nov. and Cricetibacter osteomyelitidis gen. nov., sp. nov.

Abstract

Eleven strains from hamster of Bisgaard taxa 23 and 24, also referred to as Krause’s groups 2 and 1, respectively, were investigated by a polyphasic approach including data published previously. Strains showed small, regular and circular colonies with smooth and shiny appearance, typical of members of the family Pasteurellaceae . The strains formed two monophyletic groups based on 16S rRNA gene sequence comparison to other members of the family Pasteurellaceae . Partial rpoB sequencing as well as published data on DNA–DNA hybridization showed high genotypic relationships within both groups. Menaquinone 7 (MK7) was found in strains of both groups as well as an unknown ubiquinone with shorter chain length than previously reported for any other member of the family Pasteurellaceae . A new genus with one species, Mesocricetibacter intestinalis gen. nov., sp. nov., is proposed to accommodate members of taxon 24 of Bisgaard whereas members of taxon 23 of Bisgaard are proposed to represent Cricetibacter osteomyelitidis gen. nov., sp. nov. Major fatty acids of type strains of type species of both genera are C14 : 0, C14 : 0 3-OH/iso-C16 : 1 I, C16 : 1ω7c and C16 : 0. The two genera are clearly separated by phenotype from each other and from existing genera of the family Pasteurellaceae . The type strain of Mesocricetibacter intestinalis is HIM 933/7T ( = Kunstyr 246/85T = CCUG 28030T = DSM 28403T) while the type strain of Cricetibacter osteomyelitidis is HIM943/7T ( = Kunstyr 507/85T = CCUG 36451T = DSM 28404T).

Testudinibacter aquarius gen. nov., sp. nov., a member of the family Pasteurellaceae isolated from the oral cavity of freshwater turtles

“…Mesocricetibacter and Cricetibacter express only menaquinone-7 (Christensen et al, 2014b), whereas all previously investigated Pasteurellaceae express between two and five different quinones (Hansen et al, 2012). A comparison with other members is given in Table S3.…”

Reclassification of Actinobacillus muris as Muribacter muris gen. nov., comb. nov.

“…AY078999 for Pasteurella multocida NCTC 10322 T ) compared with other members of Pasteurellaceae (Christensen et al, 2011). Mesocricetibacter intestinalis and Cricetibacter osteomyelitidis were recently described based on the characterization of strains isolated from hamsters (Christensen et al, 2014). In the present study, a collection of strains belonging to the Pasteurellaceae obtained from rodents was subjected to extended phenotypic characterization.…”

“…In addition, mice and rats were bought from 10 different pet shops. Isolates were subjected to phenotypic characterization using 40 biochemical criteria examined by conventional tests as reported previously (Christensen et al, 2014). In these tests, acid formation from carbohydrates was tested in phenol red broth base (Difco Laboratories) supplemented with 1 % of the respective carbohydrate and read after 2-3 days of incubation at 37 8C.…”

“…Two sequences were excluded from the 16S rRNA Table 1. Phenotypic separation of Muribacter gen. nov. from existing genera of the Pasteurellaceae Genera: 1, Muribacter gen. nov.; 2, Mesocricetibacter (data from Christensen et al, 2014); 3, Cricetibacter (Christensen et al, 2014); 4, Haemophilus sensu stricto Nørskov-Lauritsen et al, 2005;Winslow et al, 1917;Zinnemann & Biberstein, 1974); 5, Actinobacillus sensu stricto (Brumpt, 1910;Christensen & Bisgaard, 2004); 6, Lonepinella (Osawa et al, 1995); 7, Mannheimia (Angen et al, 1999); 8, Pasteurella sensu stricto (Trevisan, 1887;Mutters et al, 1985;Christensen & Bisgaard, 2006); 9, Phocoenobacter (Foster et al, 2000); 10, Gallibacterium (Bisgaard et al, 2009); 11, Volucribacter (Christensen et al, 2004a); 12, Histophilus (Angen et al, 2003); 13, Avibacterium (Blackall et al, 2005); 14, Nicoletella (Kuhnert et al, 2004); 15, Bibersteinia (Blackall et al, 2007); 16, Aggregatibacter (Nørskov-Lauritsen & Kilian, 2006); 17, Basfia (Kuhnert et al, 2010); 18, Chelonobacter (Gregersen et al, 2009); 19, Necropsobacter (Christensen et al, 2011); 20, Bisgaardia (Foster et al, 2011); 21, Otariodibacter (Hansen et al, 2012); 22, Frederiksenia (Bisgaard & Mutters, 1986;Korczak et al, 2014);23, Vespertiliibacter (Mühldorfer et al, 2014). Characters are scored as: +, 90 % or more of strains positive within 1-2 days; 2, less than 10 % of strains positive within 14 days; (+), 90 % or more of the strains positive within 3-14 days; d, 11-89 % of strains positive; W, weakly positive; ND, no data available.…”

Ursidibacter maritimus gen. nov., sp. nov. and Ursidibacter arcticus sp. nov., two new members of the family Pasteurellaceae isolated from the oral cavity of bears

“…Table 1. Phenotypic characteristics that distinguish Ursidibacter gen. nov. from other genera of the family Pasteurellaceae Genera: 1, Ursidibacter gen. nov. (data from this study); 2 Haemophilus sensu stricto (Nørskov-Lauritsen et al, 2005); 3, Actinobacillus sensu stricto ; 4, Lonepinella (Osawa et al, 1995); 5, Mannheimia (Angen et al, 1999); 6, Pasteurella sensu stricto (Christensen & Bisgaard, 2006); 7, Phocoenobacter (Foster et al, 2000); 8, Gallibacterium (Bisgaard et al, 2009); 9, Volucribacter ; 10, Histophilus (Angen et al, 2003a); 11, Avibacterium (Blackall et al, 2005); 12, Nicoletella (Kuhnert et al, 2004); 13, Bibersteinia (Blackall et al, 2007);14, Aggregatibacter (Nørskov-Lauritsen & Kilian, 2006;Patel et al, 2004); 15, Basfia (Kuhnert et al, 2010); 16, Chelonobacter (Gregersen et al, 2009); 17, Necropsobacter (Christensen et al, 2011b); 18, Bisgaardia (Foster et al, 2011;Hansen et al, 2015);19, Otariodibacter (Hansen et al, 2012); 20, Frederiksenia (Korczak et al, 2014); 21, Mesocricetibacter (Christensen et al, 2014b); 22, Cricetibacter (Christensen et al, 2014b);23, Vespertiliibacter (Mühldorfer et al, 2014). +, Only positive reactions occur; 2, only negative reactions occur; d, positive or negative; ND, no data available.…”