Investigation of synergistic potential of green tea polyphenols and orlistat combinations using pancreatic lipase assay-based synergy directed fractionation strategy

George, Ginson; ̇, Sridhar S ̇N ̇C; Paul, Atish T.

doi:10.1016/j.sajb.2020.08.009

Cited by 12 publications

(4 citation statements)

References 23 publications

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“…Hence, it is essential to understand its effect on the enzyme activity in advance, especially when subjecting the enzyme to consecutive reactions. Literature reports on PPL suggest using less than 5% (v/v) of DMSO in enzymatic reactions because higher concentrations affect enzyme performance [37][38][39][40].…”

Section: Operational Stabilitymentioning

confidence: 99%

3D Printed Polylactic Acid (PLA) Well Plates for Enzyme Inhibition Studies: The Case of Pancreatic Lipase

STAMATIS¹,

Gournis²,

POLYDERA³

et al. 2022

Catal Res

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3D printed PLA has already been demonstrated for several biotechnological applications, including enzymes immobilization. The prerequisites for an efficient screening assay include using small volumes of reagents, low cost, and rapid screening of large numbers of compounds and extracts. Hence, assays based on microtiter plates are predominant. Thus, designing and fabricating scaffolds on a similar scale, which could serve as immobilization carriers, and their recruitment in inhibitors screening studies is of great significance, adding both enzyme stability and reuse potentiality of the biocatalytic system in assay merits. In this work, pancreatic lipase was immobilized on 3D-printed PLA microwells for enzyme inhibitor screening. XPS analysis demonstrated the successful modification of the PLA scaffolds. The immobilized enzyme displayed high levels of operational, thermal, and storage stability under the tested conditions. The IC<sub>50</sub> values for PPL inhibition were calculated for Orlistat, a model lipase inhibitor, and olive leaf extract, a promising natural compound. This is the first study reporting the use of 3D-printed PLA wells with an immobilized enzyme for inhibitor screening assay.

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Section: Operational Stabilitymentioning

confidence: 99%

3D Printed Polylactic Acid (PLA) Well Plates for Enzyme Inhibition Studies: The Case of Pancreatic Lipase

STAMATIS¹,

Gournis²,

POLYDERA³

et al. 2022

Catal Res

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“…According to an experimental report, TPs not only have a significant therapeutic effect when used alone, but also has a strong auxiliary effect when combined with other drugs. For example, the synergistic effect of anti-obesity can be produced by the combination of TPs and orlistat (Ginson et al, 2020).…”

Section: Lowering Lipid and Liver Protectionmentioning

confidence: 99%

Molecular mechanisms and applications of tea polyphenols: A narrative review

et al. 2021

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Tea not only has a beloved taste, but also has rich biological activity, so it has become one of the best-selling beverages in the world. In the existing studies on tea, it is found that TPs account for highest proportion, which is the main bioactive ingredient. Generally, green tea polyphenols (GTP S ) are the main research materials, which are high in catechins. GTP S have significant health benefits (Xing et al., 2019) and are considered to be safe by the US Food and Drug Administration (Chang et al., 2019). Since the 1950s, scholars have begun to study the biological activity of TPs and their mechanism of action, and found that TPs are not only beneficial to human health, but also have an impact on food, medical, chemical and other fields.According to the research on the components of TPs (Khan & Mukhtar, 2018;Li et al., 2019), it is found that TPs contain catechins, flavonoids, anthocyanins, phenolic acids and other substances, among which catechins account for the largest proportion, up to 60%-80%. Catechin is divided into epicatechin (EC), epicatechin gallate (ECG), epigallocatechin (EGC) and epigallocatechin gallate (EGCG; the molecular structure is shown in the Figure 1). The content relationship is EGCG > EGC > ECG > EC, of which EGCG accounts for about 70%. Therefore, EGCG is the main catechin and the research on the biological activity of TPs mainly starts from EGCG.The research conclude that TPs can exert a variety of biological activities such as anti-oxidation, anti-tumor, anti-inflammation,

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“…Inhibition of food digestion and absorption aimed to reduce energy intake are significant obesity treatment strategies (Marrelli et al 2013). Pancreatic lipase (PL) secreted by the pancreas plays an important role in fat metabolism and is responsible for converting triglycerides to glycerol and fatty acids by hydrolysis (George et al 2020, Liu et al 2020. Carbohydrates are absorbed in the small intestine after being broken down into monosaccharides by α-amylase and α-glucosidase as the two major enzymes responsible for their hydrolysis.…”

Section: Introductionmentioning

confidence: 99%

α-AMYLASE, α-GLUCOSIDASE AND LIPASE INHIBITORY PROPERTIES AND PHYTOCHEMICAL ANALYSIS OF ENDEMIC PLANT Jurinea brevicaulis Boiss.

Şener

Kanbolat

Çolak

et al. 2023

Trakya University Journal of Natural Sciences

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Obesity, defined as new world syndrome, is an epidemic that causes global health problems and big economic losses. Natural products have been gain increasing importance for the epidemic because of their antiobesity potency. Jurinea genus comprised approximately 200 species worldwide and has been used as a therapeutic agent for colic, fever, gout, and rheumatism, traditionally. The aim of this study was to analyze the volatile components, to determine its phenolic compounds and to evaluate its antiobesity potentcy of Jurinea brevicaulis BOISS. A widespread method based on solid-phase microextraction technique (SPME) was employed for GC-MS analysis of volatile components of J. brevicaulis. Quantitative analysis of phenolic compounds of J. brevicaulis was performed using RP-HPLC. To evaluate the antiobesity effect, the inhibitory effects of α-amylase, α-glucosidase, and lipase of the species were determined. A total of 19 volatile components were specified and o-cymene (10.60 %), β-Bisabolene (9.30 %), and sesquicineole (57.5 %) for different terpenes were described as major components. According to HPLC analysis, sinapic acid, p-coumaric acid, and quercetin were determined for the species. IC50 value of the species was determined as 36.59 ± 2.37 μg/mL and 42.56 ± 2.83 μg/mL for α-amylase and α-glucosidase assays, respectively. IC50 value was found 50.31 ± 3.75 μg/mL, according to lipase inhibition studies. Considering all the findings, it has been determined that the species is included diverse volatile components, antiobesity effective three phenolic compounds, and showed promising antiobesity potential so J. brevicaulis may be the up-and-coming candidate of natural product for obesity.

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Investigation of synergistic potential of green tea polyphenols and orlistat combinations using pancreatic lipase assay-based synergy directed fractionation strategy

Cited by 12 publications

References 23 publications

3D Printed Polylactic Acid (PLA) Well Plates for Enzyme Inhibition Studies: The Case of Pancreatic Lipase

3D Printed Polylactic Acid (PLA) Well Plates for Enzyme Inhibition Studies: The Case of Pancreatic Lipase

Molecular mechanisms and applications of tea polyphenols: A narrative review

α-AMYLASE, α-GLUCOSIDASE AND LIPASE INHIBITORY PROPERTIES AND PHYTOCHEMICAL ANALYSIS OF ENDEMIC PLANT Jurinea brevicaulis Boiss.

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