Investigation of Growth Hormone Releasing Hormone, Growth Hormone and Prolactin Hormone Gene Polymorphism in Anatolian Water Buffalo

Konca, Mehmet Akif; Akyüz, Bilal

doi:10.1515/aoas-2016-0100

Cited by 8 publications

(12 citation statements)

References 5 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…If it does not meet the criteria, the gene is monomorphic (Figures 4 and 5). Genetic diversity in a population can be used as a parameter in studying population genetics and evolution (Szatkowska et al 2009;Konca and Akyuz 2017). In addition, it can also be used to identify and preserve individuals in the population associated with special characters.…”

Section: Gh Dan Ghrh Genes Polymorphismmentioning

confidence: 99%

Body size characteristics and polymorphism in GH and GHRH genes of Simeulue Buffalo of Aceh, Indonesia

Eriani

Rahmi

Jamil³

et al. 2018

Biodiversitas

View full text Add to dashboard Cite

Eriani K, Rahmi R, Jamil I, Rosnizar R, Azhar A. 2019. Body size characteristics and polymorphism in GH and GHRH genes of Simeulue Buffalo of Aceh, Indonesia. Biodiversitas 20: 236-242. This study was done to determine body size characteristics, to identify the presence of polymorphism in the GH/MspI and GHRH/HaeIII genes, and to analyze the relationship between GH and GHRH gene polymorphisms to body size characteristics in Simeulue buffalo. Experimental animals used were 45 Simeulue buffalo randomly sampled from three subdistricts, i.e., Salang, Alafan and Simeulue Cut, fifteen from each subdistrict. Body size characteristics measured were withered height, body length, chest circumference, chest depth, and hip height. A 5 mL whole blood was collected from fifteen out of forty five by using EDTA as an anticoagulant. Extraction of genomic DNA and analysis of GH/MspI and GHRH/HaeIII polymorphisms was done using GeneAid DNA extraction kit and polymerase chain reaction-restriction length fragment polymorphism (PCR-RFLP) method, respectively. The data of body size characteristics which includes mean value, standard deviation, and coefficient of variation were analyzed descriptively. The molecular data represented by DNA banding pattern was used to calculate allele and genotype frequencies, expected (He) and observed (Ho) heterozygosities, and Hardy-Weinberg equilibrium. The relationship between genotype and body size characteristic was analyzed using t-test. The results showed that coefficient of variation in body size characteristics was low, ranging from 5.39-16.06% in the age category of 4-6 years old and ranging from 5.25-11.27% in the age category of 2- 4 years old. The results of the molecular analysis indicated that GH/MspI locus was monomorphic by the presence of one (+/+) genotype. GHRH/HaeIII locus was polymorphic as shown by the presence of 3 genotypes namely AA (0.13), AB (0.80) and BB (0.07). Expected heterozygosity (0.80) was higher than observed heterozygosity (0.49). The frequency of GHRH/HaeIII agreed with Hardy Weinberg equilibrium. GHRH/HaeIII polymorphism did not significantly influence (P>0.05) body size characteristics of Simeulue buffalo.

show abstract

Section: Gh Dan Ghrh Genes Polymorphismmentioning

confidence: 99%

Body size characteristics and polymorphism in GH and GHRH genes of Simeulue Buffalo of Aceh, Indonesia

Eriani

Rahmi

Jamil³

et al. 2018

Biodiversitas

View full text Add to dashboard Cite

show abstract

“…In another study conducted on 126 Anatolian water buffaloes in Turkey, GH gene polymorphisms were determined as LL (75.5%), VV (1.7%), and LV (22.8%) genotypes, and the L allele frequency was reported as 87% (Konca and Akyüz, 2017). In this study, more animals were used, but the VV genotype was not determined.…”

Section: Discussionmentioning

confidence: 84%

Investigation of GH and GHRAlu I gene polymorphisms on meat yields in Anatolian water buffalo breed using PCR-RFLP method

Özşensoy¹,

Kara²

2019

Turk J Zool

View full text Add to dashboard Cite

The Anatolian water buffalo is a native water buffalo breed only being reared in Turkey. The objective of this study was to investigate gene polymorphisms on exons 4 and 5 of the growth hormone (GH) and on exon 10 of the growth hormone receptor (GHR), which are thought to be related to meat yields of Anatolian water buffalo. Blood samples of 192 water buffaloes from Sivas Province were used in DNA extractions by phenol-chloroform method. DNA samples were amplified by using specific primers in PCR. PCR products were digested by Alu I (GH, n: 167) and Alu I (GHR, n: 192) restriction enzymes in order to determine polymorphisms. Digested PCR products were separated in 2.5%-3% agarose gel electrophoresis to determine allelic polymorphisms. As a result, the LL (78.44%) and LV (21.56%) genotypes and L (0.89) and V (0.11) alleles for the GH gene and the AA (7.81%) and AG (92.19%) genotypes and A (0.54) and G (0.46) alleles for the GHR gene were obtained. Gene polymorphisms were not detected (P > 0.05) on the GH gene, but a significant difference was found for the GHR gene (P < 0.001). Therefore, it can be said that Anatolian water buffaloes have increased meat yield due to the presence of genotypes in gene regions. This was the first investigation of enzyme polymorphisms of the GHR gene in Anatolian water buffaloes.

show abstract

“…Polymorphism investigations in both cattle and water buffalo breeds were carried out for PRL exons. There are studies for exon 3 (Mitra et al, 1995;Kaplan and Boztepe, 2000;Alipanah et al, 2007;Kumari et al, 2008;Biradar et al, 2014;Konca and Akyüz, 2017) and for exon 1 (Ladani et al, 2003b;Madnalwar et al, 2010). However, PRL exon 3 has been the most extensively studied.…”

Section: Discussionmentioning

confidence: 99%

“…Up to this point, only the HaeIII RE is used for digesting the PRL exon 1. There are only two previous studies conducted with Anatolian water buffalo breed for PRL gene polymorphisms and only exon 3 was investigated (Kaplan and Boztepe, 2000;Konca and Akyüz, 2017).…”

Section: Discussionmentioning

confidence: 99%

“…The Anatolian water buffalo was declared as one of the national genetic resource in 2004 (Governmental Newspaper, 2004). There have been very sparse studies regarding molecular characterization of Anatolian water buffalo breed (Kaplan and Boztepe, 2000;Konca and Akyüz, 2017). Therefore, there is a need for the molecular characterization of Anatolian water buffalo breed.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Investigation of PRL-RsaI and HaeIII gene polymorphisms in Anatolian water buffaloes bred by using PCR-RFLP method

Özşensoy

2018

R. Bras. Zootec.

View full text Add to dashboard Cite

The objective of this study was to investigate polymorphisms both in exons 1 and 3 of prolactin (PRL) gene for milk productivity of Anatolian water buffalo breed in Sivas province in Turkey. Blood samples were collected from 129 male and female water buffaloes and DNA was isolated by using phenol/chloroform method. Samples of DNA were amplified and resulting PCR products were digested with RsaI (for exons 1 and 3) and HaeIII (for exon 1). Allelic polymorphisms were determined by separation of fragments obtained from digested PCR products in 3% agarose gel electrophoresis. AA genotype (HaeIII) and BB genotype (RsaI) of exon 1 and only AA genotype (RsaI) of exon 3 were obtained. No polymorphisms were determined in Anatolian water buffalo breed and all loci were found as monomorphic. It can be stated that Anatolian water buffalo has a higher milk and milk fat yields since BB genotype was obtained.

show abstract

Investigation of Growth Hormone Releasing Hormone, Growth Hormone and Prolactin Hormone Gene Polymorphism in Anatolian Water Buffalo

Cited by 8 publications

References 5 publications

Body size characteristics and polymorphism in GH and GHRH genes of Simeulue Buffalo of Aceh, Indonesia

Body size characteristics and polymorphism in GH and GHRH genes of Simeulue Buffalo of Aceh, Indonesia

Investigation of GH and GHRAlu I gene polymorphisms on meat yields in Anatolian water buffalo breed using PCR-RFLP method

Investigation of PRL-RsaI and HaeIII gene polymorphisms in Anatolian water buffaloes bred by using PCR-RFLP method

Contact Info

Product

Resources

About