Ursidibacter maritimus gen. nov., sp. nov. and Ursidibacter arcticus sp. nov., two new members of the family Pasteurellaceae isolated from the oral cavity of bears

Hansen, Mie Johanne; Braaten, Mira Strøm; Bojesen, Anders Miki; Sonne, Christian; Dietz, Runé; Bertelsen, Mads F.

doi:10.1099/ijsem.0.000476

Cited by 18 publications

(2 citation statements)

References 47 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The PM shared 60% sequence similarity with G. parasuis in epitope 5D11, having six different amino acids at P 325 , Q 326 , E 328 , N 330 , L 331 , and A 336 . Among them, Nicoletella and Mannheimia mainly infected horses [20], cattle [21] and sheep [22], and Ursidibacter from the bear mouth [23]. However, Actinobacillus indolicus [29], APP [30], and PM [31] can infect pigs.…”

Section: Discussionmentioning

confidence: 99%

“…Meanwhile, the results revealed that Nicoletella semolina, Mannheimia haemolytica, Mannheimia pernigra, Ursidibacter maritimus, Ursidibacter arcticus, and Actinobacillus indolicus, share high sequence similarity in the position of epitope 5D11. Among them, Nicoletella and Mannheimia mainly infected horses [20], cattle [21] and sheep [22], and Ursidibacter from the bear mouth [23]. The APP shares 80% sequence similarity with G. parasuis in epitope 5D11, having three different amino acids at P 325 , N 330 , and A 336 .…”

Section: Alignment Analysismentioning

confidence: 99%

See 1 more Smart Citation

Identification of a Novel Linear B-Cell Epitope of HbpA Protein from Glaesserella parasuis Using Monoclonal Antibody

Liu

Wang

Yang

et al. 2023

IJMS

View full text Add to dashboard Cite

Glaesserella parasuis (G. parasuis.) is the etiological pathogen of Glässer’s disease, which causes high economic losses to the pig industry. The heme-binding protein A precursor (HbpA) was a putative virulence-associated factor proposed to be potential subunit vaccine candidate in G. parasuis. In this study, three monoclonal antibodies (mAb) 5D11, 2H81, and 4F2 against recombinant HbpA (rHbpA) of G. parasuis SH0165 (serotype 5) were generated by fusing SP2/0-Ag14 murine myeloma cells and spleen cells from BALB/c mice immunized with the rHbpA. Indirect enzyme-linked immunosorbent assay (ELISA) and indirect immunofluorescence assay (IFA) demonstrated that the antibody designated 5D11 showed a strong binding affinity with the HbpA protein and was chosen for subsequent experiments. The subtypes of the 5D11 were IgG1/κ chains. Western blot analysis showed that mAb 5D11 could react with all 15 serotype reference strains of G. parasuis. None of the other bacteria tested reacted with 5D11. In addition, a linear B-cell epitope recognized by 5D11 was identified by serial truncations of HbpA protein and then a series of truncated peptides were synthesized to define the minimal region that was required for mAb 5D11 binding. The 5D11 epitope was located on amino acids 324-LPQYEFNLEKAKALLA-339 by testing the 5D11 monoclonal for reactivity with 14 truncations. The minimal epitope 325-PQYEFNLEKAKALLA-339 (designated EP-5D11) was pinpointed by testing the mAb 5D11 for reactivity with a series of synthetic peptides of this region. The epitope was highly conserved among G. parasuis strains, confirmed by alignment analysis. These results indicated that mAb 5D11 and EP-5D11 might potentially be used to develop serological diagnostic tools for G. parasuis. Three-dimensional structural analysis revealed that amino acids of EP-5D11 were in close proximity and may be exposed on the surface of the HbpA protein.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Alignment Analysismentioning

confidence: 99%

Identification of a Novel Linear B-Cell Epitope of HbpA Protein from Glaesserella parasuis Using Monoclonal Antibody

Liu

Wang

Yang

et al. 2023

IJMS

View full text Add to dashboard Cite

show abstract

Pasteurellaceae

Kuhnert¹,

Busse²,

Blackall³

et al. 2020

Bergey's Manual of Systematics of Archaea and Bacteria

View full text Add to dashboard Cite

Pas.teu.rel.la.ce'ae. N.L. fem. n. Pasteurella type genus of the family; ‐aceae suffix to denote a family; N.L. fem. pl. n. Pasteurellaceae the Pasteurella family. Proteobacteria / Gammaproteobacteria / Pasteurellales / Pasteurellaceae Members of the family Pasteurellaceae are Gram‐negative and nonmotile. They are negative for growth on Simmon's citrate agar, and negative tests are observed with mucate acid, malonate base, and growth in KCN. Nitrate is reduced without gas formation, and they are positive in alanine aminopeptidase. (+)‐ D ‐Glucose is metabolized or fermented in the Hugh and Leifson test with the exception of Nicoletella . Members of Pasteurellaceae are negative in arginine dihydrolase and phenylalanine deaminase tests, and tests for hydrolysis of gelatin, Tween, and α‐mannosidase are also negative, and they do not produce acid from meso ‐erythritol, adonitol, (−)‐ L ‐xylose, (+)‐ D ‐fucose, (−)‐ L ‐sorbose, (+)‐ D ‐melezitose, (+)‐ D ‐turanose, and β‐ N ‐CH 3 ‐glucosamine. The family forms a monophyletic group based on 16S rRNA gene and conserved protein sequence comparisons. The genera Aggregatibacter, Avibacterium, Basfia, Bibersteinia, Bisgaardia, Caviibacterium, Chelonobacter, Conservatibacter, Cricetibacter, Frederiksenia, Gallibacterium, Glaesserella, Histophilus, Lonepinella, Mannheimia, Mesocricetibacter, Muribacter, Necropsobacter, Nicoletella, Otariodibacter, Phocoenobacter, Rodentibacter, Seminibacterium, Testudinibacter, Ursidibacter, Vespertiliibacter , and Volucribacter can be regarded as monophyletic or monotypic, whereas some species of the genera Actinobacillus , Haemophilus , and Pasteurella are still polyphyletic with their type species. The major polar lipid profile consists of phosphatidylethanolamine and phosphatidylglycerol. The dominant fatty acids are C 14:0 , C 16:0 , C 16:1 ω7 c , and C 14:0 3‐OH/C 16:1 ISO‐I. Demethylmenaquinones, menaquinones, and ubiquinones with chain lengths of seven or/and eight may be produced. Members of the family have been found with the polyamines putrescine, diaminopropane, cadaverine, spermidine, tyramine, spermine, sym‐norspermidine, and sym‐ homospermidine. Members of Pasteurellaceae are associated with animals and human beings as commensals, opportunistic pathogens, or as primary pathogens. Some have clinical importance, and the distribution seems global. The energy metabolism is chemoheterotrophic, and they are facultative anaerobic, microaerophilic, or aerobic. Type genus : Pasteurella Trevisan 1887 AL (nom. cons. Opinion 13 1954) emend. Mutters et al. 1985b VP . DNA G + C content (mol%) : 36–49.

show abstract

Ursidibacter

Hansen¹

2020

Bergey's Manual of Systematics of Archaea and Bacteria

View full text Add to dashboard Cite

Ur.si.di.bac'ter. N.L. fem. pl. n. Ursidae , taxonomic name for the bear family; N.L. masc. n. bacter a rod; N.L. masc. n. Ursidibacter rod isolated from Ursidae . Proteobacteria / Gammaproteobacteria / Pasteurellales / Pasteurellaceae / Ursidibacter Nonmotile, nonhemolytic, fermentative, and Gram‐negative rods that are catalase‐ and oxidase‐positive. Positive reactions are obtained for ONPG and PNPG. Negative reactions are obtained for methyl red, alkaline phosphatase, and urease. Do not require X‐ or V‐factors. Nitrates are reduced to nitrites, but nitrites are not reduced. Acid is produced from l ‐arabinose, dulcitol, d ‐mannitol, d ‐mannose, maltose, l ‐rhamnose, and trehalose but not from d ‐sucrose. Variable reactions are obtained for indole. The main fatty acids for the type strain Pb43106 are C 14:0 , C 16:1 ω7 c , C 16:0 , and summed features C 18:2 ω6,9 c /C 18:0 ANTE. Respiratory quinones are ubiquinone‐7 (55%) and ubiquinone‐8 (45%) and the DNA G + C content 37.9 mol%. Ursidibacter has been isolated from the oral cavity of healthy polar and brown bears. DNA G + C content (mol%) : 37.9 to 38.03 (genome analysis). Type species : Ursidibacter maritimus Hansen et al. 2015 VP .

show abstract

Ursidibacter maritimus gen. nov., sp. nov. and Ursidibacter arcticus sp. nov., two new members of the family Pasteurellaceae isolated from the oral cavity of bears

Cited by 18 publications

References 47 publications

Identification of a Novel Linear B-Cell Epitope of HbpA Protein from Glaesserella parasuis Using Monoclonal Antibody

Identification of a Novel Linear B-Cell Epitope of HbpA Protein from Glaesserella parasuis Using Monoclonal Antibody

Pasteurellaceae

Ursidibacter

Contact Info

Product

Resources

About