Investigation of porcine FABP3 and LEPR gene polymorphisms and mRNA expression for variation in intramuscular fat content

Li, Xiaoping; Kim, Sang Wook; Choi, Jung‐Suk; Lee, Yoon Mi; Lee, Cheol Koo; Choi, Bong Hwan; Kim, Tae Hun; Choi, Yang-Il; Kim, Jong Joo; Kim, Kwan‐Suk

doi:10.1007/s11033-010-0050-1

Cited by 54 publications

(33 citation statements)

References 40 publications

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“…The H-FABP polymorphisms have been studied in many pig populations, with significant associations being observed between these polymorphisms and the IMF content (Gerbens et al, 1997(Gerbens et al, , 2000Arnyasi et al, 2006;Lee et al, 2010;Li et al, 2010;Han et al, 2012). These studies indicated that the ordering of IMF H-FABP genotypes is HH > Hh > hh, DD < Dd < dd, and AA < Aa < aa, and that porcine meat quality might be improved by increasing the frequency of genotype aa-dd-HH in pig breeds (Pang et al, 2006).…”

Section: Discussionmentioning

confidence: 99%

Distribution of H-FABP and ACSL4 gene polymorphisms and their associations with intramuscular fat content and backfat thickness in different pig populations

et al. 2014

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ABSTRACT. Here, we analyzed the distribution of H-FABP/ (HinfI, MspI, and HaeIII) and ACSL4/RsaI polymorphisms, and the associations of these 4 polymorphic loci with intramuscular fat (IMF) content and backfat thickness (BFT) RsaI polymorphisms were absent in Yanan and Jinhua pigs. Linkage disequilibrium analysis indicated that the 3 loci (HinfI, MspI, and HaeIII) were separated. Association analysis showed that the H-FABP/HinfI locus significantly affected IMF content in DLY (P < 0.05) and Yanan (P < 0.001) pigs. The highest IMF content was recorded in the adH haplotype of the 3 H-FABP polymorphic loci (2.59%, P < 0.05) in DLY pigs. At the ACSL4/RsaI locus, higher IMF content was recorded for sows with a GG genotype or boars with a G haplotype compared to those with an AA genotype (2.53 vs 2.10%, P < 0.05) or A haplotype (2.48 vs 1.73%, P < 0.01) in DLY pigs. Significant differences were not obtained among these 4 polymorphic loci and BFT (P > 0.05). The results indicate that H-FABP and ACSL4 genes might serve as markers to improve IMF content (but not BFT) in the pig breeding system.

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Section: Discussionmentioning

confidence: 99%

Distribution of H-FABP and ACSL4 gene polymorphisms and their associations with intramuscular fat content and backfat thickness in different pig populations

et al. 2014

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“…An increased IMF content was associated with higher juiciness and flavor scores (Fernandez et al, 1999a). Because of the positive relationship between IMF content and meat tenderness (Wood et al, 2004;Fortin et al, 2005;Chmurzyńska, 2006), numerous genes associated with fat metabolism in humans and mice have been applied to pig breeding to improve IMF content; these genes include NUDT6, FABP3, H-FABP, and FTO (Fontanesi et al, 2009;Li et al, 2010;Ding et al, 2011;Sun et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Molecular characterization and tissue expression profile analysis of the porcine JAZF1 gene

Yang¹,

He²,

Xu³

et al. 2015

Genet. Mol. Res.

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ABSTRACT.A recent study indicated that the JAZF1 gene was related to lipid metabolism by regulating the level of gene expression in humans and mice. In order to investigate whether JAZF1 gene expression was associated with fat deposition in pig, we cloned the full-coding region of the JAZF1 gene (GenBank accession No. KF307636) from porcine longissimus dorsi. Results showed that the open reading frame of JAZF1 covered 732 bp and encoded 243 amino acids. Multiple alignment of isoform sequences revealed that the deduced amino acid sequence of JAZF1 had a high degree of sequence similarity to other vertebrates, indicating that it was highly conserved during evolution. Bioinformatic analysis indicated that pig JAZF1 contained 23 phosphorylation sites and 19 glycosyl sites. JAZF1 was predicted to have 3 ZnF-C 2 H 2 and 2 low-complexity domains. The JAZF1 mRNA expression pattern indicated that JAZF1 mRNA expression level in the liver was significantly different in 2 divergent breeds (P < 0.05). This article perhaps provided an important experimental basis for further research on the mechanisms of lipid metabolism and fat deposition in pigs.

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“…The suggested optimal range of IMF content for achieving eating satisfaction and meeting dietetic requirements is 2.5-3.0% (Li et al, 2010). There was a significant influence of the L-FABP gene polymorphism on IMF in Junmu No.…”

Section: Discussionmentioning

confidence: 92%

Association of T1740C polymorphism of L-FABP with meat quality traits in Junmu No. 1 white swine

Yh¹,

Ls²,

Ma³

et al. 2013

Genet. Mol. Res.

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ABSTRACT. This study was designed to investigate a single nucleotide polymorphism in intron 1 of the liver fatty acid-binding protein (L-FABP) gene in 156 Junmu No. 1 white swine using PCRsingle-strand conformational polymorphism. The association between the polymorphism and meat quality traits was also studied. The cloning and sequencing results indicated that the polymorphism in intron 1 was due to a T→C mutation at position 1740 of L-FABP, yielding three genotypes (TT, TC, and CC). Association analysis revealed that the polymorphism had a significant effect on marbling (P < 0.05): genotype CC had more marbling than TC, and TC had more marbling than TT. The polymorphism also had a highly significant effect on intramuscular fat content (P < 0.01). Genotypes CC and TC had higher intramuscular Y.H. Zhang et al. 236©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 12 (1): 235-241 (2013) fat content than TT; there was no significant difference between CC and TC (P > 0.05). However, no significant conclusions concerning other traits could be drawn. We tentatively conclude that L-FABP is a candidate gene or a quantitative trait locus-linked gene associated with meat quality traits.

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Investigation of porcine FABP3 and LEPR gene polymorphisms and mRNA expression for variation in intramuscular fat content

Cited by 54 publications

References 40 publications

Distribution of H-FABP and ACSL4 gene polymorphisms and their associations with intramuscular fat content and backfat thickness in different pig populations

Distribution of H-FABP and ACSL4 gene polymorphisms and their associations with intramuscular fat content and backfat thickness in different pig populations

Molecular characterization and tissue expression profile analysis of the porcine JAZF1 gene

Association of T1740C polymorphism of L-FABP with meat quality traits in Junmu No. 1 white swine

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