Distribution of H-FABP and ACSL4 gene polymorphisms and their associations with intramuscular fat content and backfat thickness in different pig populations

Chen, J N; Jiang, Yanzhi; Cen, W M; Xing, Shuhua; Zhu, Li; Tang, Guoqing; Li, Mianying; Jiang, Anan; Lou, Pinger; Wen, Anxiang; Wang, Q; He, Tao; Zhu, Guangxiang; Xie, Meng; Li, X W

doi:10.4238/2014.august.28.20

Cited by 25 publications

(19 citation statements)

References 27 publications

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“…Recent studies have shown that HFABP is related to adipogenesis in 3T3-L1 preadipocytes of the fat Banna mini-pig inbred line51. The distribution of HFABP and ACSL4 gene polymorphisms has been associated with IMF content and back fat thickness in different pig populations52. The expression of HFABP in TP-DSP was 1.78-fold higher than that in YY-LL, suggesting that it is related to the higher lipid deposition ability of TP and DSP pig breeds.…”

Section: Discussionmentioning

confidence: 99%

iTRAQ-based proteomic analysis reveals key proteins affecting muscle growth and lipid deposition in pigs

Wang

Shang

et al. 2017

Sci Rep

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Growth rate and meat quality, two economically important traits in pigs, are controlled by multiple genes and biological pathways. In the present study, we performed a proteomic analysis of longissimus dorsi muscle from six-month-old pigs from two Chinese native mini-type breeds (TP and DSP) and two introduced western breeds (YY and LL) using isobaric tag for relative and absolute quantification (iTRAQ). In total, 4,815 peptides corresponding to 969 proteins were detected. Comparison of expression patterns between TP-DSP and YY-LL revealed 288 differentially expressed proteins (DEPs), of which 169 were up-regulated and 119 were down-regulated. Functional annotation suggested that 28 DEPs were related to muscle growth and 15 to lipid deposition. Protein interaction network predictions indicated that differences in muscle growth and muscle fibre between TP-DSP and YY-LL groups were regulated by ALDOC, ENO3, PGK1, PGK2, TNNT1, TNNT3, TPM1, TPM2, TPM3, MYL3, MYH4, and TNNC2, whereas differences in lipid deposition ability were regulated by LPL, APOA1, APOC3, ACADM, FABP3, ACADVL, ACAA2, ACAT1, HADH, and PECI. Twelve DEPs were analysed using parallel reaction monitoring to confirm the reliability of the iTRAQ analysis. Our findings provide new insights into key proteins involved in muscle growth and lipid deposition in the pig.

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Section: Discussionmentioning

confidence: 99%

iTRAQ-based proteomic analysis reveals key proteins affecting muscle growth and lipid deposition in pigs

Wang

Shang

et al. 2017

Sci Rep

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“…; Chen et al . ). Significant up‐regulation of H‐FABP gene indicates the development and maturity of the adipose tissue (Li et al .…”

Section: Discussionmentioning

confidence: 97%

Effects of an immune stimulant, inactivated Mycobacterium phlei, on the growth performance as well as meat quality of fattening pigs

Zhong

Tastan

et al. 2018

Animal Science Journal

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Inactivated mycobacterium phlei (M. phlei) is well known for its immune-stimulatory functions in humans and livestock, but less information is available about the influence on meat quality of pigs when used as a feed additive. This study was designed to evaluate the effects of inactivated M. phlei on growth performance as well as meat quality of fattening pigs. A total of 240 cross-bred pigs ([Landrace × Yorkshire] × Duroc) with initial body weight of 80.14 ± 0.29 kg were randomly allocated to five treatments, each of which consisted of eight replicates with 6six pigs per replicate. The basal diet supplemented with five levels of inactivated M. phlei preparations (0, 3.5 × 10 [0.1% w/w], 7 × 10 [0.2%], 1.4 × 10 [0.4%] or 2.1 × 10 [0.6%] colony-forming units/kg) was respectively fed to the control group and four treatment groups for 30 days. Adding 0.4% of inactivated M. phlei to diet increased the average daily feed intake and average daily gain of pigs. Importantly, intramuscular fat percentage in the Longissimus dorsi (LD) was increased by feeding diet containing 0.2%, 0.4% and 0.6% of inactivated M. phlei, despite the pH value, drip loss, cooking loss and filter paper fluid uptake not being influenced. Analysis of the fatty acid components showed that some saturated fatty acids were decreased in LD after feeding inactivated M. phlei, but some monounsaturated fat acids (MUFAs) and polyunsaturated fatty acids were increased (PUFAs), which induced the total contents of MUFAs and PUFAs were improved. RT-PCR assay revealed that feeding inactivated M. phlei up-regulated genes implicated in fat metabolism in muscle, including ELOVL6, FASN, SCD1 and H-FABP. This study revealed that feeding inactivated M. phlei not only increased growth performance of fattening pigs, but also improved the meat quality by increasing intramuscular fat content, thus inactivated M. phlei probably has high utilization value in modern pig production.

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“…Gondim (2015) also reported that SNPg.240T>C when homozygous for the T allele decreases the first period of coverage, and the delivery/ parturition interval, showing that the result of the population studied in this experiment reached the apex for selection. Chen et al (2014) analyzed the H-FABP polymorphisms using PCR-RFLP using 3 restriction enzymes in six swine breeds and as the results of this study found a high predominance of one allele in at least 50% of the analyzed loci. Li et al (2006), when studying which would be the best markers for native Chinese breeds that would help in selection and conservation, showed significant differences in the frequencies of genotypes and alleles between breeds.…”

Section: Discussionmentioning

confidence: 99%

Research Article Study of genetic variability in pigs after the traditional breeding program.

Ferreira

Vieira²,

Braga

et al. 2017

Genet. Mol. Res.

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ABSTRACT. Molecular markers are tools used to improve genetic gains. The objective of this study was to analyze the security of alleles of molecular marker genes for characteristics of economic interest in a pure population of pigs. After the extraction of DNA from the hair of 272 Large White matrices, the allele and genotype frequency of single nucleotide polymorphism was performed using the ARMS-PCR Multiplex technique in the DGAT1, LEPR, H-FABP, MC4R, and SREBF1 genes using RFLP-PCR for the GH gene. After capillary electrophoresis in an automated DNA sequencing of the DGAT1, LEPR, H-FABP, and SREBF1 genes, no polymorphisms were found. Only the MC4R marker presented 100% heterozygosity.

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Distribution of H-FABP and ACSL4 gene polymorphisms and their associations with intramuscular fat content and backfat thickness in different pig populations

Cited by 25 publications

References 27 publications

iTRAQ-based proteomic analysis reveals key proteins affecting muscle growth and lipid deposition in pigs

iTRAQ-based proteomic analysis reveals key proteins affecting muscle growth and lipid deposition in pigs

Effects of an immune stimulant, inactivated Mycobacterium phlei, on the growth performance as well as meat quality of fattening pigs

Research Article Study of genetic variability in pigs after the traditional breeding program.

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