Molecular characterization and tissue expression profile analysis of the porcine JAZF1 gene

Abstract

ABSTRACT.A recent study indicated that the JAZF1 gene was related to lipid metabolism by regulating the level of gene expression in humans and mice. In order to investigate whether JAZF1 gene expression was associated with fat deposition in pig, we cloned the full-coding region of the JAZF1 gene (GenBank accession No. KF307636) from porcine longissimus dorsi. Results showed that the open reading frame of JAZF1 covered 732 bp and encoded 243 amino acids. Multiple alignment of isoform sequences revealed that the deduced amino acid sequence of JAZF1 had a high degree of sequence similarity to other vertebrates, indicating that it was highly conserved during evolution. Bioinformatic analysis indicated that pig JAZF1 contained 23 phosphorylation sites and 19 glycosyl sites. JAZF1 was predicted to have 3 ZnF-C 2 H 2 and 2 low-complexity domains. The JAZF1 mRNA expression pattern indicated that JAZF1 mRNA expression level in the liver was significantly different in 2 divergent breeds (P < 0.05). This article perhaps provided an important experimental basis for further research on the mechanisms of lipid metabolism and fat deposition in pigs.

The Diabetes Gene JAZF1 Is Essential for the Homeostatic Control of Ribosome Biogenesis and Function in Metabolic Stress

“…We first examined the expression of Jazf1 across metabolic tissues in mice fed with a chow diet. We found that Jazf1 transcript levels were most abundant in pancreatic islets, followed by adipose tissue, where expression was previously reported to be highest in all tissues (Figure S1A) (Ho et al, 2013;Yang et al, 2015).…”

Breed, Diet, and Interaction Effects on Adipose Tissue Transcriptome in Iberian and Duroc Pigs Fed Different Energy Sources

“…Recently, studies have also shown that the JAZF1 gene reduced lipid synthesis and increased lipolysis by regulating the level of expression of genes related to fat metabolism in mice [101] and because it decreases the maturation of lipid droplets and fat storage [102]. Therefore, this gene plays a critical role in the regulation of lipid homeostasis and is considered a candidate to affect meat quality [103]. The RNA-Seq results obtained for this gene, which showed a qualitative interaction, were validated by qPCR (Table 1).…”

JAZF1, a relevant metabolic regulator in type 2 diabetes

“…However, not all variants have been fully characterized. Human JAZF1 protein shares 90% homology with chimpanzee, monkey, mouse, and pig, suggesting that JAZF1 might have the similar biological function in different species …”