Comparison of sample pretreatment methods for the determination of multiple phytohormones in plant samples by liquid chromatography–electrospray ionization-tandem mass spectrometry

Cui, Kunyan; Lin, Yan; Zhou, Xiaoming; Li, Shuocong; Hong, Liu; Zeng, Feng; Zhu, Fang; Ouyang, Gangfeng; Zeng, Zunxiang

doi:10.1016/j.microc.2015.02.004

Cited by 57 publications

(28 citation statements)

References 30 publications

(75 reference statements)

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“…The identification and assignation of each compounds was performed by comparing their retention times and UV spectra to authentic standards and also confirmed by an Agilent 6430 LC-MS/MS spectrometer equipped with an electrospray ionization source. The electrospray ionization mass spectrometry detection was performed in negative mode with the following optimized parameters: capillary temperature 400°C; drying gas N 2 12 L/min; nebulizer pressure, 45 psi (Fecka and Turek 2008;Cui et al 2015). Data gaining was performed using multiple reactions monitoring (MRM) method that only monitors specific mass transitions during preset retention times.…”

Section: Lc-dad-esi-ms/ms Analysismentioning

confidence: 99%

Characterization of aroma-active and phenolic profiles of wild thyme (Thymus serpyllum) by GC-MS-Olfactometry and LC-ESI-MS/MS

2015

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The present study was designed to characterize the volatile, aroma-active and phenolic compounds of wild thyme. Volatile components of T. serpyllum were extracted by use of the purge and trap technique with dichloromethane and analyzed by gas chromatographymass spectrometry (GC-MS). The extraction method gave highly representative aromatic extract of the studied sample based on the sensory analysis. A total of 24 compounds were identified and quantified in Thymus serpyllum. Terpenes were qualitatively and quantitatively the most dominant volatiles in the sample. Aroma extract dilution analysis (AEDA) was used for the first time for the determination of aroma-active compounds of Thymus serpyllum. In total, 12 aroma-active compounds were detected in the aromatic extract by GC-MS-Olfactometry and terpenes were the most abundant compounds. Highperformance liquid chromatography/electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS) method was used for the phenolic compounds analysis. 18 phenolic compounds were identified and quantified in the T. serpyllum. Luteolin 7-O-glucoside, luteolin and rosmarinic acid were the most abundant phenolics in this herb.

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Section: Lc-dad-esi-ms/ms Analysismentioning

confidence: 99%

Characterization of aroma-active and phenolic profiles of wild thyme (Thymus serpyllum) by GC-MS-Olfactometry and LC-ESI-MS/MS

2015

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“…The ion source allows large, non-volatile molecules to be analyzed directly from the liquid phase, which ionizes the neutral atom or molecule and fragment the ion beam generated from it [93]. Two of the frequently used ion sources in plant hormone analysis are electrospray ionization (ESI) and atmospheric pressure chemical ionization (APCI).…”

Section: Lc-ms Analysismentioning

confidence: 99%

Recent developments and emerging trends of mass spectrometric methods in plant hormone analysis: a review

et al. 2020

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Plant hormones are naturally occurring small molecule compounds which are present at trace amounts in plant. They play a pivotal role in the regulation of plant growth. The biological activity of plant hormones depends on their concentrations in the plant, thus, accurate determination of plant hormone is paramount. However, the complex plant matrix, wide polarity range and low concentration of plant hormones are the main hindrances to effective analyses of plant hormone even when state-of-the-art analytical techniques are employed. These factors substantially influence the accuracy of analytical results. So far, significant progress has been realized in the analysis of plant hormones, particularly in sample pretreatment techniques and mass spectrometric methods. This review describes the classic extraction and modern microextraction techniques used to analyze plant hormone. Advancements in solid phase microextraction (SPME) methods have been driven by the ever-increasing requirement for dynamic and in vivo identification of the spatial distribution of plant hormones in real-life plant samples, which would contribute greatly to the burgeoning field of plant hormone investigation. In this review, we describe advances in various aspects of mass spectrometry methods. Many fragmentation patterns are analyzed to provide the theoretical basis for the establishment of a mass spectral database for the analysis of plant hormones. We hope to provide a technical guide for further discovery of new plant hormones. More than 140 research studies on plant hormone published in the past decade are reviewed, with a particular emphasis on the recent advances in mass spectrometry and sample pretreatment techniques in the analysis of plant hormone. The potential progress for further research in plant hormones analysis is also highlighted.

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“…Liquid–liquid extraction (LLE) and liquid–liquid microextraction (LLME), hollow fibre-based liquid–liquid–liquid microextraction (HF–LLLME) and dispersive liquid–liquid microextraction (DLLME) have been used previously for IAA purification [14–20]. Solid-phase extraction (SPE) and antibody-based immune methods have recently become widely used to extract and purify IAA [6, 21–26]. Optimized solid-phase microextraction (SPME) and double-layered SPE (DL/SPE) resulted in a higher level of recovery and better ability to remove pigments [27, 28].…”

Section: Introductionmentioning

confidence: 99%

“…Since modern SPE/SPME techniques are usually applied online coupled with HPLC, the current emphasis involves choosing various sorbents for trapping analytes [30, 31]. Many commercial SPE/SPME columns based on different sorbents have been widely used for high-throughput phytohormone extraction and purification from crude plant extracts [22, 23, 26]. However, commercial SPE columns for multiple phytohormones, such as Sep-Pak C18, Oasis HLB, Oasis MCX and Oasis MAX, are not designed specifically for phytohormones such as IAA.…”

Section: Introductionmentioning

confidence: 99%

Auxin Extraction and Purification Based on Recombinant Aux/IAA Proteins

Luo

Chen

et al. 2017

Biol Proced Online

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BackgroundIndole-3-acetic acid (IAA) extraction and purification are of great importance in auxin research, which is a hot topic in the plant growth and development field. Solid-phase extraction (SPE) is frequently used for IAA extraction and purification. However, no IAA-specific SPE columns are commercially available at the moment. Therefore, the development of IAA-specific recognition materials and IAA extraction and purification methods will help researchers meet the need for more precise analytical methods for research on phytohormones.ResultsSince the AUXIN RESISTANT/INDOLE-3-ACETIC ACID INDUCIBLE (Aux/IAA) proteins show higher specific binding capability with auxin, recombinant IAA1, IAA7 and IAA28 proteins were used as sorbents to develop an IAA extraction and purification method. A GST tag was used to solidify the recombinant protein in a column. Aux/IAA proteins solidified in a column have successfully trapped trace IAA in aqueous solutions. The IAA7 protein showed higher IAA binding capability than the other proteins tested. In addition, expression of the IAA7 protein in Drosophila Schneider 2 (S2) cells produced better levels of binding than IAA7 expressed in E. coli.ConclusionThis work validated the potential of Aux/IAA proteins to extract and purify IAA from crude plant extracts once we refined the techniques for these processes.Electronic supplementary materialThe online version of this article (doi:10.1186/s12575-016-0050-1) contains supplementary material, which is available to authorized users.

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Comparison of sample pretreatment methods for the determination of multiple phytohormones in plant samples by liquid chromatography–electrospray ionization-tandem mass spectrometry

Cited by 57 publications

References 30 publications

Characterization of aroma-active and phenolic profiles of wild thyme (Thymus serpyllum) by GC-MS-Olfactometry and LC-ESI-MS/MS

Characterization of aroma-active and phenolic profiles of wild thyme (Thymus serpyllum) by GC-MS-Olfactometry and LC-ESI-MS/MS

Recent developments and emerging trends of mass spectrometric methods in plant hormone analysis: a review

Auxin Extraction and Purification Based on Recombinant Aux/IAA Proteins

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