Reclassification of Actinobacillus muris as Muribacter muris gen. nov., comb. nov.

Abstract

Reclassification of Actinobacillus muris as Muribacter muris gen. nov., comb. nov. To reinvestigate the taxonomy of [Actinobacillus ] muris, 474 strains, mainly from mice and rats, were characterized by phenotype and 130 strains selected for genotypic characterization by 16S rRNA and partial rpoB gene sequencing. The type strain was further investigated by wholegenome sequencing. Phylogenetic analysis of the DNA sequences showed one monophyletic group with intragroup similarities of 96.7 and 97.2 % for the 16S rRNA and rpoB genes, respectively. The highest 16S rRNA gene sequence similarity to a taxon with a validly published name outside the group was 95.9 %, to the type strain of [Pasteurella] pneumotropica. The closest related taxon based on rpoB sequence comparison was 'Haemophilus influenzaemurium', with 88.4 % similarity. A new genus and a new combination, Muribacter muris gen. nov., comb. nov., are proposed based on a distinct phylogenetic position based on 16S rRNA and rpoB gene sequence comparisons, with major divergence from the existing genera of the family Pasteurellaceae. The new genus has the characteristics of [A.] muris with the emendation that acid formation from (2)-D-mannitol and hydrolysis of aesculin are variable, while the a-glucosidase test is positive. There is no requirement for exogenously supplied NAD (V factor) for the majority of strains investigated; however, one strain was found to require NAD. The major fatty acids of the type strain of Muribacter muris were C 14 : 0 , C 14 : 0 3-OH/iso-C 16 : 1 I, C 16 : 1 v7c and C 16 : 0 , which is in line with most genera of the Pasteurellaceae. The type strain of Muribacter muris is CCUG 16938 T (5NCTC 12432 T 5ATCC 49577 T ).

Early‐life malnutrition causes gastrointestinal dysmotility that is sexually dimorphic

“…This increase in Proteobacteria was driven by expansion of a single species, Muribacter muris ( Figure 8F), a rodent commensal bacterium in the family Pasteurellaceae. 38 These results indicate that malnutrition-induced changes in GI motility, morphology, contractility, and permeability are associated with altered gut microbial communities and are dependent on host sex.…”

Nasal Delivery of a Commensal Pasteurellaceae Species Inhibits Nontypeable Haemophilus influenzae Colonization and Delays Onset of Otitis Media in Mice

“…H. haemolyticus does not colonize mice (our unpublished data); therefore, we sought alternatives to further investigate microbial interference of NTHi in vivo. In this study, we have used Muribacter muris, a closely related rodent equivalent of H. haemolyticus from the Pasteurellaceae family (25), in a murine model of NTHi acute otitis media. We have assessed whether intranasal pretreatment of mice with M. muris can be used to prevent NTHi colonization and development of disease.…”

Identification ofLonepinellasp. in Koala Bite Wound Infections, Queensland, Australia

“…All isolates of this study had an identity of >95% for the rpoB gene of ACM 3666 (Table 2), indicating a close genetic relationship with L. koalarum . A minimum level of 83%–85% identity of the partial infB gene has been shown between members of the Pasteurellaceae family at the genus level ( 12 ). The partial infB gene sequence of MS14434 had a high similarity (99.78%) to the corresponding sequence in ACM 3666, and the partial infB genes of the other 3 isolates are at the lower limit of the 83%–85% threshold.…”