Investigation of Obesity-Alleviation Effect of <i>Eurycoma longifolia</i> on Mice Fed with a High-Fat Diet through Metabolomics Revealed Enhanced Decomposition and Inhibition of Accumulation of Lipids

Zhang, Dingkun; Zheng, Wen; Li, Xin; Ge, Liang; Yang, Nan; Liu, Yueqiu; Li, Ang; Liu, Xin; Zhang, Rui; Cheng, Jun; Yang, Hao; Gong, Meng

doi:10.1021/acs.jproteome.1c00015

Cited by 7 publications

(12 citation statements)

References 44 publications

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“…The metabolite analysis was based on literature reports [ 13 , 14 ] in sample preparation and metabolite analysis. Briefly, an Ultra-high performance liquid phase tandem Thermo Q Exactive Orbitrap mass spectrometer, which was equipped with a UPLC HSS T3 column (2.1 mm × 100 mm, 1.8 μm) (Waters Corporation, Shanghai, China), and the control software (Xcalibur, version: 4.0.27, Thermo Scientific (Waltham, MA, USA)) was used as the liquid-mass system for primary and secondary mass spectrometry data acquisition.…”

Section: Methodsmentioning

confidence: 99%

Torularhodin Alleviates Hepatic Dyslipidemia and Inflammations in High-Fat Diet-Induced Obese Mice via PPARα Signaling Pathway

Cheng²,

Li³

et al. 2022

Molecules

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Torularhodin is a β-carotene-like compound from Sporidiobolus pararoseus, and its protective effect against high-fat diet (HFD)-induced hepatic dyslipidemia and inflammation was investigated. Compared to mice of C57BL/6J fed on HFD, the addition of Torularhodin into the HFD (HFD-T) significantly reduced body weight, serum triglyceride (TG), total cholesterol (TC), low-density lipoprotein (LDL), and the inflammatory mediators of TNF-α, IL-6, IL-1β, and lipopolysaccharide (LPS). A significant increase of high-density lipoprotein cholesterol (HDL-c), which is beneficial to cholesterol clearance, was also observed in HFD-T group. Proteomic analysis showed HDL-C-c is highly correlated with proteins (e.g., CPT1A and CYP7A1) involved in lipid β-oxidation and bile acid synthesis, whereas the other phenotypic parameters (TC, TG, LDL, and inflammatory cytokines) are highly associated with proteins (e.g., SLC27A4) involved in lipid-uptake. The up-regulated anti-inflammation proteins FAS, BAX, ICAM1, OCLN, GSTP1, FAF1, LRP1, APEX1, ROCK1, MANF, STAT3, and INSR and down-regulated pro-inflammatory proteins OPTN, PTK2B, FADD, MIF, CASP3, YAP1, DNM1L, and NAMPT not only demonstrate the occurrence of HFD-induced hepatic inflammation, but also prove the anti-inflammatory property of Torularhodin. KEGG signaling pathway analysis revealed that the PPARα signaling pathway is likely fundamental to the health function of Torularhodin through up-regulating genes related to fatty acid β-oxidation, cholesterol excretion, HDL-Cc formation, and anti-inflammation. Torularhodin, as a new food resource, may act as a therapeutic agent to prevent hepatic dyslipidemia and related inflammation for improved health.

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Section: Methodsmentioning

confidence: 99%

Torularhodin Alleviates Hepatic Dyslipidemia and Inflammations in High-Fat Diet-Induced Obese Mice via PPARα Signaling Pathway

Cheng²,

Li³

et al. 2022

Molecules

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“…In other studies, DESI-MS has been applied to image cranial mesenchyme of mouse embryos, 119 to the analysis of liver and lung tissues in combination with the elemental mapping technique particle-induced X-ray emission, 120 to study lipid profiles in human hippocampus tissue from patients with temporal lobe epilepsy, 121 and mouse tissues following different diet treatments. 122 Over time, modifications have been made to DESI to improve weaknesses of the technique, one of these alternative techniques is AFADESI. In the diagnostic study of biological tissues, lipids can play an important role in the study of diseases such as cancer.…”

Section: Biological Materialsmentioning

confidence: 99%

“…The study also compared results with those from native LESA‐MS experiments, demonstrating no additional error resulting from the use of TWIMS. In other studies, DESI‐MS has been applied to image cranial mesenchyme of mouse embryos, 119 to the analysis of liver and lung tissues in combination with the elemental mapping technique particle‐induced X‐ray emission, 120 to study lipid profiles in human hippocampus tissue from patients with temporal lobe epilepsy, 121 and mouse tissues following different diet treatments 122 …”

Section: Applications Of Ambient Ionization Mass Spectrometrymentioning

confidence: 99%

Applications of ambient ionization mass spectrometry in 2021: An annual review

Rankin‐Turner

Reynolds

Turner

et al. 2022

Analytical Science Advances

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Ambient ionization mass spectrometry (AIMS) has revolutionized the field of analytical chemistry, enabling the rapid, direct analysis of samples in their native state. Since the inception of AIMS almost 20 years ago, the analytical community has driven the further development of this suite of techniques, motivated by the plentiful advantages offered in addition to traditional mass spectrometry. Workflows can be simplified through the elimination of sample preparation, analysis times can be significantly reduced and analysis remote from the traditional laboratory space has become a real possibility. As such, the interest in AIMS has rapidly spread through analytical communities worldwide, and AIMS techniques are increasingly being integrated with standard laboratory operations. This annual review covers applications of AIMS techniques throughout 2021, with a specific focus on AIMS applications in a number of key fields of research including disease diagnostics, forensics and security, food safety testing and environmental sciences. While some new techniques are introduced, the focus in AIMS research is increasingly shifting from the development of novel techniques toward efforts to improve existing AIMS techniques, particularly in terms of reproducibility, quantification and ease-of-use.

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“…In recent years, metabolomics-based approaches have been used to gain insight into the therapeutic effects of functional foods 7 or herbal medicines and their mechanism of action against metabolic diseases. 8,9 In this study, by using the ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) platform, a metabolomics-based approach was used to investigate the hypoglycemic activity and its effect on alleviating diabetic complications of Ficus racemosa fruit in diabetic mice, and to elucidate the underlying mechanism of action, which would supply scientific support for its folk use.…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Metabolomics-based evidence of the hypoglycemic effect and alleviation of diabetic complications by Ficus racemosa fruit in diabetic mice

Liu¹,

Wen

Zhang

et al. 2022

Food Funct.

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Investigation of Obesity-Alleviation Effect of Eurycoma longifolia on Mice Fed with a High-Fat Diet through Metabolomics Revealed Enhanced Decomposition and Inhibition of Accumulation of Lipids

Cited by 7 publications

References 44 publications

Torularhodin Alleviates Hepatic Dyslipidemia and Inflammations in High-Fat Diet-Induced Obese Mice via PPARα Signaling Pathway

Torularhodin Alleviates Hepatic Dyslipidemia and Inflammations in High-Fat Diet-Induced Obese Mice via PPARα Signaling Pathway

Applications of ambient ionization mass spectrometry in 2021: An annual review

Metabolomics-based evidence of the hypoglycemic effect and alleviation of diabetic complications by Ficus racemosa fruit in diabetic mice

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