Investigation of plant hormone level changes in shoot tips of longan (Dimocarpus longan Lour.) treated with potassium chlorate by liquid chromatography–electrospray ionization mass spectrometry

Susawaengsup, Chanthana; Rayanakorn, Mongkon; Wongpornchai, Sugunya; Wangkarn, Sunanta

doi:10.1016/j.talanta.2011.04.073

Cited by 20 publications

(13 citation statements)

References 33 publications

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“…The expression of genes encoding ethylene biosynthetic enzymes and regulating ethylene levels in K + -deprived 8424 roots increased at 120 HAT. Susawaengsup et al (2011) found that the levels of CKs, IAA, and ABA in shoot tips of longan ( Dimocarpus longan Lour .) were increased by potassium chlorate treatment, and dry weight (DW) amounts in treated longan shoot tips were significantly higher than those of the controls.…”

Section: Discussionmentioning

confidence: 99%

Transcriptome Analysis of Differentially Expressed Genes Induced by Low and High Potassium Levels Provides Insight into Fruit Sugar Metabolism of Pear

et al. 2017

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Potassium (K) deficiency is a common abiotic stress that can inhibit the growth of fruit and thus reduce crop yields. Little research has been conducted on pear transcriptional changes under low and high K conditions. Here, we performed an experiment with 7-year-old pot-grown “Huangguan” pear trees treated with low, Control or high K levels (0, 0.4, or 0.8 g·K2O/kg soil, respectively) during fruit enlargement and mature stages. We identified 36,444 transcripts from leaves and fruit using transcriptome sequencing technology. From 105 days after full blooming (DAB) to 129 DAB, the number of differentially expressed genes (DEGs) in leaves and fruit in response to low K increased, while in response to high K, the number of DEGs in leaves and fruit decreased. We selected 17 of these DEGs for qRT-PCR analysis to confirm the RNA sequencing results. Based on GO enrichment and KEGG pathway analysis, we found that low-K treatment significantly reduced K nutrient and carbohydrate metabolism of the leaves and fruit compared with the Control treatment. During the fruit development stages, AKT1 (gene39320) played an important role on K+ transport of the leaves and fruit response to K stress. At maturity, sucrose and acid metabolic pathways were inhibited by low K. The up-regulation of the expression of three SDH and two S6PDH genes involved in sorbitol metabolism was induced by low K, promoting the fructose accumulation. Simultaneously, higher expression was found for genes encoding amylase under low K, promoting the decomposition of the starch and leading the glucose accumulation. High K could enhance leaf photosynthesis, and improve the distribution of the nutrient and carbohydrate from leaf to fruit. Sugar components of the leaves and fruit under low K were regulated by the expression of genes encoding 8 types of hormone signals and reactive oxygen species (ROS). Our data revealed the gene expression patterns of leaves and fruit in response to different K levels during the middle and late stages of fruit development as well as the molecular mechanism of improvement of fruit sugar levels by K and provided a scientific basis for improving fruit quality with supplemental K fertilizers.

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Section: Discussionmentioning

confidence: 99%

Transcriptome Analysis of Differentially Expressed Genes Induced by Low and High Potassium Levels Provides Insight into Fruit Sugar Metabolism of Pear

et al. 2017

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“…The acceleration of the flowering rate in bending shoots is accompanied by notable increases in the cytokine level in buds compared with the vertical shoots (Ito et al ., ). According to our results and previous studies, shoot bending promotes flower bud formation in ‘Fuji’ apple trees by increasing CK and ABA levels during the flower induction process, and both hormones play an active role in flower induction and flower bud formation (Han et al ., ; Susawaengsup et al ., ) (Figure ). However, our results indicated that the GA level in buds was significantly lower in the shoot‐bending treatment than in the control during flower induction (Figure c) and that GA may play a negative role in flower bud formation.…”

Section: Discussionmentioning

confidence: 99%

Shoot bending promotes flower bud formation by miRNA‐mediated regulation in apple (Malus domestica Borkh.)

Xing

Zhang

Zhao

et al. 2015

Plant Biotechnology Journal

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SummaryFlower induction in apple (Malus domestica Borkh.) trees plays an important life cycle role, but young trees produce fewer and inferior quality flower buds. Therefore, shoot bending has become an important cultural practice, significantly promoting the capacity to develop more flower buds during the growing seasons. Additionally, microRNAs (miRNAs) play essential roles in plant growth, flower induction and stress responses. In this study, we identified miRNAs potentially involved in the regulation of bud growth, and flower induction and development, as well as in the response to shoot bending. Of the 195 miRNAs identified, 137 were novel miRNAs. The miRNA expression profiles revealed that the expression levels of 68 and 27 known miRNAs were down‐regulated and up‐regulated, respectively, in response to shoot bending, and that the 31 differentially expressed novel miRNAs between them formed five major clusters. Additionally, a complex regulatory network associated with auxin, cytokinin, abscisic acid (ABA) and gibberellic acid (GA) plays important roles in cell division, bud growth and flower induction, in which related miRNAs and targets mediated regulation. Among them, miR396, 160, 393, and their targets associated with AUX, miR159, 319, 164, and their targets associated with ABA and GA, and flowering‐related miRNAs and genes, regulate bud growth and flower bud formation in response to shoot bending. Meanwhile, the flowering genes had significantly higher expression levels during shoot bending, suggesting that they are involved in this regulatory process. This study provides a framework for the future analysis of miRNAs associated with multiple hormones and their roles in the regulation of bud growth, and flower induction and formation in response to shoot bending in apple trees.

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“…Nevertheless, a single SPE process has typically been insufficient to remove lipids and other lipophilic matrix substances, preventing multiple phytohormones from being detected by HPLC-ESI-MS/MS [9,25]. Thus, two or more SPE processes have been employed to extract and purify phytohormones in complex plant matrices, but some of them suffer from low recovery and reproducibility, e.g., IAA and ABA [6,26,27]. The objectives of this study were to (1) optimize and compare, in terms of efficiency, the sample pretreatment involved in the extraction and purification of multiple phytohormones and (2) develop an efficient analytical method for their simultaneous determination in plant samples.…”

Section: Introductionmentioning

confidence: 99%

Comparison of sample pretreatment methods for the determination of multiple phytohormones in plant samples by liquid chromatography–electrospray ionization-tandem mass spectrometry

Cui

Lin

Zhou

et al. 2015

Microchemical Journal

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Investigation of plant hormone level changes in shoot tips of longan (Dimocarpus longan Lour.) treated with potassium chlorate by liquid chromatography–electrospray ionization mass spectrometry

Cited by 20 publications

References 33 publications

Transcriptome Analysis of Differentially Expressed Genes Induced by Low and High Potassium Levels Provides Insight into Fruit Sugar Metabolism of Pear

Transcriptome Analysis of Differentially Expressed Genes Induced by Low and High Potassium Levels Provides Insight into Fruit Sugar Metabolism of Pear

Shoot bending promotes flower bud formation by miRNA‐mediated regulation in apple (Malus domestica Borkh.)

Comparison of sample pretreatment methods for the determination of multiple phytohormones in plant samples by liquid chromatography–electrospray ionization-tandem mass spectrometry

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